Detection of Kaposi sarcoma-associated herpesvirus in bone marrow biopsy samples from patients with multiple myeloma

Abstract

BACKGROUND Kaposi sarcoma‐associated herpesvirus (KSHV) recently has been identified in the bone marrow (BM) dendritic cell of multiple myeloma (MM) patients. However, whether or not KSHV is associated with MM remains controversial because many studies have failed to detect the presence of KSHV DNA sequences in the BM of their MM patients. METHODS We have assayed for KSHV DNA sequences in the BM biopsy samples from 49 patients with MM and from 8 patients with normal BM, using nested polymerase chain reaction and dot blot analysis. The polymerase chain reaction product of KSHV was further determined by single‐strand conformation polymorphism and sequence analyses. RESULTS KSHV DNA was detectable in 22 of 49 patients (44.9%) with MM but was not detectable in normal BM cells. Single‐strand conformation polymorphism and sequence analyses showed that there were interpatient specific mutations. Sixteen out of 22 KSHV DNA sequences belonged to a previously defined subgroup, and the other 6 remain unclassified and may represent distinct strains of KSHV in Taiwan. CONCLUSIONS Data strongly supported that KSHV infection did exist in the BM of the current study patients with MM. However, the role of KSHV in the pathogenesis of multiple myeloma remains to be determined. Cancer 2001;91:1409–13. © 2001 American Cancer Society.

Parvovirus B19 infection in Taiwanese patients with hematological disorders

“…The PCR reactions were carried out in a final volume of 50 ml reaction mixture, and amplifications were performed in a Perkin-Elmer 2400 Thermocycler (Applied Biosystems, Foster City, CA); 10-ml aliquots of the N-PCR products were analyzed on 2% agarose gels containing ethidium bromide and were photographed under ultraviolet (UV) light [Kerr et al, 1995]. PCR using b-actin primers was carried out on all samples to standardize the genomic cDNA [Hsu et al, 2001].…”

“…The N-PCR products were transferred onto nitrocellulose filter membranes, which were then hybridized to a [g-32 P] ATP (6,000 Ci/mmol, Amersham) end-labeled 26-bp oligomer probe internal to the nucleotide sequences (5 0 -CAA,GTT, AGC,GTA,CAA,CTA,CCC,GG-T,AC-3 0 ), as previously described [Hsu et al, 2001]. The filter was washed and exposed to Kodak X-O mat XAR film (Eastman Kodak, Rochester, NY) using an intensifying screen.…”

Split genes and their expression in Kaposi's sarcoma‐associated herpesvirus

“…KS is a tumour of endothelial cell origin and MCD is a B-cell lymphoproliferative disorder. Recent reports further suggest that KSHV infection might play a role indirectly in the pathogenesis of multiple myeloma [5][6][7]. Like other gammaherpesvirus such as human B lymphocyte-tropic Epstein-Barr virus (EBV), monkey T lymphocyte-tropic herpesvirus saimiri and murine B lymphocyte-tropic herpesvirus 68, KSHV is another human lymphocyte-tropic virus and usually infects B lymphocytes and establishes latency in the lymphocytes following primary infection.…”

Quantification of Dendritic Cells and Osteoclasts in the Bone Marrow of Patients with Monoclonal Gammopathy

“…Because both osteoclasts and dendritic cells originate from a common origin upon different cytokine profiles, it would be of importance to investigate if changes in cell numbers vary in myeloma and B-cell lymphomas in these altered cytokine network. To our knowledge, only a few studies have tried to assess the pathophysiological links between these two cells types during B-cell monoclonal proliferations [10][11][12][13][14][15]. The present study aimed to identify and quantify bone marrow dendritic cell and osteoclasts in patients with a MGUS, after precise histochemical identification of these two cell populations.…”