Detection of Irradiated Fresh Chicken, Pork and Fish using the DNA Comet Assay

Cerda, Humberto

doi:10.1006/fstl.1997.0304

Cited by 23 publications

(8 citation statements)

References 6 publications

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“…For irradiated samples, intact cells were not seen in these samples, which suggest that the DNA comet assay could be used to discriminate visually irradiated ostrich meat from the nonirradiated controls at 1 kGy or more. Also, these results are in agreement with a report on DNA comet assay in irradiated fresh chicken, pork, and salmon (Cerda 1998a). Figure 2 shows significant differences in tail length (from the comet head to the end of the tail) between the control and 3 other irradiated samples.…”

Section: Dna Comet Assaysupporting

confidence: 94%

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Detection Properties of Irradiated Ostrich Meat by DNA Comet Assay and Radiation‐induced Hydrocarbons

Chung¹,

Hong²,

Kim³

et al. 2004

Journal of Food Science

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Detection of irradiated ostrich meat with doses of 1 to 10 kGy using the DNA comet assay and hydrocarbon determinations by gas chromatography-mass spectrometry was investigated. The irradiated ostrich meat showed comets with long tails whereas nonirradiated controls showed intact cells only slight comets. Concentrations of radiation-induced hydrocarbons increased with irradiation dose. Radiation-induced hydrocarbons in ostrich meat were detected at doses of 1 kGy or more; however, they were not detected in nonirradiated controls. The prominent radiation-induced hydrocarbons included 14:1, 15:0, 16:2, 16:3, 17:1, and 17:2, with 16:2 the most abundant.

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Section: Dna Comet Assaysupporting

confidence: 94%

“…However, the difference in comet length was not significant among the irradiated samples. Cerda (1998a) reported A, 0 kGy; B, 1 kGy; C, 3 kGy; D, 7 kGy). Silver staining: anode to the right.…”

Section: Dna Comet Assaymentioning

confidence: 96%

Detection Properties of Irradiated Ostrich Meat by DNA Comet Assay and Radiation‐induced Hydrocarbons

Chung¹,

Hong²,

Kim³

et al. 2004

Journal of Food Science

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“…However, double strand DNA damage can also be observed in case the comet assay is used under neutral conditions [25]. For the analysis, the supernatant of testicular tissue homogenate was used [26]. All microscope slides were covered with a coat of agarose (0.5%, normal melting point) and dehydrated at room temperature.…”

Section: Comet Assaymentioning

confidence: 99%

Single-strand DNA breaks and oxidative changes in rat testes exposed to radiofrequency radiation emitted from cellular phones

Alkış

Akdağ

Daşdağ

et al. 2019

Biotechnology & Biotechnological Equipment

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The testes are a sensitive organ to electromagnetic pollution and people are concerned about the harmful effects of the radiofrequency radiation (RFR) emitted from cellular phones. Therefore, the purpose of this study was to investigate the effects of long-term exposure to different RFR frequencies on single-strand DNA breaks and oxidative changes in rat testicular tissue. Twenty-eight male Sprague-Dawley rats were divided randomly into four groups. Three groups were exposed to radiation emitted from 900, 1800 and 2100 MHz RF generators, 2 h/day for 6 months. The sham-control group was kept under the same experimental conditions but the RFR generator was turned off. Immediately after the last exposure, testes were removed and DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were analyzed. The results of this study indicated that RFR increased TOS, OSI, MDA and 8-OHdG (p < 0.05). TAS levels in the exposed group were lower than in the sham group (p < 0.05). In terms of DNA damage, the tail intensities in the comet assay were higher in the exposure groups (p < 0.05). This study demonstrated that long-term exposure to RFR emitted by cellular phones may cause oxidative stress and oxidative DNA damage in rat testicular tissue and may generate DNA single-strand breaks at high frequencies (1800 and 2100 MHz). Our results showed that some RFR emitted from cellular phones has potential to lead to cell damage in the testes.

