Detection of Legionella with polymerase chain reaction and gene probe methods

Mahbubani, Meena H.; Bej, Asim K.; Miller, Richard D.; Haff, Lawrence A.; DiCesare, Joseph L.; Atlas, Ronald M.

doi:10.1016/0890-8508(90)90051-z

Cited by 138 publications

(97 citation statements)

References 27 publications

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“…A number of PCR-based assays have been developed for the detection and quantification of Legionella in water mainly using the 5S and 16S rRNA genes and the macrophage infectivity potentiator (mip) gene of L. pneumophila [23][24][25][26][27][28][29][30][31]. However, these assays lack the ability to discriminate between living and dead (non-infectious) Legionella cells.…”

Section: Introductionmentioning

confidence: 99%

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Grúas

Alvarez²,

Lara

et al. 2013

Indian J Microbiol

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Rapid and more sensitive methods for the detection and quantification of viable Legionella cells have been developed. In this paper, a comparative analysis of environmental water samples using the ScanVIT-Legionella TM method and the traditional ''gold standard'' method of culturing is realised indicating the usefulness of the ScanVIT method. The ScanVIT-Legionella TM method was performed on environmental water samples from different locations of Huesca region (Spain). Legionella micro-colonies should appear green colour and Legionella pneumophila micro-colonies appear red. Twenty-one environmental water samples analysed by standard culture plus five control samples (Two sterile water samples with Legionella as positive controls and three sterile water samples as negative controls). All of them were used to apply ScanVIT-Legionella TM method. From of 21 environmental samples eleven were positive, six negative with both methods and four samples were negative for culture method and positive for ScanVIT-Legionella TM method. The positive control samples were positive and the negative were negative for both methods. A comparative analysis of the results obtained with two methods showed a strong positive determination coefficient (R 2 = 0.99753). The results demonstrate the usefulness of the ScanVITLegionella TM method as a rapid diagnostic tool in order to provide a diagnosis as quick as possible. ScanVITLegionella TM method offers a series of advantages such as quickly diagnosis, higher sensitivity and the possibility to identify Legionella spp. and L. pneumophila simultaneously.

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Section: Introductionmentioning

confidence: 99%

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Grúas

Alvarez²,

Lara

et al. 2013

Indian J Microbiol

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“…The 5S rRNA primer-probe set was constructed to allow detection of all common Legionella species (Legionella genus LC-PCR). Primers for the latter assay were slightly truncated versions of the 20-mer sequences, L5SL9 and L5SR93, previously reported by Mahbubani et al (35). Due to constraints imposed by the size and sequence of the 5S amplicon, only a single probe, a 23-bp portion of the 50-mer probe, also reported by Mahbubani et al (35) was used.…”

Section: Control Organismsmentioning

confidence: 99%

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

et al. 2001

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“…All suspected Legionella colonies were spread onto GVPC media and sheep blood agar (Nippon Becton Dickinson Co., Tokyo, Japan) media incubated at 35°C, for 1 week, respectively. Colonies grown on GVPC media but not on sheep blood agar media were subjected to further polymerase chain reaction (PCR) examination 35,36 to confirm the genus. In examining the bactericidal activities of the ceramic microbeads against Legionella in 100 mL of rainwater with addition of Legionella suspensions, almost exactly similar procedures as described earlier were employed.…”

Section: Collection Of Rainwater Samples Investigatedmentioning

confidence: 99%

Applicability assessment of ceramic microbeads coated with hydroxyapatite-binding silver/titanium dioxide ceramic composite earthplus™ to the eradication of Legionella in rainwater storage tanks for household use

Oana

Kobayashi

Yamaki

et al. 2015

IJN

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Water environments appear to be the habitats of Legionella species. Legionellosis is considered as a preventable illness because bacterial reservoirs can be controlled and removed. Roof-harvested rainwater has attracted significant attention not only as a groundwater recharge but also as a potential alternative source of nonpotable water. We successfully developed ceramic microbeads coated with hydroxyapatite-binding silver/titanium dioxide ceramic composite earthplus™ using the thermal spraying method. The ceramic microbeads were demonstrated to have bactericidal activities against not only Legionella but also coliform and heterotrophic bacteria. Immersing the ceramic microbeads in household rainwater storage tanks was demonstrated to yield the favorable eradication of Legionella organisms. Not only rapid-acting but also long-lasting bactericidal activities of the ceramic microbead were exhibited against Legionella pneumophila . However, time-dependent attenuation of the bactericidal activities against Legionella were also noted in the sustainability appraisal experiment. Therefore, the problems to be overcome surely remain in constantly managing the Legionella -pollution by means of immersing the ceramic microbeads. The results of our investigation apparently indicate that the earthplus™-coated ceramic microbeads would become the favorable tool for Legionella measures in household rainwater storage tanks, which may become the natural reservoir for Legionella species. Our investigation would justify further research and data collection to obtain more reliable procedures to microbiologically regulate the Legionella in rainwater storage tanks.

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Detection of Legionella with polymerase chain reaction and gene probe methods

Cited by 138 publications

References 27 publications

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

Applicability assessment of ceramic microbeads coated with hydroxyapatite-binding silver/titanium dioxide ceramic composite earthplus™ to the eradication of Legionella in rainwater storage tanks for household use

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Detection of Legionella with polymerase chain reaction and gene probe methods

Cited by 138 publications

References 27 publications

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

Applicability assessment of ceramic microbeads coated with hydroxyapatite-binding silver/titanium dioxide ceramic composite earthplus&trade; to the eradication of Legionella in rainwater storage tanks for household use

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Product

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Applicability assessment of ceramic microbeads coated with hydroxyapatite-binding silver/titanium dioxide ceramic composite earthplus™ to the eradication of Legionella in rainwater storage tanks for household use