Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR

Benassi, Júlia Cristina; Benvenga, Graziella Ulbricht; Ferreira, Helena Lage; Pereira, Vanessa Figueredo; Keid, Lara Borges; Soares, Rodrigo Martins; Oliveira, Trícia Maria Ferreira de Sousa

doi:10.1016/j.exppara.2017.04.004

Cited by 44 publications

(46 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In feline epidemiological investigations most studies were performed on EDTA-blood and usually a lower positivity rate was found in cats compared to dogs from the same area Pennisi et al, 2015a). Limited data are available about other tissues (lymph node, bone marrow, skin) or noninvasive sampling (conjunctival or oral swabs) (Benassi et al, 2017;Brianti et al, 2017;Otranto et al, 2017;Pennisi et al, 2015a). Biopsied skin or mucosal lesions and bone marrow or lymph node samplings obtained from sick cats have usually high parasite loads measured by quantitative real time PCR (Migliazzo et al, 2015) as seen in dogs.…”

Section: Diagnosismentioning

confidence: 99%

Feline leishmaniosis: Is the cat a small dog?

Pennisi¹,

Persichetti

2018

Veterinary Parasitology

135

View full text Add to dashboard Cite

Leishmania infantum is a vector-borne zoonotic disease transmitted by phlebotomine sand flies and dogs are considered the main reservoir of the parasite. Feline leishmaniosis (FeL) caused by L. infantum is an emergent feline disease more and more frequently reported in endemic areas. This review summarizes current knowledge focusing similarities and differences with canine leishmaniosis (CanL). Cats are infected by the same Leishmania species than dogs but prevalence of the infection is lower and cases of disease are less frequently reported. Scarce information is available on adaptive immune response of cats naturally exposed to L. infantum infection and mechanisms responsible for susceptibility or resistance of feline hosts. However, about half of clinical cases of FeL are reported in cats with possible impaired immunocompetence. Coinfections or comorbidities are frequently detected in sick cats and they can contribute to a misrepresentation of clinical FeL albeit lesions associated with the presence of the parasite have been detected in skin, lymph nodes, spleen, bone marrow, liver, oral mucosa, stomach, large bowel, kidney, nasal exudate, lung, eye. As for dogs, skin or mucocutaneous lesions are the most common reason for veterinary consultation and finding on physical examination in cats with leishmaniosis. Molecular investigations of Leishmania DNA and anti- Leishmania antibody detection are largely used with the same methodologies for both CanL and FeL, however few information is available about their diagnostic performance in feline hosts. Treatment of cats with clinical FeL is still empirically based and off label by using the most common drugs prescribed to dogs. Life expectancy of cats with clinical FeL is usually good unless concurrent conditions or complications occur and prognosis does not seem significantly influenced by therapy or retroviral coinfection. According to current knowledge, cats can play a role as additional reservoir host of L. infantum and, in a « One Health » perspective, preventative measures should be taken. In conclusion, albeit feline infection and the associated cat disease caused by L. infantum is increasingly reported in endemic areas and have many similarities with CanL, consolidated evidence-based knowledge is not available and we cannot exclude that important differences between dogs and cats exist about transmission, immunopathogenesis and best practice for management and prevention.

show abstract

Section: Diagnosismentioning

confidence: 99%

Feline leishmaniosis: Is the cat a small dog?

Pennisi¹,

Persichetti

2018

Veterinary Parasitology

135

View full text Add to dashboard Cite

show abstract

“…Ainda é incerta a participação dos gatos na epidemiologia da leishmaniose, sugere-se que eles possam ser reservatórios em regiões endêmicas (Oliveira et al, 2015;Benassi et al, 2017). Os sinais clínicos mais comuns além de hiporexia e consequente emagrecimento as anormalidades clinicopatológicas compatíveis com leishmaniose felina, incluem linfadenomegalia, lesões cutâneas ulcerativas, esfoliativas, dermatite nodular (principalmente na cabeça ou distal membros), lesões oculares (principalmente uveíte), síndrome da gengivo-estomatite crônica felina, úlcera muco cutânea, hipergamaglobulinemia e anemia normocromática normocítica.…”

