Detection of kidney disease biomarkers based on fluorescence technology

Yao, Bangben; Giel, Marie‐Claire; Hong, Yuning

doi:10.1039/d0qm01009j

Cited by 36 publications

(22 citation statements)

References 124 publications

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“…GST can be used as a biomarker for early cancer detection and/or diagnosis. It has been reported that excessive GST in blood and urine specimens is associated with the occurrence of kidney and liver diseases, and the highest expression is in extrahepatic tissues. , Hence, the analysis of GST in genuine samples such as blood and urine is essential and desirable.…”

Section: Results and Discussionmentioning

confidence: 99%

Can Direct-Immersion Aqueous–Aqueous Microextraction Be Achieved When Using a Single-Drop System?

et al. 2022

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For the analysis of biological analytes in complex matrices, it is difficult to achieve extraction of analytes and enrichment in an aqueous−aqueous single-drop microextraction system. In this study, we proposed a pH-dependent polydopamine (PDA)-coated vesicle/Fe 3 O 4 magnetic aqueous−aqueous in a single-drop microreactor (SDMR) for the direct fluorescence detection of glutathione S-transferase (GST), a metabolic enzyme involved with crucial biological processes, in biological samples. After extracting and enriching the GST target from an aqueous−aqueous single-drop interface, the extraction process was conducted rapidly in 6 s in the SDMR system. The GST was first extracted from the sample solution via the GST-Aptamer on the polydopamine-coated vesicle/ Fe 3 O 4 nanospheres (Fe 3 O 4 @PDA@GST-Aptamer). Then, as the pH changed from weakly acidic to weakly alkaline in the SDMR system, the GST and GST-Aptamer were released from Fe 3 O 4 @PDA@GST-Aptamer nanospheres and captured by polydiacetylene vesicles via the capture probe. These changes altered the effective conjugation length and angle of the vesicle trunk, generating a highly enhanced fluorescence signal. This not only achieved the purpose of target enrichment but also reduced interferences posed by matrix effects. The approach can be used for the direct detection of GST in genuine urine and blood without any sample pretreatment. The linear range was 0.005 to 0.5 μg/mL, and the limit of detection was 0.834 ng/mL. The recoveries of GST in genuine blood samples ranged from 90.8 to 108.0% and in urine from 91.6 to 102.8%. The method has the capability of handling complex samples directly by enabling microextraction in an aqueous−aqueous single-drop system.

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Section: Results and Discussionmentioning

confidence: 99%

Can Direct-Immersion Aqueous–Aqueous Microextraction Be Achieved When Using a Single-Drop System?

et al. 2022

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“…Here we have applied sequestration to improve the sensitivity of one biosensor and two bioassays all targeting the protein neutrophil gelatinase-associated lipocalin 52,53 (NGAL): an EAB sensor, a paper-based dipstick assay, and an ELISA. Plasma and urine NGAL levels above various threshold values are indicative of acute kidney damage [54][55][56] , with the relevant reported cut-offs in urine of 2 nM for patients with IgA nephropathy 57 , 5 nM for patients with chronic heart failure 56 , 6 nM infants after cardiac surgery 55 , and 85 nM for acute kidney damage (AKI) in adults 58 .…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays

et al. 2022

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“…However, the techniques often required prelabeled molecular beacons or peptides, which can be time consuming, costly, and difficult to synthesize . To address this deficiency, researchers have developed several self-assembling methodologies by integrating different substrates with signal enhancing reported through noncovalent interactions, including a series of aggregation induced emission luminogens (AIEgens). , These strategies generally offer the additional advantages of a broad concentration range and a high signal-to-noise ratio. − However, these fluorescence-based methods rely on noncovalent interactions that can be affected by reaction medium, ionic strength, surface morphology, and protein foldedness so may introduce large variation among experiments and thus have a relatively low reliability. , Surprisingly, the use of covalent probes for the in situ formation of a sensing system for the detection of trypsin activity has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a β-Arylethenesulfonyl Fluoride-Functionalized AIEgen for Activity-Based Urinary Trypsin Detection

Giel

Zhang

et al. 2022

ACS Appl. Bio Mater.

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Trypsin is one of the most important enzymes of the digestive system produced by the pancreatic acinar cells. Abnormal trypsin activity will affect pancreatic function, resulting in the corresponding pathological changes in the human body. Herein, we present a strategy based on the ensemble of a novel dual warhead probe HPC-ESF and the natural trypsin substrate bovine serum albumin (BSA) for the detection of trypsin activity including in real urine samples. The β-arylethenesulfonyl bearing HPC-ESF is nonemissive when dissolved in aqueous solution but becomes highly fluorescent upon conjugation to BSA through covalent bond formation with nucleophilic amino acids to create the HPC-ESF:BSA sensing system. The HPC-ESF:BSA complex can be hydrolyzed in the presence of trypsin, which results in a distinct fluorescence decrease in correlation with trypsin concentration and thus allows the detection of trypsin. Compared to previous methods, our covalent approach is simple to prepare and highly reliable. Our work will provide a different avenue for researchers to design fluorescent sensors based on a covalent labeling strategy, enriching the small library of functional groups available for such applications.

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Detection of kidney disease biomarkers based on fluorescence technology

Abstract: This review summarizes the recent progress in the development of fluorescent probes and methods for the detection of biomarkers for the diagnosis of kidney diseases.

Cited by 36 publications

References 124 publications

Can Direct-Immersion Aqueous–Aqueous Microextraction Be Achieved When Using a Single-Drop System?

Can Direct-Immersion Aqueous–Aqueous Microextraction Be Achieved When Using a Single-Drop System?

The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays

Synthesis of a β-Arylethenesulfonyl Fluoride-Functionalized AIEgen for Activity-Based Urinary Trypsin Detection

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