Detection of lysozyme adsorption on titania using surface plasmon resonance on Au layer coated with thin titania film

Abstract

Titanium metal, which is used as artificial bone, has a titania surface layer. In this study, titania was investigated using surface plasmon resonance (SPR) in terms of protein adsorption, which occurs during the initial process of cellular attachment by employing a multilayer device consisting of a titania layer on Au. The titania film was deposited on the Au layer in the multilayer device by atmospheric chemical vapor deposition. A flow cell was used to evaluate protein adsorption on the titania top layer of the multilayer device. Phosphor buffer solutions with and without the protein lysozyme (Lyz) were injected into the flow cell. From the attenuation of reflected light, the SPR angle was determined as the angle with minimum reflection intensity. The observed behavior of the SPR angle indicated that Lyz was adsorbed on the titania film surface, and the obtained SPR angles increased with the Lyz concentration on the titania surface. These results indicate that the titania/Au multilayer SPR device can easily be used to detect the adsorption of protein.

Heterolayered Films of Monolayer WS2 Nanosheets on Monolayer Graphene Embedded in Poly(methyl methacrylate) for Plasmonic Biosensing

“…The usage of MoS 2 nanosheets provides a direct and facile method for immobilizing protein molecules on the sensor surface with the help of hydrophobic interactions. Most of the few earlier works that have used TMDC materials for plasmonic sensing have utilized nonprism-based customized configurations like optical fiber with the wavelength interrogation technique. − 2D materials like MoS 2 , antimonene, and graphene have been used for the detection of bovine serum albumin (BSA), micro-ribonucleic acid (miRNA), para-aminobenzoic acid (PABA), and Escherichia coli (E. coli). ,− Kaushik et al designed a fiber-optic-based SPR sensor for detecting BSA. The detection limit was 0.29 μg/mL.…”

Amphiphilic diblock copolymer‐based multiagent photonic sensing scheme

“…Due to its wide use as a food preservative, there has been an extensive study on lysozyme's physical and chemical properties, and in context, the electrostatic characteristics of lysozyme has been extensively investigated . A number of different and quite diverse approaches for lysozyme detection have been developed, such as aptasensors where based on electrochemical interactions, a high degree of selectivity in the coexistence of other proteins has been achieved, electrochemical impedance spectroscopy based on antilysozyme aptamer as molecular recognition element, monitoring of atomic force microscopy cantilevers' deflection and bending under electrostatic interactions, bulk acoustic wave viscosity sensor, and surface plasmon resonance detection schemes have been also widely utilized for lysozyme sensing …”

Novel Approach for Lysozyme Detection Employing Block Copolymer Overlayers on Plastic Optical Fibers

“…Moreover, a method for determination of lysozyme with a Bulk Acoustic Wave (BAW) viscosity sensor has been presented [9], based on the bacteriolytic action of lysozyme and the response of the sensor to the viscosity and density change of this process. Surface plasmon resonance (SPR) phenomenon has been also widely utilized for lysozyme sensing [10,11]. Although all the aforementioned techniques achieve a remarkable degree of sensitivity, the use of expensive and complex setups which follow time consuming processes makes them inapplicable in many cases.…”