Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis

Erel, Yakup; Yazıcı, Nizamettin; Özvatan, Sümer; Erçin, Demet; Çetinkaya, Nurcan

doi:10.1016/j.radphyschem.2009.05.023

Cited by 18 publications

(26 citation statements)

References 24 publications

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“…The cell suspension (100 μL) was mixed with 1 mL of low-melting agarose (0.8 % in PBS). A 100 μL of this mixture was spread onto precoated slides (Erel et al, 2009). …”

Section: Preparation Of Single-cell Suspensionmentioning

confidence: 99%

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Güven

Pandır

Baş

2015

Turkish Journal of Entomology

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SummaryExposure to ultraviolet (UV) radiation is hazardous for all organisms. UV shows numerous effects on insects. In this study, mortality effect of UV on Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae) larvae was studied. The larvae were exposed to UV radiation (254 and 365 nm) at different time periods (15, 30, 45 and 60 min). After exposure, mortality rate, comet assay studies, antioxidant enzyme activities (SOD, CAT, GST and GPx), and MDA levels were investigated on the larvae. The mortality rate of E. kuehniella larvae increased with increasing exposure times. It was determined that 254 nm was more effective than 365 nm compared to the effects of short (254 nm) and long-wave (365 nm) radiations on mortality rate of E. kuehniella larvae. Likewise mortality rate, 254 nm had more potency than 365 nm on antioxidant enzyme activities and levels of MDA. According to the comet assay results, the tail DNA% and comet tail length significantly increased in all exposure times at 254 nm, but these changes were seen only 45 min and 60 min at 365 nm of UV radiation. Therefore, tail DNA% and tail lenghts of 254 nm radiation could be greater than the tail lenghts of 365 nm UV radiation. As a result, the UV radiation could be effectively as a safe pest control method and as an alternative to environmentally hazardous chemical pesticides. Key words: Ephestia kuehniella, UV radiation, mortality, antioxidant enzymes, comet assay ÖzetUltraviyole (UV) radyasyona maruz kalmak tüm canlılar için tehlikelidir. UV böcekler üzerinde birçok etki göstermektedir. Bu çalışmada, Ephestia kuehniella 1879 (Lepidoptera: Pyralidae) larvaları üzerine UV'nin öldürücü etkisi çalışıldı. Larvalar UV radyasyonuna (254 ve 365 nm) farklı zaman peryotlarında (15, 30, 45 ve 60 dk.) maruz bırakıldı. Çalışma sonunda larvaların ölüm oranları, komet değeleri ve antioksidan enzim aktiviteleri (SOD, CAT, GST ve GPx) ve MDA seviyeleri araştırıldı. Işınlama süresi arttıkça E. kuehniella larvalarının ölüm oranları artmıştır. Kısa (254 nm) ve uzun (365 nm) dalga boylarını ölüm oranları üzerindeki etkilerini karşılaştırdığımızda 254 nm dalga boyunun 365 nm dalga boyundan daha etkili olduğu belirlenmiştir. Bu durum, ölüm oranı, antioksidan enzim aktivitesi ve MDA seviyesinde de tespit edilmiştir. Komet değerlendirme sonuçlarına göre kuyruk % DNA'sı ve komet kuyruk uzunluğu 254 nm dalga boyutunun tüm uygulama zamanlarında istatistiksel olarak artarken bu değişimler 365 nm UV radyasyonun yalnızca 45. ve 60. dk. larında tespit edilmiştir. Bundan dolayı, kuyruk % DNA'sı ve kuyruk uzunluğu 254 nm dalga boyunda 365 nm dalga boyuna göre daha fazladır. Sonuç olarak, UV radyasyon depo zararlılarının kontrolünde güvenilir bir metod ve çevreye zararlı kimyasal pestisitlere bir alternatif olarak kullanılabilir.Anahtar sözcükler: Ephestia kuehniella, UV radyasyon, ölüm oranı, antioksidan enzimler, komet değerlendirme

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“…The cell suspension (100 μL) was mixed with 1 mL of low-melting agarose (0.8 % in PBS). A 100 μL of this mixture was spread onto precoated slides (Erel et al, 2009). …”

