“…Its advantage over the traditional RT-PCR technique is its suitability for the direct amplification of RNA targets, obviating the need for thermal cycling equipment. NASBA has been successfully applied to the detection of pathogenic bacteria, such as Listeria monocytogenes (2,3), Campylobacter (26), and Mycobacterium (27). NASBA has also been used for the amplification of RNA from the human immunodeficiency virus in infected cells, blood, and plasma (12).…”