Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil

Silva, Elaine A.; Andreotti, Renato; Dias, Edelberto Santos; Barros, Jacqueline Cavalcante; Brazuna, Julia Cristina Maksoud

doi:10.1016/j.exppara.2008.03.011

Cited by 38 publications

(38 citation statements)

References 29 publications

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“…This scenario is similar to those described in recently developed foci that formed during the dispersion of VL to the southern cities of South America through Brazil (Silva et al 2008) and Paraguay (Cousiño 2006) prior to reaching this southernmost focus in Argentina. The similarities between these foci validate the importance of designing regional control strategies.…”

Section: Discussionsupporting

confidence: 57%

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Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Acardi

Liotta

Santini

et al. 2010

Mem. Inst. Oswaldo Cruz

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In this study, a genotypification of Leishmaniawas performed using polimerase chain reaction-restriction fragment length polymorfism (PCR-RFLP) and sequencing techniques to identify species of Leishmaniaparasites in phlebotomine sand flies and dogs naturally infected. Between January-February of 2009, CDC light traps were used to collect insect samples from 13 capture sites in the municipality of Posadas, which is located in the province of Misiones of Argentina. Sand flies identified as Lutzomyia longipalpiswere grouped into 28 separate pools for molecular biological analysis. Canine samples were taken from lymph node aspirates of two symptomatic stray animals that had been positively diagnosed with canine visceral leishmaniasis. One vector pool of 10 sand flies (1 out of the 28 pools tested) and both of the canine samples tested positively for Leishmania infantumby PCR and RFLP analysis. PCR products were confirmed by sequencing and showed a maximum identity with L. infantum. Given that infection was detected in one out of the 28 pools and that at least one infected insect was infected, it was possible to infer an infection rate at least of 0.47% for Lu. longipalpisamong the analyzed samples. These results contribute to incriminate Lu. longipalpis as the vector of L. infantumin the municipality of Posadas, where cases of the disease in humans and dogs have been reported since 2005

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Section: Discussionsupporting

confidence: 57%

“…Grande and Corumbá, it reached 1.9% for Leishmania spp (Silva et al 2008) and 1.5% for the Lu. longipalpis sibling species (and proven vector of L. infantum) Lutzomyia cruzi (de Pita-Pereira et al 2008), respectively.…”

Section: Resultsmentioning

confidence: 99%

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Acardi

Liotta

Santini

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…The risk of transmission is highest from December to April, the wet period associated with an increased sandfly population 6,20 . Weather data for Campo Grande from 2002-2009 are typical of a tropical climate, with temperatures and rainfall rates favorable for the development of the VL vector 8 .…”

Section: Ethical Considerationsmentioning

confidence: 99%

Profile and geographic distribution of reported cases of visceral leishmaniasis in Campo Grande, State of Mato Grosso do Sul, Brazil, from 2002 to 2009

Brazuna

Silva

Brazuna³

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: This study sought to describe the profile and geographic distribution of reported cases of visceral leishmaniasis (VL) in the City of Campo Grande, State of Mato Grosso do Sul (MS), Brazil, from 2002 to 2009. METHODS: Human data were collected from the Brazilian National Information System for Notifiable Diseases. Canine cases and entomological data were obtained from the Information Service for Canine Visceral Leishmaniasis Control/Campo Grande, MS. RESULTS: A total of 951 records from 2002 to 2009 were investigated. The number of reported cases of VL in males was significantly higher (p < 0.0001) than that in females. The higher frequency observed among males was associated with age (p < 0.0001), which increased in individuals aged 40 years and older. The overall fatality rate was 7.4%. Entomological surveys conducted in 2006, 2007, and 2009 showed the insect vector Lutzomyia longipalpis to be present in all urban regions of the county. CONCLUSIONS: VL cases in humans and dogs, as well as in vectors, occurs in all urban regions of Campo Grande. Despite not observing tendencies of increase or reduction in the incidence of the disease due to aging, the major incidence in men is higher in those aged 40 years or above.

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“…Leishmaniasis is an important zoonosis whose cycle is classically characterized by the presence of dipterous vectors of the genus Lutzomyia (GALATI et al, 2003;KAMHAWI, 2006;SILVA et al, 2008). However, entomological studies carried out in different Brazilian municipalities did not confirm the presence of this phlebotomine in areas endemic to L. chagasi (DANTAS-TORRES, 2006).…”

Section: Resultsmentioning

confidence: 99%

Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

Coelho

Bresciani

2013

Rev. Bras. Parasitol. Vet.

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Leishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about the existence of new vectors in the cycle of leishmaniasis. The aim of this paper is to report the molecular detection of Leishmania spp. in a horse fly of the species Tabanus importunus which parasitized an oligosymptomatic dog infected with Leishmania spp. Molecular amplification of the protozoan's DNA in the head, thoracic region and abdomen of the tabanid tested positive for Leishmania complex. This is the first report of the presence of DNA from Leishmania spp. in dipterous insects of the species T. importunus.Keywords: Dogs, canine visceral leishmaniasis, horse fly, PCR, protozoan, vectors. ResumoA leishmaniose é uma importante zoonose, de caráter crônico, causada por protozoários do gênero Leishmania spp. Esta protozoose tem como principal vetor os flebotomíneos, sendo que, no Brasil, o Lutzomyia longipalpis é a principal espécie incriminada na transmissão da leishmaniose Visceral Americana. A presença do ácido desoxirribonucleico (DNA) do parasito em ectoparasitos, como carrapatos e pulgas, tem gerado especulações quanto a existência de novos vetores no ciclo da leishmaniose. Foi objetivo deste estudo relatar a detecção molecular de Leishmania spp. em uma mutuca da espécie Tabanus importunus que parasitava um cão oligossintomático infectado por Leishmania spp. A análise molecular amplificou o DNA do protozoário na cabeça, na região torácica e no abdomen do tabanídeo, resultando como positivo para complexo Leishmania. Este é o primeiro relato da presença de DNA de Leishmania spp. em insetos dipteros da espécie T. importunus.

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Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil

Cited by 38 publications

References 29 publications

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay

Profile and geographic distribution of reported cases of visceral leishmaniasis in Campo Grande, State of Mato Grosso do Sul, Brazil, from 2002 to 2009

Molecular and parasitological detection ofLeishmania spp. in a dipteran of the speciesTabanus importunus

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