Detection of &lt;i&gt;van&lt;/i&gt;A-Containing &lt;i&gt;Enterococcus&lt;/i&gt; Species in Faecal Microbiota of Gilthead Seabream (&lt;i&gt;Sparus aurata&lt;/i&gt;)

Barros, Joana; Andrade, Margarida; Radhouani, Hajer; López, María José; Igrejas, Gilberto; Poeta, Patrícia; Torres, Carmén

doi:10.1264/jsme2.me11346

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…Interest in the detection of VRE has been increasing [36], given the global relevance of the problem. In Portugal, this resistance has been reported in several environments, with special incidence in wild animals from our research group [37][38][39][40].…”

Section: Discussionmentioning

confidence: 63%

Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

Sabença

Sousa

Oliveira

et al. 2020

Biology

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Vancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6’)-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 ± 3, and a peak at m/z 6048 ± 1 allowed us to distinguish Enterococcus faecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.

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Section: Discussionmentioning

confidence: 63%

Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

Sabença

Sousa

Oliveira

et al. 2020

Biology

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“…Enterococcus faecalis in this study were ascribed to STs ST6, ST16 and ST179. ST6 with the vanA gene was found in fish in Portugal (Barros et al ., ) but more often in human clinical isolates (Freitas et al ., ; Lopez et al ., ) and is related (DLV) to ST2, the founder of CC‐2. This clonal complex is associated with hospital environments and was defined as a high‐risk clonal complex (Ruiz‐Garbajosa et al ., ).…”

Section: Discussionmentioning

confidence: 99%

American crows as carriers of vancomycin‐resistant enterococci with vanA gene

Oravcová

Zurek

Townsend

et al. 2013

Environmental Microbiology

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We studied the vanA-carrying vancomycin-resistant enterococci (VRE) isolated from American crows in the United States during the winter 2011/2012. Faecal samples from crows were cultured selectively for VRE and characterized. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to examine epidemiological relationships of vanA-containing VRE. Isolates were tested in vitro for their ability to horizontally transfer the vancomycin resistance trait. VRE with the vanA gene were found in 15 (2.5%) of 590 crows samples, from which we obtained 22 different isolates. Enterococcal species were Enterococcus faecium (14) and E. faecalis (8). One, two and 19 isolates originated from Kansas, New York State and Massachusetts, respectively. Based on MLST analysis, E. faecium isolates were grouped as ST18 (6 isolates), ST555 (2), and novel types ST749 (1), ST750 (3), ST751 (1), ST752 (1). Enterococcus faecalis isolates belonged to ST6 (1), ST16 (3) and ST179 (4). All isolates were able to transfer the vancomycin resistance trait via filter mating with very high transfer range. Clinically important enterococci with the vanA gene occur in faeces of wild American crows throughout the United States. These migrating birds may contribute to the dissemination of VRE in environment over large distances. [Correction added after first online publication on 06 August 2013: The number of E. faecium ST752 isolate is now amended to '1', consistent with that shown in the 'Results' section and Figure 2.].

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“…strains resistant to vancomycin in S. aurata from aquaculture. This resistance was already found in this environment but in Enterococcus species [ 87 ]. Acquired reduced susceptibility to mupirocin in S. petrasii could be related to mutations in the ileS gene and, more rarely, to the acquisition of plasmid-mediated mupA gene, like in S. aureus [ 116 ].…”

Section: Discussionmentioning

confidence: 52%

“…Interestingly, although banned for use in food-producing animals in many countries (since 1994 in Europe; [ 98 ]), decreased chloramphenicol susceptibility continues to be described, not only in the present study (with MIC of 32 to > 64 mg/l), but also in others, with the presence of cat genes [ 87 , 99 ]. Chloramphenicol can occur naturally, produced by soil organisms such as Streptomyces venezuelae .…”

Section: Discussionmentioning

confidence: 54%

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Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

et al. 2020

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In a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of “critically important antimicrobials” and “highly important antimicrobials” for human medicine.

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Detection of <i>van</i>A-Containing <i>Enterococcus</i> Species in Faecal Microbiota of Gilthead Seabream (<i>Sparus aurata</i>)

Cited by 19 publications

References 24 publications

Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

American crows as carriers of vancomycin‐resistant enterococci with vanA gene

Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

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