Detection of Intestinal Ischemia Using a Microdialysis Technique in an Animal Model

Sommer, Thorbjørn; Larsen, Julie Brogaard

doi:10.1007/s00268-002-6594-z

Cited by 41 publications

(36 citation statements)

References 24 publications

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“…Sommer et al compared results from microdialysis probes inserted intraperitoneally, intramurally and intraluminally in the bowel of a swine model (126) (Figure 6). Insertion of the probe intramurally provides faster detection of metabolic changes than intraluminal and peritoneal microdialysis due to the proximity of the probe to the damaged tissue and the lack of dilution artifacts (130). However, this is more invasive and presents a difficult challenge for the clinician thus requiring intensive training.…”

Section: Intestinal Microdialysismentioning

confidence: 99%

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Priya¹,

Córcoles²

2012

Sepsis - An Ongoing and Significant Challenge

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Section: Intestinal Microdialysismentioning

confidence: 99%

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Priya¹,

Córcoles²

2012

Sepsis - An Ongoing and Significant Challenge

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“…In this study the tissue of interest was the peritoneal cavity. The substances studied-glucose, glycerol, lactate, and pyruvate [1][2][3] -are related to cell metabolism and integrity.…”

mentioning

confidence: 99%

Intraperitoneal Microdialysis as a Monitoring Method in the Intensive Care Unit

Konstantinos

Komnos

Paraforos

et al. 2014

International Surgery

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Studies on surgical patients provide some evidence of prompt detection of enteric ischemia with microdialysis. The purpose of the study was to measure intraperitoneal microdialysis values (glucose, glycerol, pyruvate, and lactate) in patients hospitalized in an intensive care unit (ICU) with an underlying abdominal surgical condition and to correlate these values with patients' outcomes. Twenty-one patients, 10 female, were enrolled in the study. The intraperitoneal metabolite values were measured for 3 consecutive days, starting from the first day of ICU hospitalization. Descriptive and inferential statistics were performed. The t-test, repeated measures analysis, Holm's test, and a logistic regression model were applied. Level of statistical significance was set at P ¼ 0.05. Mean age of participants was 68.10 6 8.02 years old. Survivors exhibited statistically significantly higher glucose values on day 3 (6.61 6 2.01 against 3.67 6 1.62; P ¼ 0.002). Mean lactate/ pyruvate (L/P) values were above 20 (35.35 6 27.11). All nonsurvivors had a mean three day L/P values greater than 25.94. Low L/P values were related to increased survival possibilities. High microdialysis glucose concentration, high L/P ratio and low glucose concentration were the major findings during the first three ICU hospitalization days in non-survivors. Intraperitoneal microdialysis may serve as a useful tool in understanding enteric ischemia pathophysiology.

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“…A real-time alternative is microdialysis, a procedure in which the surgeon penetrates the intact tissue with a needle ~2 mm in diameter in order to sample the extracellular environment for ischemia by-products. Both of these options are invasive and can alter local tissue hemodynamics and metabolism [1]. When dealing with tissue of critical or unknown status, a noninvasive method is preferred.…”

Section: Introductionmentioning

confidence: 99%

Optical spectroscopy approach for the predictive assessment of kidney functional recovery following ischemic injury

et al. 2010

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Tissue that has undergone significant yet unknown amount of ischemic injury is frequently encountered in organ transplantation and trauma clinics. With no reliable real-time method of assessing the degree of injury incurred in tissue, surgeons generally rely on visual observation which is subjective. In this work, we investigate the use of optical spectroscopy methods as a potentially more reliable approach. Previous work by various groups was strongly suggestive that tissue autofluorescence from NADH obtained under UV excitation is sensitive to metabolic response changes. To test and expand upon this concept, we monitored autofluorescence and light scattering intensities of injured vs. uninjured rat kidneys via multimodal imaging under 355 nm, 325 nm, and 266 nm excitation as well as scattering under 500 nm illumination. 355 nm excitation was used to probe mainly NADH, a metabolite, while 266 nm excitation was used to probe mainly tryptophan to correct for non-metabolic signal artifacts. The ratio of autofluorescence intensities derived under these two excitation wavelengths was calculated and its temporal profile was fit to a relaxation model. Time constants were extracted, and longer time constants were associated with kidney dysfunction. Analysis of both the autofluorescence and light scattering images suggests that changes in microstructure tissue morphology, blood absorption spectral characteristics, and pH contribute to the behavior of the observed signal which may be used to obtain tissue functional information and offer predictive capability.

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Detection of Intestinal Ischemia Using a Microdialysis Technique in an Animal Model

Cited by 41 publications

References 24 publications

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Intraperitoneal Microdialysis as a Monitoring Method in the Intensive Care Unit

Optical spectroscopy approach for the predictive assessment of kidney functional recovery following ischemic injury

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