Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

Guo, Dan; Gan, Zhen-Fei; Jiang, Lei; Cao, Maofeng; Patrice, Fato Tano; Hafez, Mahmoud Elsayed; Li, Dawei

doi:10.1039/c8an02182a

Cited by 8 publications

(3 citation statements)

References 47 publications

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“…By simply treating APN with bestatin and then probe APN ‐ CL , CL images were obtained. As shown in Figure 3, the IC 50 values of bestatin for APN was calculated to be 5.90 ± 1.04 μM, which was in accordance with other studies reported previously (5.7 μM [ 19 ] and 4.57 μM [ 28 ] ).…”

Section: Resultssupporting

confidence: 90%

“…As shown in Figure 3, the IC 50 values of bestatin for APN was calculated to be 5.90 ± 1.04 μM, which was in accordance with other studies reported previously (5.7 μM [19] and 4.57 μM [28] ). Furthermore, we applied the developed CL method to establish a high-throughput screening system, which was used to evaluate the inhibitory activity of new compounds.…”

Section: Effects Of Inhibitors Against Apnsupporting

confidence: 90%

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A facile turn‐on chemiluminescence probe for sensitive imaging on aminopeptidase N activity

Sun

Mao

et al. 2022

Luminescence

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Aminopeptidase N, as a target for drug discovery, shows marked relationships with many diseases, especially liver injury and cancer. Here, we explored a chemiluminescence (CL) probe for sensing APN by tethering the APN‐specific substrate group to the ortho‐acrylated phenoxy‐dioxetane scaffold. In this way, two CL probes (APN‐CL and BAPN‐CL) were designed with noncapped leucine and butoxy‐carbonyl capped leucine as the protecting group to preserve the chemiexcitation energy. The uncovered leucine was demonstrated to be essential for detection of APN activity by comparing the CL intensity of two CL probes. Probe APN‐CL was turned on upon APN cleavage, resulting in a high chemiluminescent emission, whereas the chemiexcitation energy of probe BAPN‐CL was still restrained even with the high‐level APN. The result was further elucidated by molecular docking simulations. Probe APN‐CL exhibited a fast response and high sensitivity with a detection limit of 0.068 U/L, and an excellent specificity for the discrimination of APN from biological ions, small molecules, and other proteases commonly found in living system. By virtue of good stability and cell viability, probe APN‐CL imaged abnormal levels of APN in tumour cells and tumour‐bearing mice. Moreover, this probe APN‐CL could be easily used to evaluate APN inhibitors and APN levels in plasma samples from 20 patients. Overall, as a facile and cost‐effective probe, APN‐CL will be a promising alternative in the early diagnosis of pathologies and for cost‐effective screening of inhibitors.

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Section: Resultssupporting

confidence: 90%

Section: Effects Of Inhibitors Against Apnsupporting

confidence: 90%

A facile turn‐on chemiluminescence probe for sensitive imaging on aminopeptidase N activity

Sun

Mao

et al. 2022

Luminescence

View full text Add to dashboard Cite

show abstract

“…Abnormal expression or altered activity of LAPs can lead to tumor invasion 101 . In fact, overexpression of LAPs has been observed in various human diseases, including hepatocellular carcinoma, breast cancer, and endometrial cancer 99 , thereby indicating that LAPs are a potential tumor predictive marker 102 .…”

Section: Peptide/group Selective To Enzymesmentioning

confidence: 99%

Activated molecular probes for enzyme recognition and detection

Yuan¹,

Wu²,

Zhao³

et al. 2022

Theranostics

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Exploring and understanding the interaction of changes in the activities of various enzymes, such as proteases, phosphatases, and oxidoreductases with tumor invasion, proliferation, and metastasis is of great significance for early cancer diagnosis. To detect the activity of tumor-related enzymes, various molecular probes have been developed with different imaging methods, including optical imaging, photoacoustic imaging (PAI), magnetic resonance imaging, positron emission tomography, and so on. In this review, we first describe the biological functions of various enzymes and the selectively recognized chemical linkers or groups. Subsequently, we systematically summarize the design mechanism of imaging probes and different imaging methods. Finally, we explore the challenges and development prospects in the field of enzyme activity detection. This comprehensive review will provide more insight into the design and development of enzyme activated molecular probes.

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In vivo imaging via a red-emitting fluorescent probe to diagnosing liver cancer or drug-induced liver disease

Yang

Zhang

Li³

et al. 2021

Analytica Chimica Acta

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Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

Abstract: A specific reaction-based SERS approach was developed for the selective and sensitive detection of leucine aminopeptidase activity in serum.

Cited by 8 publications

References 47 publications

A facile turn‐on chemiluminescence probe for sensitive imaging on aminopeptidase N activity

A facile turn‐on chemiluminescence probe for sensitive imaging on aminopeptidase N activity

Activated molecular probes for enzyme recognition and detection

In vivo imaging via a red-emitting fluorescent probe to diagnosing liver cancer or drug-induced liver disease

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