Detection of KPC-2-producing Citrobacter freundii isolates in Spain

Gómez-Gil, María Rosa; Paño‐Pardo, José Ramón; Romero-Gómez, María Pilar; Gasior, Mercedes; Lorenzo, María; Quiles, Inmaculada; Mingorance, Jesús

doi:10.1093/jac/dkq352

Cited by 28 publications

(24 citation statements)

References 8 publications

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“…Later, it was reported from different hospitals with increased rates (29,32). A multi-central surveillance study performed at a Turkish university hospital from 2009 to 2013 showed that more than 96% of K. pneumoniae isolates harbored blaOXA-48 carbapenamese (21). In another multi-central study conducted on the patients from different hospitals of Turkey as part of the European survey of carbapenemase producing Enterobacteriaceae (EuSCAPE), the high prevalence (83%) of blaOXA-48 was also confirmed (33).…”

Section: Discussionmentioning

confidence: 99%

“…All carbapenem-resistant K. pneumoniae isolates were tested for the presence of carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM by the multiplex PCR using the primers described previously (20)(21)(22). A bacterial suspension (equal to McFarland 4 turbidity) was prepared in sterile water and boiled for 10 minutes.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The PCR reaction mixture contained 1. conditions were used: initial denaturation at 94°C for 3 minutes, followed by 35 cycles of 94°C for 30 seconds, 55°C for 30 seconds, and 72°C for 1 minute, with a final extension step at 72°C for 7 minutes. The amplified DNA products were analyzed by electrophoresis on a 1.5% agarose gel and the results were evaluated according to the size of each amplicon (20)(21)(22).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

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Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

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Background: Carbapenem-resistant Klebsiella pneumoniae isolates can spread among the hospitalized patients and result in serious infections and increase mortality. Molecular studies provide useful information about resistance mechanisms and crosstransmission among the resistant isolates. Objectives: The current study aimed at investigating phenotypic and molecular characteristics of the carbapenem resistance and clonal relationship among the K. pneumoniae isolates recovered from infection sites and rectal swabs of the patients hospitalized in a tertiary hospital. Methods: Resistance to carbapenems was investigated by disc-diffusion and E-test methods. Modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-combine disc (ECD) methods were performed to determine carbapenemases. Carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM were investigated by multiplex polymerase chain reaction (PCR). Clonal relationship among the isolates was determined by the pulsed-field gel electrophoresis (PFGE). Results: Carbapenem resistance was observed in 93 (5.8%) of the 1605 K. pneumoniae isolates. Majority (n = 66, 71%) of the resistant isolates were recovered from the patients in intensive care units (ICUs). Almost all carbapenem-resistant K. pneumoniae isolates (n = 91, 97.8%) were positive with MHT. Only 6 (6.5%) of the resistant isolates were positive with ECD method. The blaOXA-48 and blaNDM-1 were found in 90.3% (n = 84) and 6.5% (n = 6) of the resistant isolates, respectively. The pulsed-field gel electrophoresis yielded

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Section: Discussionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

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Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

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“…Carbapenem-resistant P. aeruginosa isolates were tested for the carbapenemase genes KPC, IMP, VIM, OXA, and NDM by multiplex PCR as previously described [7,[10][11][12]. In order to check the efficiency of the boiling methods for DNA isolation, each multiplex PCR tube also included the primers specific for the ribosomal rpsL housekeeping gene.…”

Section: Detection Of Carbapenemase-encoding Genesmentioning

confidence: 99%

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Müderris

Durmaz

Özdem

et al. 2018

J Infect Dev Ctries

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Introduction: In recent years, the prevalence of multidrug-resistant P. aeruginosa has remarkably increased. Thus, we wanted to investigate the carbapenem resistance mechanisms and clonal relationship among 80 carbapenem-resistant P. aeruginosa strains. Methodology: Carbapenemase production was detected using the Modified Hodge Test (MHT), EDTA combined disc method (ECD), and PCR. Expression levels of efflux and porin genes were mesured by real-time reverse transcription PCR. Clonal relationship of the isolates was investigated by pulsed-field gel electrophoresis (PFGE). Results: Carbapenemase production was detected in 7.5% of the isolates with MHT/ECD tests and in 11.3% of the isolates with PCR. Although the specificity of MHT/ECD was high, the sensitivitivity was low. oprD downregulation and mexB, mexY, and mexD overexpression were demonstrated in 55%, 16.3%, 2.5%, and 2.5% of the isolates, respectively. Multiple carbapenem resistance mechanisms were found in nearly a quarter of the isolates. PFGE typing of the 80 P. aeruginosa isolates yielded 61 different patterns. A total of 29 isolates (36.3%) were classified in 10 clusters, containing 2 to 7 strains. We could not find a strict relationship between PFGE profile and carbapenem resistance mechanisms. Conclusions: Although oprD downregulation and MexAB-OprM overexpression were the most common mechanisms, carbapenem resistance was associated with multiple mechanisms in the study. MHT/ECD tests should not be used alone for investigation of carbapenemase production in P. aeruginosa. Rapid tests with high sensitivity and specificity should be developed for the detection of carbapenemase production in P. aeruginosa.

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“…The most clinically important carbapenemases produced by Enterobacteriaceae are the class B metallo-␤-lactamases (MBLs), represented by VIM, IMP, and NDM types, the class A enzymes of the KPC type, and the class D enzymes, represented by the OXA-48 type (3). In Spain, the number of reports on carbapenemase-producing Enterobacteriaceae (CPE) has increased in recent years (4)(5)(6)(7)(8)(9)(10). However, comprehensive assessment of the impact of CPE in Spain is still missing.…”

mentioning

confidence: 99%

Carbapenemase-Producing Enterobacteriaceae in Spain in 2012

Oteo

Sáez

Bautista

et al. 2013

Antimicrob Agents Chemother

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We report the epidemiological impact of carbapenemase-producing Enterobacteriaceae (CPE) in Spain in 2012. Of the 237 carbapenemases detected, 163 were from the OXA-48 group, 60 were from VIM-1, 8 were from KPC-2, 5 were from IMP, and 1 was from NDM-1. Interhospital spread of carbapenemase-producing Klebsiella pneumoniae was due to a limited number of multilocus sequence types (MLST) and carbapenemase types, including ST15-VIM-1, ST11-OXA-48, ST405-OXA-48, ST101-KPC-2, and ST11-VIM-1. The number of CPE cases in Spain has increased sharply in recent years, due mainly to the emergence of OXA-48.

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Detection of KPC-2-producing Citrobacter freundii isolates in Spain

Cited by 28 publications

References 8 publications

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Carbapenemase-Producing Enterobacteriaceae in Spain in 2012

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