Section: Discussionunclassified

Fratura Apical De Sesamóide Proximal Em Equino: Relatos De Caso

Borgo¹,

Tosi²,

Depes³

et al. 2019

Estudos Em Patologia Veterinária

View full text Add to dashboard Cite

Revisão: Os Autores O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Conselho Editorial Ciências Humanas e Sociais Aplicadas APRESENTAÇÃO A Patologia Veterinária é uma área da Medicina Veterinária responsável pelo diagnóstico das doenças nos animais domésticos e selvagens, através do exame clínico do animal, dos tecidos e fluidos corporais. É dividida em dois ramos, a patologia da anatomia e a patologia clínica, ambas realizam o diagnóstico de doenças nos animais verificando se há risco para os humanos manusearem, consumirem ou conviverem com estes, sejam eles animais produtores de alimentos, animais selvagens ou exóticos, ou animais de companhia. Além do diagnóstico os veterinários patologistas têm um papel importante na descoberta de novas formas de tratamento, bem como a investigação científica de doenças pré-existentes, ou descobrindo uma nova doença. Para tanto o conhecimento da fisiologia animal é importante, e desta forma conhecer o que está alterado nos estados patológicos. Já o conhecimento das patologias deve ser constantemente aprofundado, através de estudos, leituras, cursos e especializações. Desta forma a Editora Atena apresenta o livro Estudos em Patologia Veterinária, o qual traz estudos de patologia de cães, gatos, bovinos, equinos, pinguins, lambaris, mamíferos selvagens e coelhos.

show abstract

“…Leishmania DNA can be detected and quantified by qPCR in the lacrimal glands of symptomatic dogs [263]. All these clues have prompted testing the efficiency of the conjunctival swab for CVL diagnosis [264][265][266][267] and tracking asymptomatic dog infections [268] but also for diagnosing feline leishmaniasis [267,269,270]. In addition, the detection of Leishmania DNA in conjunctival swabs has also been applied to track L. infantum wild reservoirs [271,272].…”

Section: Conjunctival Swab/lacrimal Fluid/occularmentioning

confidence: 99%

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Sereno

Akhoundi

Sayehmri

et al. 2020

IJMS

View full text Add to dashboard Cite

Unicellular eukaryotes of the Trypanosomatidae family include human and animal pathogens that belong to the Trypanosoma and Leishmania genera. Diagnosis of the diseases they cause requires the sampling of body fluids (e.g., blood, lymph, peritoneal fluid, cerebrospinal fluid) or organ biopsies (e.g., bone marrow, spleen), which are mostly obtained through invasive methods. Body fluids or appendages can be alternatives to these invasive biopsies but appropriateness remains poorly studied. To further address this question, we perform a systematic review on clues evidencing the presence of parasites, genetic material, antibodies, and antigens in body secretions, appendages, or the organs or proximal tissues that produce these materials. Paper selection was based on searches in PubMed, Web of Science, WorldWideScience, SciELO, Embase, and Google. The information of each selected article (n = 333) was classified into different sections and data were extracted from 77 papers. The presence of Trypanosomatidae parasites has been tracked in most of organs or proximal tissues that produce body secretions or appendages, in naturally or experimentally infected hosts. The meta-analysis highlights the paucity of studies on human African trypanosomiasis and an absence on animal trypanosomiasis. Among the collected data high heterogeneity in terms of the I 2 statistic (100%) is recorded. A high positivity is recorded for antibody and genetic material detection in urine of patients and dogs suffering leishmaniasis, and of antigens for leishmaniasis and Chagas disease. Data on conjunctival swabs can be analyzed with molecular methods solely for dogs suffering canine visceral leishmaniasis. Saliva and hair/bristles showed a pretty good positivity that support their potential to be used for leishmaniasis diagnosis. In conclusion, our study pinpoints significant gaps that need to be filled in order to properly address the interest of body secretion and hair or bristles for the diagnosis of infections caused by Leishmania and by other Trypanosomatidae parasites.

show abstract

Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR

Cited by 44 publications

References 32 publications

Feline leishmaniosis: Is the cat a small dog?

Feline leishmaniosis: Is the cat a small dog?

Fratura Apical De Sesamóide Proximal Em Equino: Relatos De Caso

Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis

Contact Info

Product

Resources

About