Detection of LD body in Peripheral Blood Buffy-coat from Suspected Kala-azar Cases of Bangladesh

Roy, Chandan Kumar; Sariullah, Sofia Andalib; Saleh, Ahmed Abu; Miah, Ruhul Amin

doi:10.3329/bjmm.v3i2.5324

Cited by 2 publications

(2 citation statements)

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“…The study found a positivity rate of 31% by buffy coat smear among 67 clinically suspected VL patients. Unfortunately the results of present study cannot be compared with the results of the study by Roy et al (18), because they did not report the buffy coat smear positivity rates among their confirmed VL cases (44/67) and also among those who had been positive by the rK39 rapid test (57/67). Nevertheless, the low positivity rate for buffy coat smear found by Roy et al might be due to the conventional method for preparation of buffy coat smears which they used.…”

Section: Discussioncontrasting

confidence: 55%

“…The study by Sharma et al and our unpublished data indicated that the buffy coat smear should be a good diagnostic method for active VL, since parasitemia among asymptomatic VL patients and healthy controls from areas of endemicity was very low (0% to 1.3%). So far, only one study conducted in Bangladesh has tried to investigate the diagnostic sensitivity and specificity of buffy coat smear for diagnosis of VL (18). The study found a positivity rate of 31% by buffy coat smear among 67 clinically suspected VL patients.…”

Section: Discussionmentioning

confidence: 99%

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Peripheral Blood Buffy Coat Smear: a Promising Tool for Diagnosis of Visceral Leishmaniasis

et al. 2012

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Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor people in the regions of endemicity. Buffy coat smear microscopy, as a minimally invasive, simple alternative for the parasitological diagnosis of VL, was evaluated in this prospective study. One hundred twelve VL patients were enrolled in this study. The buffy coat was separated from peripheral blood of all enrolled subjects using Histopaque-1119 solution. Leishman-stained buffy coat smears were examined for Leishmania donovani bodies, and buffy coat was also utilized for detection of parasite DNA by Leishmania nested PCR (LnPCR) for all cases. Concomitant splenic smears could be examined for L. donovani bodies in 66 cases, and the parasite load was graded on a scale of 1؉ to 6؉ for L. donovani-positive smears. All splenic smear-positive cases were also found to be positive by LnPCR. Of 112 enrolled VL cases, 103 (92%) were found to be positive for L. donovani bodies in buffy coat smear microscopy, which is promising as a confirmative diagnosis tool. We have also found a significant association of the buffy coat smear positivity with parasitic burden in the spleen smear. In this preliminary observation in Bangladesh, buffy coat smear microscopy has been found to be very simple, minimally invasive, and risk-free method of parasitological diagnosis of VL with a good diagnostic accuracy and potential for field use.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Peripheral Blood Buffy Coat Smear: a Promising Tool for Diagnosis of Visceral Leishmaniasis

et al. 2012

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Risk-free point-of-care visceral leishmaniasis diagnostics: combining buffy coat microscopy and immunoassay in tertiary rural hospitals in Sudan

2020

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Detection of LD body in Peripheral Blood Buffy-coat from Suspected Kala-azar Cases of Bangladesh

Abstract: The present study has been carried out in an attempt to observe the usefulness of peripheral blood buffy-coat microscopic examination for detection of LD-body from clinically suspected Kala-azar cases.

Cited by 2 publications

References 5 publications

Peripheral Blood Buffy Coat Smear: a Promising Tool for Diagnosis of Visceral Leishmaniasis

Peripheral Blood Buffy Coat Smear: a Promising Tool for Diagnosis of Visceral Leishmaniasis

Risk-free point-of-care visceral leishmaniasis diagnostics: combining buffy coat microscopy and immunoassay in tertiary rural hospitals in Sudan

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