Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction

Border, Peter; Howard, Justin; Plastow, Graham; Siggens, K.

doi:10.1111/j.1472-765x.1990.tb00149.x

Cited by 185 publications

(116 citation statements)

References 15 publications

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“…detection of the hly gene, encoding the virulence factor listeriolysin O, and the highly conserved 23S rRNA genes of Listeria spp. (33). Subsequently, Listeria species were differentiated by multiplex PCR targeting the invasion-associated protein (iap) gene (34).…”

Section: Methodsmentioning

confidence: 99%

Reservoirs of Listeria Species in Three Environmental Ecosystems

Linke

Rückerl

Brugger

et al. 2014

Appl Environ Microbiol

165

182

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Soil and water are suggested to represent pivotal niches for the transmission of Listeria monocytogenes to plant material, animals, and the food chain. In the present study, 467 soil and 68 water samples were collected in 12 distinct geological and ecological sites in Austria from 2007 to 2009. Listeria was present in 30% and 26% of the investigated soil and water samples, respectively. Generally, the most dominant species in soil and water samples were Listeria seeligeri, L. innocua, and L. ivanovii. The human-and animal-pathogenic L. monocytogenes was isolated exclusively from 6% soil samples in regions A (mountainous region) and B (meadow). Distinct ecological preferences were observed for L. seeligeri and L. ivanovii, which were more often isolated from wildlife reserve region C (Lake Neusiedl) and from sites in proximity to wild and domestic ruminants (region A). The higher L. monocytogenes detection and antibiotic resistance rates in regions A and B could be explained by the proximity to agricultural land and urban environment. L. monocytogenes multilocus sequence typing corroborated this evidence since sequence type 37 (ST37), ST91, ST101, and ST517 were repeatedly isolated from regions A and B over several months. A higher L. monocytogenes detection and strain variability was observed during flooding of the river Schwarza (region A) and Danube (region B) in September 2007, indicating dispersion via watercourses.

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Section: Methodsmentioning

confidence: 99%

Reservoirs of Listeria Species in Three Environmental Ecosystems

Linke

Rückerl

Brugger

et al. 2014

Appl Environ Microbiol

165

182

View full text Add to dashboard Cite

show abstract

“…Select isolates were examined for Gram's reaction, catalase production, nitrate reduction, and motility on SIM medium, as well as for hemolysis and the CAMP reaction on 5% sheep blood agar (1). Isolates were further confirmed to be L. monocytogenes via PCR using primers targeting hlyA and genes 1 and 2 (2,24).…”

Section: Methodsmentioning

confidence: 99%

Retail Survey of Brazilian Milk and Minas Frescal Cheese and a Contaminated Dairy Plant To Establish Prevalence, Relatedness, and Sources of Listeria monocytogenes Isolates

Brito

Santos

Arcuri

et al. 2008

Appl Environ Microbiol

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A study was designed to recover Listeria monocytogenes from pasteurized milk and Minas frescal cheese (MFC) sampled at retail establishments (REs) and to identify the contamination source(s) of these products in the corresponding dairy processing plant. Fifty milk samples (9 brands) and 55 MFC samples (10 brands) were tested from REs located in Juiz de Fora, Minas Gerais, Brazil. All milk samples and 45 samples from 9 of 10 MFC brands tested negative for L. monocytogenes; however, "brand F" of MFC obtained from REs 119 and 159 tested positive. Thus, the farm/plant that produced brand F MFC was sampled; all samples from the milking parlor tested negative for L. monocytogenes, whereas several sites within the processing plant and the MFC samples tested positive. All 344 isolates recovered from retail MFC, plant F MFC, and plant F environmental samples were serotype 1/2a and displayed the same AscI or ApaI fingerprints. Since these results established that the storage coolers served as the contamination source of the MFC, plant F was closed so that corrective renovations could be made. Following renovation, samples from sites that previously tested positive for the pathogen were collected from the processing environment and from MFC on multiple visits; all tested negative for L. monocytogenes. In addition, on subsequent visits to REs 159 and 119, all MFC samples tested negative for the pathogen. Studies are ongoing to quantify the prevalence, levels, and types of L. monocytogenes in MFC and associated processing plants to lessen the likelihood of listeriosis in Brazil.

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“…10 Primers U1 (5Ј-CAG-GMG-CCG-CGG-TAA-TWC-3Ј), where M denotes A or C and W denotes A or T, and LI1 (5Ј-CTC-CAT-AAA-GGT-GAC-CCT-3Ј) target a 938-bp rRNA sequence in members of the genus Listeria. 5 Primers were commercially synthesized. c Amplification was performed in a 50-l volume containing 0.5 ng sample DNA; 50 pmole each of Lis-1, Lis-2, UI, and LI1; 1.25 U of Taq DNA polymerase d ; 200 M (each) dATP, dCTP, dTTP, and dGTP; 10 mM Tris-HCl; 50 mM KCl; and 6.5 mM MgCl 2 .…”

Section: Methodsmentioning

confidence: 99%

A Case Report of Sporadic Ovine Listerial Menigoencephalitis in Iowa with an Overview of Livestock and Human Cases

et al. 2002

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Abstract. A case of ovine listeriosis was examined in a flock of sheep. The index case was a male lamb, which was part of a flock of 85 sheep located in central Iowa. Because the sheep were raised on a premise where soybean sprouts were also cultivated for the organic foods market, the potential of a public health concern was addressed. To identify the source of contaminations, clinical and environmental samples were cultured for Listeria monocytogenes. Isolates were serotyped and analyzed using pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes (serotype 1) was recovered from the brain of a male lamb with clinical signs of listerial encephalitis. Isolates of serotypes 1 and 4 were also cultured from feces of clinically healthy lambs, compost piles, and soybean cleanings. By PFGE, the clinical isolate was distinctly different from the other isolates. Environmental isolates were identified as L. monocytogenes serotypes 1 and 4. However, by PFGE, none matched the profile of the single clinical isolate. Thus, the ultimate source of contamination is unknown.

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Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction

Cited by 185 publications

References 15 publications

Reservoirs of Listeria Species in Three Environmental Ecosystems

Reservoirs of Listeria Species in Three Environmental Ecosystems

Retail Survey of Brazilian Milk and Minas Frescal Cheese and a Contaminated Dairy Plant To Establish Prevalence, Relatedness, and Sources of Listeria monocytogenes Isolates

A Case Report of Sporadic Ovine Listerial Menigoencephalitis in Iowa with an Overview of Livestock and Human Cases

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