Detection of inflammatory markers in stools from patients with irritable bowel syndrome and collagenous colitis

Lettesjö, Helene; Hansson, Tomas; Peterson, Christer; Ung, Kjell-Arne; Ringström, Gisela; Abrahamsson, Hasse; Simrén, Magnus

doi:10.1080/00365520510023909

Cited by 53 publications

(49 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low-grade inflammation involving mast cells has previously been observed in some patients with IBS [14, 32]. However, in the present study, we failed to detect an increased local mast cell activity in the IBS patients as well as in most of the other patients.…”

Section: Discussioncontrasting

confidence: 55%

“…Eosinophil protein X (EPX), one of the major proteins released by eosinophils [12], and tryptase, one of the major proteins released by mast cells during allergic reactions, can be accurately measured in feces [13, 14]. In a previous study including adult patients with food hypersensitivity, we observed an overall increase in the fecal levels of EPX (F-EPX) over time during periods of variable symptoms [15].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Measurements of Eosinophil Activation before and after Food Challenges in Adults with Food Hypersensitivity

Odijk

Peterson²,

Ahlstedt³

et al. 2006

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Objective assessment of inflammatory reactions in the gastrointestinal tract could be useful in the diagnosis of food hypersensitivity. The aim of the present study was to investigate the involvement of eosinophils and mast cells in the inflammatory response of patients with food hypersensitivity before and after food challenges. Methods: Eleven patients (4 with IgE-mediated allergy and 7 without) with food hypersensitivity and positive double-blind, placebo-controlled food challenge were subjected to food challenge in a single-blinded fashion. Four subjects with no known food hypersensitivity were recruited as controls. Placebo was given after a 1-week washout period followed by an active dose. Stool, urinary and serum samples were collected and symptoms were recorded in a diary. Fecal samples were analyzed for eosinophil protein X (F-EPX) and tryptase; urinary samples for EPX (U-EPX) and leukotriene E₄ (U-LTE₄) and serum samples were analyzed for eotaxin and food-specific IgE antibodies. Results: Patients with IgE-mediated food allergy had increased levels of F-EPX compared to controls and tended to have lower serum levels of eotaxin compared to non-allergic patients and controls. U-LTE₄ was significantly higher in allergic patients compared to non-allergic patients after challenge. Moreover, F-EPX correlated to U-LTE₄ (p = 0.011). Reported symptoms, abdominal pain, distension, flatulence and nausea were similar in the allergic and non-allergic patients. Conclusion: The results strongly indicate that eosinophils are activated in the gastrointestinal tract of food-allergic patients but not in patients with non-allergic food hypersensitivity. Due to the inconsistent pattern of symptoms after placebo and active food challenge, it was not possible to relate the levels of inflammation markers to the recorded symptoms.

show abstract

Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Measurements of Eosinophil Activation before and after Food Challenges in Adults with Food Hypersensitivity

Odijk

Peterson²,

Ahlstedt³

et al. 2006

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Cytokine assays have revealed no major changes in the basal levels of cytokines in IBS patients [31][32][33][34] with the exception of IL6 and IL8, which have been found to be moderately increased in some studies. 31,34,35 A decrease in the levels of the antiinflammatory cytokine IL10 has also recently been reported in children with IBS, 36 although this decrease was not observed in adults.…”

Section: Resultsmentioning

confidence: 99%

Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Matricon

Méleine

Gelot

et al. 2012

Aliment Pharmacol Ther

187

180

View full text Add to dashboard Cite

This uncommissioned review article was subject to full peer-review. SUMMARY BackgroundIrritable bowel syndrome (IBS), one of the most common gastrointestinal disorders, markedly impairing patients' quality of life. Drug development for IBS treatment has been hampered by the lack of understanding of IBS aetiology. In recent years, numerous data have emerged that suggest the involvement of immune activation in IBS, at least in a subset of patients. AimTo determine whether immune activation and intestinal permeabilisation are more frequently observed in IBS patients compared with healthy controls. MethodsThe scientific bibliography was searched using the following keywords: irritable bowel syndrome, inflammation, immune activation, permeabilisation, intestine, assay, histology and human. The retrieved studies, including blood, faecal and histological studies, were analysed to provide a comprehensive and structured overview of the available data including the type of assay, type of inflammatory marker investigated or intestinal segment studied. ResultsImmune activation was more frequently observed in IBS patients than in healthy controls. An increase in the number of mast cells and lymphocytes, an alteration in cytokine levels and intestinal permeabilisation were reported in IBS patients. No consistent changes in the numbers of B cells or enterochromaffin cells or in mucosal serotonin production were demonstrated. ConclusionsThe changes observed were modest and often heterogeneous among the studied population. Only appropriate interventions improving irritable bowel syndrome symptoms could highlight and confirm the role of immune activation in this pathophysiology.

show abstract

“…For example, eosinophil protein X (EPX), myeloperoxidase (MPO), tryptase, interleukin 1β or tumor necrosis factor α measured in supernatants from processed feces of patients with IBS were not elevated, in contrast to positive (IBD) controls. 6 Similarly, increased CD3 lymphocytes are observed in colonic biopsies. 7 or mast cells are increased in colonic or ileal biopsies in some studies; 8-10 however, other studies did not confirm these findings e.g., mast cell numbers are not increased in the colonic biopsies from patients with post-dysentery IBS.…”

Section: Introductionmentioning

confidence: 94%

Alterations in Mucosal Immunity Identified in the Colon of Patients With Irritable Bowel Syndrome

Aerssens

Camilleri

Talloen

et al. 2008

Clinical Gastroenterology and Hepatology

113

View full text Add to dashboard Cite

show abstract

Detection of inflammatory markers in stools from patients with irritable bowel syndrome and collagenous colitis

Cited by 53 publications

References 25 publications

Measurements of Eosinophil Activation before and after Food Challenges in Adults with Food Hypersensitivity

Measurements of Eosinophil Activation before and after Food Challenges in Adults with Food Hypersensitivity

Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Alterations in Mucosal Immunity Identified in the Colon of Patients With Irritable Bowel Syndrome

Contact Info

Product

Resources

About