Detection of Lewis antigen structural change by FTIR spectroscopy

Physicochemical Properties of African Catfish Mucus and Its Effect on the Stability of Soya Milk Emulsions

“…In the same way, studies by other authors revealed that amide I bands were found in the range 1600-1700 cm −1 with bands that show C-O stretching vibrations of protein-peptide bonds which is consistent with the results obtained in the ACM [28]. The distinctive absorption bands of 3301 cm −1 , 1634 cm −1 and 1539 cm −1 in the ACM designates the presence of peptide bonds [28,64,65,70].…”

“…The peak at 1414 cm −1 represents a C-O of carboxyl group accepting zero or one H-bond or O-H (alcohols) bend while the peak at 1136 cm −1 could represent anti-symmetric C-O-C glycoside bond, C-O strong bond stretch [29,68]. The peak at 1040 cm −1 is ascribed to a primary amine, stretching, secondary R-carbon of amino acids for glycoprotein or mucus while the peak at 867 cm −1 represents C-H out of plane bending [66,69,70]. In addition, the peaks at 626 cm −1 are attributed to C-H vibrating bends in RC=CH sites while peaks at 524 cm −1 are linked to S-S disulfide stretch in bio-polymers with proteins and collagen [69,71,72].…”

“…reported that although, Amide I, II, and III bands could be used to affirm protein secondary structure, FTIR investigations of amide I give better results as it is most responsive to changes in conformation and establish the presence of peptide bonds [27]. The peak of amide I occurs as a result of stretching vibration of C-O bonds and N-H bending while C-H stretching vibration is responsible for the Amide II band [27,70]. In the same way, studies by other authors revealed that amide I bands were found in the range 1600-1700 cm −1 with bands that show C-O stretching vibrations of protein-peptide bonds which is consistent with the results obtained in the ACM [28].…”

“…ACM also showed wavelength peaks of 1136 cm −1 , which suggested the possibility of anti-symmetric glycoside bond, C-O strong bond stretch [29,68,70,73] investigated the physicochemical characteristics of glycoprotein present in green algae and suggested that asymmetric stretch of bonds with intense bands around 1130 cm −1 could be used to identify glycosidic linkages. Furthermore, FTIR analysis of brown algae polysaccharides indicated peaks around 1135 cm −1 which are characteristics of vibrational stretches of the glycosidic C-O group of bio-polymers.…”

A Novel On‐Chip Method for Differential Extraction of Sperm in Forensic Cases

“…As the fingerprint region of SLeX was designated between 900 and 1280 cm −1 ,35 we first analyzed this range and observed C—H wagging at 962 cm −1 , C—O stretching/bending at 1025 and 1082 cm −1 , C—O stretching at 1134 cm −1 , and C—N stretching at 1181 cm −1 . These absorbance spectra values appear to be shifted from unbound SLeX molecule,35 which might be caused by the generation of chemical bonding during immobilization process. Since glass has intense characteristic spectrum between ≈682 cm −1 and ≈1200 cm −1 (e.g., Si—OH bending and Si—O—Si stretching),36, 37, 38 the signal intensities of peaks in this region reduced.…”