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“…For comet assay, a piece of the liver cut into thin slices were placed in phosphate buffered saline. Tissues were allowed to stir for dissociation at 500 rpm for 10 min to get the cell suspension [25] . For oxidative stress parameters, a homogenate from about 0.5 g of the remaining liver was obtained with a homogenizer (IKA Ultra-Turrax T10 basic model, Germany) in cold buffer solution of 140 mM KCl and 50 mM Tris-HCl (pH 7.6) for 2 min at 13000 rpm.…”

Section: Sample Preparationmentioning

confidence: 99%

Sıçanlarda Dimetoatla Oluşturulan Hepatotoksisite Üzerine Taflan (Laurocerasus officinalis Roem.) Meyvesinin Koruyucu Etkisi

Eken¹,

Endirlik²,

Baldemir³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleEken A, Ünlü Endirlik B, Bakır E, Baldemir A, Yay AH, Cantürk F: Effect of Laurocerasus officinalis Roem. (Cherry laurel) fruit on dimethoate induced hepatotoxicity in rats. Kafkas Univ Vet Fak Derg, 23 (5): 779-787, 2017779-787, . DOI: 10.9775/kvfd.2017 Abstract Dimethoate is one of the most important organophosphate insecticides and may cause oxidative stress leading to production of free radicals and alterations in antioxidants. The aim of this study was to investigate the protective effects of Laurocerasus officinalis Roem. fruit extract containing antioxidant compounds on dimethoate-induced hepatotoxicity in rats. The rats were divided into six groups as follows: Control group; dimethoate-treated group; L. officinalis-treated group; the group of pre-treatment with L. officinalis prior to dimethoate; the group of pre-treatment with vitamin C before dimethoate; the group of post-treatment with L. officinalis after dimethoate. Aspartate transaminase (AST), alanine transaminase (ALT), and total bilirubin (TBil) as liver function tests and the oxidative stress parameters such as total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were measured. The DNA damage was determined with comet assay. The results indicated that dimethoate caused a significant increase in AST, ALT, TOS, MDA, DNA damage and an important decrease in TAS, SOD, CAT, GPx as compared to control group. However, administration of L. officinalis or vitamin C to dimethoate-exposed rats restored these biochemical and oxidative stress parameters to nearly normal levels. In conclusion, toxic effects of dimethoate on rat liver are mainly attributed to hepatic function enzymes, oxidative stress and DNA damage, while these effects were largely ameliorated by L. officinalis fruit extract. Keywords: Laurocerasus officinalis Roem, Cherry Laurel, Dimethoate, Hepatotoxicity, Rat Sıçanlarda Dimetoatla Oluşturulan Hepatotoksisite Üzerine Taflan (Laurocerasus officinalis Roem.) Meyvesinin Koruyucu Etkisi ÖzetDimetoat en önemli organofosfatlı insektisitlerden biridir, serbest radikallerin üretimine ve antioksidanlarda değişimlere yol açarak oksidatif strese neden olur. Çalışmanın amacı, ratlarda antioksidan bileşikler içeren Laurocerasus officinalis Roem. meyve ekstresinin dimetoat kaynaklı karaciğer toksisitesindeki koruyucu etkilerini incelemektir. Ratlar aşağıdaki gibi altı gruba ayrıldı: Kontrol grubu; dimetoate ile muamele edilen grup; L. officinalis ile muamele edilen grup; dimetoat öncesi L. officinalis verilen grup; dimetoat öncesi vitamin C verilen grup; dimetoat sonrası L. officinalis ile muamele edilen grup. Karaciğer fonksiyon testleri olarak aspartat transaminaz (AST), alanin transaminaz (ALT) ve toplam bilirubin (TBil) ile toplam oksidan durum (TOS), toplam antioksidan durum (TAS), süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve malondialdehit (MDA) gibi oksidatif stres parametreleri ölçüldü. DNA...

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Detection of Irradiated Fresh Chicken, Pork and Fish using the DNA Comet Assay

Cited by 23 publications

References 6 publications

Detection Properties of Irradiated Ostrich Meat by DNA Comet Assay and Radiation‐induced Hydrocarbons

Detection Properties of Irradiated Ostrich Meat by DNA Comet Assay and Radiation‐induced Hydrocarbons

Single-strand DNA breaks and oxidative changes in rat testes exposed to radiofrequency radiation emitted from cellular phones

Sıçanlarda Dimetoatla Oluşturulan Hepatotoksisite Üzerine Taflan (Laurocerasus officinalis Roem.) Meyvesinin Koruyucu Etkisi

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