Detection of Israeli Acute Paralysis Virus (IAPV) and <i>Apis mellifera</i> Filamentous Virus (AmFV) in Honey Bees in Mexico

García-Anaya, Mayra C.; Romo-Chacón, Alejandro; Sáenz-Mendoza, Alma I.; Pérez-Ordóñez, Gerardo; Acosta-Muñíz, Carlos H.

doi:10.2478/jas-2018-0009

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…A few previous studies had reported the presence of DWV in localities within the states of Chihuahua in the North, Nayarit in the Pacific Coast, and the Federal District in the High Plateau region [28][29][30]68], although its prevalence and intensity were not determined. More recently, a study conducted in the state of Jalisco reported the frequency and levels of V. destructor and DWV in honey bee colonies of African or European descent [69].…”

Section: Discussionmentioning

confidence: 97%

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Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Correa-Benítez,

Anguiano-Baez,

Heneidi-Zeckua

et al. 2023

Animals

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Mexico is a major honey producer, but not much information exists about the health status of honey bees (Apis mellifera L.) in the country. This study was conducted to determine the sanitary status of adult honey bees in Mexico’s five beekeeping regions. Samples from 369 apiaries were diagnosed to identify pathogens such as Varroa destructor, which was quantified, Acarapis woodi, Nosema spp., and five viruses. Colonies were also inspected for the presence of the small hive beetle (SHB), Aethina tumida. Varroa destructor was found in 83.5% of the apiaries, with the Pacific Coast region having the highest prevalence (>95%) and rates (4.5% ± 0.6). Acarapis woodi was detected in only one apiary from the Pacific Coast, whereas Nosema spp. were prevalent in 48.5% of the apiaries, with the highest and lowest frequencies in the Yucatan Peninsula and North regions (64.6% and 10.2%, respectively). For viruses, deformed wing virus (DWV) was detected in 26.1% of the apiaries, with the highest frequency in the Pacific Coast region (44.7%). Israeli acute paralysis virus (IAPV) was diagnosed in 3.2% of the samples and sacbrood bee virus (SBV) in 23.3% of them, with the highest frequency in the High Plateau region (36.4%). Chronic bee paralysis and Kashmir bee viruses were not detected. SHB prevalence was 25.2% nationwide, with the highest frequency in the Yucatan Peninsula (39.2%). This study shows that the most common parasites of adult honey bees in Mexico are V. destructor and Nosema spp., and that the most prevalent virus is DWV, whereas SHB is highly prevalent in the Yucatan Peninsula. This information could be useful to design disease control strategies for honey bee colonies in different regions of Mexico.

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Section: Discussionmentioning

confidence: 97%

“…Another common virus found in brood and adult honey bees is the sacbrood bee virus (SBV), which does not seem to pose a major threat to honey bee health. In Mexico, the presence of DWV, ABPV, IAPV, KBV, and SBV has been reported and confirmed by molecular analyses, but only in some states or localities of the country [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Correa-Benítez,

Anguiano-Baez,

Heneidi-Zeckua

et al. 2023

Animals

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“…Samples were RT-PCR analyzed to detect DWV, BQCV, IAPV, and CBPV. These viruses had been previously reported to exist in Mexico [ 47 , 48 , 49 , 50 ]. Total RNA was extracted with TRIzol ® reagent (Fisher Scientific, Mississauga, ON, Canada) from three bees per sample following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Genotype, but Not Climate, Affects the Resistance of Honey Bees (Apis mellifera) to Viral Infections and to the Mite Varroa destructor

Ramos-Cuellar

Mora

Contreras-Escareño

et al. 2022

Veterinary Sciences

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This study was conducted to analyze the effect of genotype and climate on the resistance of honey bee (Apis mellifera) colonies to parasitic and viral diseases. The prevalence and intensity of parasitism by Varroa destructor, or infection by Nosema spp., and four honey bee viruses were determined in 365 colonies of predominantly European or African ancestry (descendants of A. m. scutellata) in subtropical and temperate regions of Mexico. Varroa destructor was the most prevalent parasite (95%), whilst N. ceranae was the least prevalent parasite (15%). Deformed wing virus (DWV) and black queen cell virus (BQCV) were the only viruses detected, at frequencies of 38% and 66%, respectively. Varroa destructor was significantly more prevalent in colonies of European ancestry (p < 0.05), and the intensity of parasitism by V. destructor or infection by DWV and BQCV was also significantly higher in colonies of European descent than in African descent colonies (p < 0.01), although no genotype–parasite associations were found for N. ceranae. Additionally, significant and positive correlations were found between V. destructor and DWV levels, and the abundance of these pathogens was negatively correlated with the African ancestry of colonies (p < 0.01). However, there were no significant effects of environment on parasitism or infection intensity for the colonies of both genotypes. Therefore, it is concluded that the genotype of honey bee colonies, but not climate, influences their resistance to DWV, BQCV, and V. destructor.

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“…In Israel, IAPV was isolated from dead honey bees, and the characterization of the virus showed that there was a high similarity between this virus and the Kashmir bee virus and ABPV [7]. There are also several reports characterizing IAPV in other countries [10][11][12][13]. Moreover, the results of studies show that IAPV causes a permanent infection at all growth stages and in different honey bee species.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran

2023

ARI

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In this study, the Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, was investigated in honey bee colonies, which had a history of mortality, population decline, and parasitic diseases. Samples (adult honey bees) were collected from 328 apiaries from three provinces (Tehran, Alborz, and Mazandaran) of Iran to detect IAPV. After sample preparation, RNA was extracted and cDNA was synthesized to perform the reverse transcription polymerase chain reaction (RT-PCR) method using a PCR primer pair, and a 185 bp fragment was amplified. The results showed that out of 328 samples, 103 (31.4%) samples were positive, which were from Mazandaran (14.33%), Tehran (8.84%), and Alborz (8.23%) provinces. Subsequently, some of the positive samples were sequenced and a phylogenetic tree was drawn. The phylogenetic tree showed that the virus isolates were divided into two distinct groups, including one group that had a high similarity to the European acute bee paralysis virus (ABPV) and one group that had a high similarity to the Kashmir bee virus. In addition, the sequences of the samples in three regions were separated in a node from the strains of ABPV from Eastern Europe. Since the length of the branch between the Iranian sequences and the different strains of ABPV from Eastern Europe was short, it can be assumed that the sequences from Iran have a common ancestor with the mentioned strains of ABPV from Eastern Europe.

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Detection of Israeli Acute Paralysis Virus (IAPV) and Apis mellifera Filamentous Virus (AmFV) in Honey Bees in Mexico

Cited by 7 publications

References 18 publications

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Prevalence of Adult Honey Bee (Apis mellifera L.) Pests and Pathogens in the Five Beekeeping Regions of Mexico

Genotype, but Not Climate, Affects the Resistance of Honey Bees (Apis mellifera) to Viral Infections and to the Mite Varroa destructor

Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran

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