Detection of &lt;i&gt;Anaplasma phagocytophilum&lt;/i&gt; from Wild Boars and Deer in Japan

Masuzawa, Toshiyuki; Uchishima, Yoshiyuki; Fukui, Takashi; Okamoto, Yoshihiro; Muto, Maki; Koizumi, Nobuo; Yamada, Akio

doi:10.7883/yoken.64.333

Cited by 32 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An important consideration in research about Anaplasma phagocytophilum in wild animals is whether the detected strains may be potentially pathogenic to humans. Wild and game animals can be potential reservoirs of pathogenic A. phagocytophilum strains [24,42,43]. The present study used three genetic markers for analysis, viz.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genotyping of Anaplasma phagocytophilum Strains from Wild Animals, European Bison (Bison bonasus) and Eurasian Moose (Alces alces) in Poland

Myczka

Kaczor

Filip-Hutsch

et al. 2022

Animals

View full text Add to dashboard Cite

Wild large ungulates, like European bison (Bison bonasus) and Eurasian moose (Alces alces), form an important part of the circulation of Anaplasma phagocytophilum, a Gram-negative, intracellular, tick-transmitted bacterium, in the natural environment. Bison and moose tissue samples were subjected to 16S rDNA, groEL and ankA partial gene marker amplification with specific primers using various variants of PCR. Out of 42 examined individuals, Anaplasma sp. were detected in 4/13 Eurasian moose (31%) and 7/29 European bison (24%). In addition, 12 groEL and 5 ankA partial gene positive samples were obtained from the examined animals. The phylogenetic analysis of the groEL partial gene classified samples from European bison to ecotype I, and samples from Eurasian moose to ecotype I and II; the analysis of the ankA partial gene assigned the samples to clusters I and IV. This study extends knowledge about A. phagocytophilum in wild large ungulates in Poland. This is the first report about the occurrence of Anaplasma sp. in one of the largest populations of free living European bison in the world. Our findings confirm that strains of A. phagocytophilum from Bison bonasus and Alces alces may constitute a natural reservoir of pathogenic HGA Anaplasma strains.

show abstract

Section: Discussionmentioning

confidence: 99%