Detection of Intestinal Protozoa in the Clinical Laboratory

Mchardy, Ian Howard; Wu, Mark Li-cheng; Shimizu-Cohen, Robyn; Couturier, Marc Roger; Humphries, Romney M.

doi:10.1128/jcm.02877-13

Cited by 190 publications

(164 citation statements)

References 34 publications

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“…As a scientific role stool examination by concentration method has had high efficacy than double preparation microscopically (McHardy, 2014). Low variance might be attributed to usage of sedimentation (Formal-ether technique) instead of flotation technique in current study.…”

Section: Resultsmentioning

confidence: 97%

Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province

Salman¹,

Al-Taee²,

Abid³

2016

Int.J.Curr.Microbiol.App.Sci

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Giardiasis is waterborne disease which had impact on public health in anywhere of the world, particularly in communities living under low level of sanitation and poor hygienic condition. Selection of such laboratory methods with high accuracy and precise technique recently had great role in reducing the rate of intestinal parasites in general and Giardia lamblia in particular. So the current study was conducted to fulfill this purpose. Three different microscopic based laboratory techniques namely direct double wet preparation, formal-ether and fecal stained smears by 9 different biological stains were applied for detecting Giardia lamblia stages in a total of 417 stool samples of Internal Iraqi Displaced peoples(IIDPPs) in Kirkuk city. The overall rate of Intestinal parasitic infection was 19.66%(82),,which included 10.31 %(43) as pure giardiasis and 9.35 %(39) for other 9 intestinal parasites. They involve: Blastocyst homonis 4.17 %, Entamoeba histolytica 1.67 %,Cryptosporidium parvum 1.43 %, Entamoeba coli 0.71 %, Cyclospora cayetanensis, 0.49 %, and 0.23 % for each of Entamoeba hartmani, Iodomoeba butschili, Hymenolepis nana and Ancylostoma duodenale. Statistically formalinether technique show high efficacy and significance than wet preparation technique in demonstrating other intestinal parasites. Eighty two positive samples were tested as fecal smears stained by nine different biological stains were examined by microscope. High rates of giardiasis 36.58 %.34.14 %,32.92 % and 25.6 % were recorded by using alcoholic eosin 1%, modified Gram staining+chromotrope 2R, alcoholic aceto-carmine 1 % and calcofluor white stains respectively. Followed by 24.39 %, 23.17 % and 19.15 % for Chromotrope 2R,trichrome stain and modified Ziehl-Neelsen stains respectively. While low rates 10.97 % and 7.31 % were recorded for haematoxyline-Ehrlich and 3 % of giemsa stain respectively. Giardiasis was contributing high rate from intestinal parasites among IIDPs,which had high healthy impact of this group of Iraqi communities. Fecal smears staining methods show high efficacy than direct microscopy and sedimentation technique in detecting and demonstrating Giardia lamblia microscopically, specially 1% of alcoholic eosin, alcoholic aceto-carmine and modified gram staining-hot chromotrope 2R stains. K e y w o r d sGiardiasis, formal-ether,

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Section: Resultsmentioning

confidence: 97%

Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province

Salman¹,

Al-Taee²,

Abid³

2016

Int.J.Curr.Microbiol.App.Sci

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“…Microscopic checking of stool samples for the detection of cysts, oocysts, and trophozoites continues to be the choice of the diagnostic method for many laboratories [55]; however, the method requires technical expertise, and it is laborious; it can also be insensitive when infection is on low levels. Currently, molecular methods, including polymerase chain reaction (PCR), are available for detecting and identifying intestinal parasites of protists, and demonstrate high sensitivity and specificity with respect to conventional methods such as microscopy [56,57].…”

Section: Molecular Identification and Subtype Analysismentioning

confidence: 99%

Genetic diversity and pathogenicity of Blastocystis

Skotarczak¹

2018

Ann Agric Environ Med.

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Blastocystis is a unicellular, anaerobic protist which lives in the intestinal tract of diverse animals, including humans. It was found that the host specificity and the pathogenic potential of different isolates are correlated with sequence variations in the SSU-rRNA gene. Identification of the organism to the species level is still an unresolved challenge. Genetic diversity revisions have led to the identification of 17 subtypes (STs) within the Blastocystis genus, and 9 (ST1 to ST9) have been reported in humans with varying prevalence. Since the members of the genus revealed a large genetic diversity, several molecular modalities of subtyping methods have been developed. Numerous studies on conveying the pathogenic potential to the molecular subtypes are available, but they could not be compared or analysed with the different molecular techniques employed. The use of different approaches may give false positives during diagnosis and the possibility of missed infections. A review of recent scientific literature indicates that the development of PCR assays is needed for molecular epidemiology and for mixed infections in health and disease cohorts, and also to help identify sources of Blastocystis transmission to humans, as well as to identify potential animal and environmental reservoirs. This review summarizes some of the recent progress and improvements in Blastocystis research on genetic diversity, taxonomy, molecular epidemiology, pathogenicity and subtyping methods.

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“…Patients were asked to provide fresh faecal samples. Stool specimens were examined for intestinal parasites using the standard routine methods used by hospitals and microbiological laboratories for diagnosing parasites (15). A direct saline smear preparation was made and examined by light microscopy.…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern Jordan

Jaran¹

2016

East Mediterr Health J

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This study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern Jordan. A total of 21 906 stool samples were collected over a period of 4 years (2009)(2010)(2011)(2012)(2013) from 5 government hospitals in 3 cities. Samples were processed and examined microscopically and by concentration methods. Parasitic infection was found in 9611 samples (44%). Giardia lamblia was the most prevalent parasite (41%) followed by Entamoeba histolytica (31%) and Ent. coli (13%); the least prevalent parasites were Ascaris lumbricoides (1%), Hymenolepis nana, Taenia sp., and Chilomastix mesnili (all < 1%). The prevalence of different parasites varied according to season, on average the summer months showed the highest incidence of parasitic infection (62%) compared with the winter months (16%). Giardia lamblia and Ent. histolytica were most prevalent in the summer months. ‫ـراض‬

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Detection of Intestinal Protozoa in the Clinical Laboratory

Cited by 190 publications

References 34 publications

Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province

Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province

Genetic diversity and pathogenicity of Blastocystis

Prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern Jordan

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