Detection of influenza A viruses with a portable real-time PCR instrument

Mölsä, Markos; Koskela, Katja A.; Rönkkö, Esa; Ikonen, Niina; Ziegler, Thedi; Nikkari, Simo

doi:10.1016/j.jviromet.2012.02.001

Cited by 20 publications

(9 citation statements)

References 10 publications

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“…120 BioFire has adapted its cartridge technology to develop a portable PCR unit for biodefense purposes. 121,122 Genedrive (Epistem, Manchester, United Kingdom) is a portable machine whose cartridges use a paper-based extraction system that has so far been evaluated for tuberculosis diagnosis with inconsistent results. 85,86 Biomeme (Philadelphia, PA) is developing a handheld PCR device that runs off of an iPhone, using its camera for fluorometric detection and screen for interface.…”

Section: Traditional Microbiology Techniques Reinvent Themselvesmentioning

confidence: 99%

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Robinson

Manabe

2017

The American Society of Tropical Medicine and Hygiene

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Diagnosing the cause of acute febrile illness in resource-limited settings is important-to give the correct antimicrobials to patients who need them, to prevent unnecessary antimicrobial use, to detect emerging infectious diseases early, and to guide vaccine deployment. A variety of approaches are yielding more rapid and accurate tests that can detect more pathogens in a wider variety of settings. After decades of slow progress in diagnostics for acute febrile illness in resource-limited settings, a wave of converging advancements will enable clinicians in resource-limited settings to reduce uncertainty for the diagnosis of acute febrile illness.

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Section: Traditional Microbiology Techniques Reinvent Themselvesmentioning

confidence: 99%

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Robinson

Manabe

2017

The American Society of Tropical Medicine and Hygiene

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“…The RAZOR EX Instrument is a commercially available, non-microfluidic portable RT-PCR bio-detection system (BioFire Defense, Salt Lake City, Utah ) . It detected influenza virus A in clinical samples within 90 min run time, including sample preparation (Mölsä et al 2012 ). According to the Biofire Defense website ( http://biofiredefense.com ), the platform can be used to detect Campylobacter , L. monocytogenes and Salmonella .…”

Section: Future Trendsmentioning

confidence: 99%

Trends and advances in food analysis by real-time polymerase chain reaction

et al. 2016

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Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns. Real-time polymerase chain reaction (RT-PCR), a rapid and inexpensive quantitative method to detect the presence of targeted DNA-segments in samples, helps in determining both accidental and intentional adulterations of foods by biological contaminants. This review presents recent developments in theory, techniques, and applications of RT-PCR in food analyses, RT-PCR addresses the limitations of traditional food analyses in terms of sensitivity, range of analytes, multiplexing ability, cost, time, and pointof-care applications. A range of targets, including species of plants or animals which are used as food ingredients, food-borne bacteria or viruses, genetically modified organisms, and allergens, even in highly processed foods can be identified by RT-PCR, even at very low concentrations. Microfluidic RT-PCR eliminates the separate sample-processing step to create opportunities for point-of-care analyses. We also cover the challenges related to using RT-PCR for food analyses, such as the need to further improve sample handling.

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“…Arif et al [25] has demonstrated that biotinylated primer has no adverse effect on PCR amplification and sensitivity. The detection performances of the Razor Ex system and standard real-time qPCR technology (ABI 7300) were compared for specific detection of the causal agents of anthrax, brucellosis, tularemia, and plague [23] as well as influenza A viruses [24]. In our hands, the Razor Ex detected P. omnivora [25], High plains virus [31], Xfp and Xf with high assay specificity.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Reliability and Accuracy for Field Deployable Bioforensic Detection and Discrimination of Xylella fastidiosa subsp. pauca, Causal Agent of Citrus Variegated Chlorosis Using Razor Ex Technology and TaqMan Quantitative PCR

et al. 2013

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A reliable, accurate and rapid multigene-based assay combining real time quantitative PCR (qPCR) and a Razor Ex BioDetection System (Razor Ex) was validated for detection of Xylella fastidiosa subsp. pauca (Xfp, a xylem-limited bacterium that causes citrus variegated chlorosis [CVC]). CVC, which is exotic to the United States, has spread through South and Central America and could significantly impact U.S. citrus if it arrives. A method for early, accurate and sensitive detection of Xfp in plant tissues is needed by plant health officials for inspection of products from quarantined locations, and by extension specialists for detection, identification and management of disease outbreaks and reservoir hosts. Two sets of specific PCR primers and probes, targeting Xfp genes for fimbrillin and the periplasmic iron-binding protein were designed. A third pair of primers targeting the conserved cobalamin synthesis protein gene was designed to detect all possible X. fastidiosa (Xf) strains. All three primer sets detected as little as 1 fg of plasmid DNA carrying X. fastidiosa target sequences and genomic DNA of Xfp at as little as 1 - 10 fg. The use of Razor Ex facilitates a rapid (about 30 min) in-field assay capability for detection of all Xf strains, and for specific detection of Xfp. Combined use of three primer sets targeting different genes increased the assay accuracy and broadened the range of detection. To our knowledge, this is the first report of a field-deployable rapid and reliable bioforensic detection and discrimination method for a bacterial phytopathogen based on multigene targets.

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Detection of influenza A viruses with a portable real-time PCR instrument

Cited by 20 publications

References 10 publications

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Trends and advances in food analysis by real-time polymerase chain reaction

Enhanced Reliability and Accuracy for Field Deployable Bioforensic Detection and Discrimination of Xylella fastidiosa subsp. pauca, Causal Agent of Citrus Variegated Chlorosis Using Razor Ex Technology and TaqMan Quantitative PCR

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