Section: Preparation Of Single-cell Suspensionmentioning

confidence: 99%

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Güven

Pandır

Baş

2015

Turkish Journal of Entomology

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show abstract

“…Cell suspension (100 μL) was mixed with 1 mL of low-melting agarose (0.8% in PBS). A 100 μL of this mixture was spread on precoated slides (Erel et al, 2009). …”

Section: Preparation Of Single-cell Suspensionmentioning

confidence: 99%

Effect of microwave radiation on stored product pest Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Pandır¹,

Güven²

2014

Turkish Journal of Entomology

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This study was designed to examine the effects of microwave radiation exposure on Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) larvae. One to two day old larvae were exposed to microwave radiation at the powers of 70, 150, 300 and 600 W for different exposure times (1-50 s). Mortality ratio in larvae increased significantly with increasing exposure time at all powers of microwave radiation. Completele mortality was achieved at the power of 70 W and the longest exposure time (50 s) Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) in the larvae tissue were evaluated.. SOD, CAT, GPx activities decreased and MDA level increased in the microwave radiation-treated larvae tissue compared to control group at 300 and 600 W (P < 0.05). There was no significant DNA damage detectable at 70, 150 and 300 W for 50 s when compared with control group. However, 50 s of 600 W powers was showed effects on tail length and tail intensity indicating DNA damage.These results indicate that high powers of microwave radiation treatments cause some effects on the stored product pest E. kuehniella larvae.

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“…The Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) method has been used on spices (Choi et al, 2004a;Choi et al, 2004b;Oh et al, 2003), whereas the DNA Comet assay has been applied to a wide range of foods, such as papaya, melon, watermelon and kiwi (Jo and Kwon, 2006;Marin-Huachaca et al, 2004), pork, poultry, beef and other types of meat (Araujo et al, 2004;Khan et al, 2003;Marin-Huachaca et al, 2005;Villavicencio et al, 2004a;Villavicencio et al, 2004b), quail meat and seafood (Erel et al, 2009;Khan et al, 2003), and cereals and nuts (Khan et al, 2005a;Khan et al, 2005b).…”

Section: Biological and Microbiological Screening Methodsmentioning

confidence: 99%

Scientific Opinion on the Chemical Safety of Irradiation of Food

Flavourings¹

2011

EFSA Journal

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Irradiation (gamma rays, electrons or X-rays) is applied to foods for improving food safety and removing and reducing pests that are harmful to plants or plant products. As ionising radiation passes through food, it creates a trail of chemical transformations by primary and secondary radiolysis effects. The main reported radiolytic products are certain hydrocarbons and 2-alkylcyclobutanones produced from the major fatty acids in food, and some cholesterol oxides and furans. Most of these substances are also formed in food that has been subjected to other processing treatments and are thus not exclusively formed by irradiation. Furthermore, the quantities in which they occur in irradiated food are not significantly higher than those being formed in heat treatments. Since the last SCF opinion in 2003, several in vitro genotoxicity studies on irradiated foods have been published. The available data indicate that at least some 2-alkylcyclobutanones may induce DNA damage in vitro. No in vivo genotoxicity studies are available; however, a genotoxic hazard in humans is considered unlikely by the Panel in view of the plausible indirect mechanism underlying the genotoxicity of alkylcyclobutanones in vitro. Concerning other radiolytic products no new relevant toxicological studies have been reported. The weight of evidence from recent literature regarding biological effects supports the food classes and radiation doses specified in previous SCF opinion in 2003. The only new contrary evidence was indicated in publications on leukoencephalomyelopathy in cats which have been fed mainly or exclusively with highly irradiated feed. However a clear mechanistic explanation in terms of risk assessment has not been established. Considering that only a very limited quantity of food is irradiated in Europe currently, the Panel is of the view that there is not an immediate cause for concern. However, the relevance of the cats studies for human health should be clarified.

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Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis

Cited by 18 publications

References 24 publications

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Effect of microwave radiation on stored product pest Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Scientific Opinion on the Chemical Safety of Irradiation of Food

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