Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification

Li, Wenying; Yang, Yue; Chen, Jian; Zhang, Qingfeng; Wang, Yan; Wang, Fangyuan; Yu, Cong

doi:10.1016/j.bios.2013.09.055

Cited by 58 publications

(26 citation statements)

References 41 publications

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“…With a large dynamic range (6 orders of magnitude), a detection limit down to 30 fM was obtained based on three times standard deviation of the blank, which is at least 500-fold lower than those of previously reported fluorescence methods. 22,[26][27][28][29] This sensitivity is also much higher than those of several electrochemical and electrochemiluminescence approaches 24,25,30,31 and is even comparable to the surface plasmon resonance-based biosensor. 32 The details are summarized in Table 1.…”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 81%

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

et al. 2014

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Herein, we propose a novel and universal biosensing platform based on a polymerase-nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). This platform tactfully integrates two signal amplification modules, strand displacement amplification (SDA) and nicking enzyme signal amplification (NESA), into a one-step system. A bifunctional DNA probe with a stem-loop structure was designed to be partly complementary to the SDA product and digested substrate of NESA for bridging SDA and NESA. ESQM can be performed by using only the enzymes and buffer involved in the SDA module. In the presence of a target, this DNA machine is activated to afford a high quadratic amplified signal. Using Pb 2+ and DNA adenine methylation (Dam) methyltransferase (MTase) as analytes, a sensitive biosensing platform is demonstrated. Low detection limits of 30 fM Pb 2+ and 0.05 U ml − 1 Dam MTase were achieved within a short assay time (40 min), which were each superior to those of most previously reported methods. This DNA machine exhibited high selectivity for Pb 2+ . Furthermore, the successful detection of complex environmental water samples demonstrated the applicability of the proposed strategy in real samples, holding great potential for its application in environmental monitoring, biomedical research and clinical diagnosis. INTRODUCTIONSignal amplification technologies have a critical role in molecular biology research, environmental and food monitoring, forensic identification and clinical diagnosis. Since its creation in 1985, polymerase chain reaction has become the most well-known and widely used amplification technique. 1 However, this technique suffers from an intrinsic drawback, the requirement of precision thermal cycling between three different temperatures in a costly thermal cycler, limiting its popularization and application in point-of-care testing. On the other hand, various alternative isothermal amplification methods, such as rolling circle amplification (RCA), 2,3 strand displacement amplification (SDA), 4 loop-mediated isothermal amplification, 5,6 nicking enzyme signal amplification (NESA) 7-9 and the exponential amplification reaction (EXPAR) 10 have been proposed in the past two decades. These isothermal methods have been developed mainly based on some new findings in molecular biology concerning DNA synthesis and some accessory proteins together with their mimicking in vitro for nucleic acid amplification. Among these methods, EXPAR has been widely used, which is attributed to its rapid amplification kinetics (several minutes) and exceedingly high amplification efficiency (10 6 -to 10 9 -fold). For example, Ye's group 11 and Zhang's group 12,13 have proposed several interesting methods for the highly sensitive detection of microRNA and protein based on EXPAR, respectively. Nevertheless, the EXPAR-based methods involve the weaknesses of early phase non-specific background amplification resulting from the binding of the polymerase to the single-stranded template. 14 Conversely, the linear amplification ...

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Section: Optimization Of Sensing Conditionsmentioning

confidence: 81%

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

et al. 2014

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“…Notably, this large sample volume improved the detection sensitivity by up to 50-fold as a result of the concentration of Pb 2+ ions onto the MCEM from a very dilute aqueous solution. Although this LOD is not comparable with that obtainable using ICP-MS, it is at least one order of magnitude lower than those provided by optical and electrochemical sensors for Pb 2+ ions [49][50][51][52][53][54][55][56][57][58][59][60]. In addition, the detection of Pb 2+ by MCEM/LDI-MS is relatively simple, rapid and high-throughput when compared to ICP-MS.…”

Section: Membrane-based Ldi-ms For Analysis Of Pb 2+ Ionsmentioning

confidence: 72%

“…Therefore, our MCEM/LDI-MS sensing system is a practical tool for the determination of Pb 2+ ions in complicated biological samples. Notably, most probes based on organic chemosensors or NPs are rarely applied for the determination of Pb 2+ ions in such complex biological samples [49][50][51][52][53][54][55][56][57][58][59][60].…”

Section: Practicalitymentioning

confidence: 99%

Membrane-based detection of lead ions in seawater, urine and drinking straws through laser desorption/ionization

Unnikrishnan

Huang

2014

Sensors and Actuators B: Chemical

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“…DNAzymes, a series of functional oligonucleotides, are able to catalyze several types of reactions and are highly special for metal cofactors such as Pb 2 þ ions (Li et al, 2014), Mg 2 þ ions , UO 2 2 þ ions (Liu et al, 2007), and Zn 2 þ ions (Qian et al, 2014), etc. DNAzymes are stable, low cost, and have selectivities that are comparable with that of antibodies (Li et al, 2000), thus exhibiting strong competitiveness in sensing applications (Willner et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Triple functional DNA–protein conjugates: Signal probes for Pb2+ using evanescent wave-induced emission

Wang

Zhou

Shi

2015

Biosensors and Bioelectronics

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Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification

Cited by 58 publications

References 41 publications

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

Polymerase/nicking enzyme synergetic isothermal quadratic DNA machine and its application for one-step amplified biosensing of lead (II) ions at femtomole level and DNA methyltransferase

Membrane-based detection of lead ions in seawater, urine and drinking straws through laser desorption/ionization

Triple functional DNA–protein conjugates: Signal probes for Pb2+ using evanescent wave-induced emission

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