Detection of Malaria Parasites After Treatment in Travelers: A 12-months Longitudinal Study and Statistical Modelling Analysis

Homann, Manijeh Vafa; Emami, S. Noushin; Yman, Victor; Stenström, Christine; Sondén, Klara; Ramström, Hanna; Karlsson, Mattias; Asghar, Muhammad; Färnert, Anna

doi:10.1016/j.ebiom.2017.10.003

Cited by 56 publications

(39 citation statements)

References 30 publications

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“…This result needs nevertheless to be considered with caution. PCR methods are known to be very sensitive and may, in certain cases, detect DNA of parasites in the blood of a wrong host after the parasites have died (Vafa Homann et al, ). The only valid proof of an infection would be the observation of the parasites in a blood smear or, at least, if only molecular analyses are available, confirmation of infection in multiple individuals of the same species.…”

Section: Discussionmentioning

confidence: 99%

Extensive diversity of malaria parasites circulating in Central African bats and monkeys

Boundenga

Ngoubangoye

Mombo

et al. 2018

Ecology and Evolution

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The order Haemosporidia gathers many protozoan parasites which are known to infect many host species and groups. Until recently, the studies on haemosporidian parasites primarily focused on the genus Plasmodium among a wide range of hosts. Genera, like the genus Hepatocystis, have received far less attention. In the present study, we present results of a survey of the diversity of Hepatocystis infecting bats and monkeys living in a same area in Gabon (Central Africa). Phylogenetic analyses revealed a large diversity of Hepatocystis lineages circulating among bats and monkeys, among which certain were previously observed in other African areas. Both groups of hosts harbor parasites belonging to distinct genetic clades and no transfers of parasites were observed between bats and monkeys. Finally, within each host group, no host specificity or geographical clustering was observed for the bat or the primate Hepatocystis lineages.

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Section: Discussionmentioning

confidence: 99%

Extensive diversity of malaria parasites circulating in Central African bats and monkeys

Boundenga

Ngoubangoye

Mombo

et al. 2018

Ecology and Evolution

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“…Persistence of parasite DNA after successful therapy has been widely documented but was systematically investigated in malaria patients only recently. Homann and colleagues showed, using blood from 31 successfully treated malaria patients, that DNA could be detected by msp2-and species-specific PCR assays up to 31 and 42 days after treatment, respectively, whereas parasites could be detected by microscopy up to 2 days after treatment (30).…”

Section: Discussionmentioning

confidence: 99%

BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection

Mootien¹,

Lawres

Perrin

et al. 2018

J Clin Microbiol

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Human babesiosis is an emerging zoonotic infectious disease caused by intraerythrocytic protozoan parasites of the genus Most cases of human babesiosis are caused by and often manifest in individuals over the age of 50 years or in patients with a compromised immune system. Patients who develop symptomatic infections usually experience months of asymptomatic infection after the acute infection has resolved. About one-fifth of-infected adults never develop symptoms. These asymptomatically infected individuals sometimes donate blood and thus can transmit through blood transfusion. Current assays for detection of active infections can be used to screen donor blood prior to transfusion, but they rely primarily on microscopy or PCR methods, which have sensitivity and technical limitations. Here we report the development of an antigen capture enzyme-linked immunosorbent assay (BmGPAC) based on a major secreted immunodominant antigen of (BmGPI12/BmSA1), and we provide evidence that this assay is superior for detection of active infections, compared to available microscopy methods and serological assays. The assay has been evaluated using supernatants of -infected erythrocytes cultured, sera from -infected laboratory mice, and sera from wild mice and human patients. Our data suggest that the BmGPAC assay is a reliable assay for detection of active infections and is superior to real-time PCR and antibody assays for diagnosis of acute infections, screening of the blood supply, and epidemiological surveys of humans and animal reservoir hosts.

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“…Dried blood spots on filter papers obtained at each monthly visit and at delivery were punched, and three circles of 3 mm in diameter were used for DNA extraction using QIAamp DNA blood micro kit (Qiagen GmbH, Hilden, Germany) following the manufacturer's recommendations. Real-time PCR was used to qualitatively detect Plasmodium infection (P. falciparum, P. vivax, P. ovale, and P. malariae) in the 7500 Fast Real-Time PCR system (Applied Biosystems), following a previously described protocol [46]. The master mix for a single reaction included species-specific probes and forward primers for all four Plasmodium species used in combination with a conserved reverse primer.…”

Section: Detection Of Malaria By Real-time Polymerase Chain Reaction mentioning

confidence: 99%

Effectiveness of Sulfadoxine–Pyrimethamine for Intermittent Preventive Treatment of Malaria and Adverse Birth Outcomes in Pregnant Women

et al. 2020

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Effectiveness of intermittent preventive treatment in pregnancy with sulfadoxine–pyrimethamine (IPTp-SP) for prevention of malaria and adverse birth outcomes can be compromised by parasites-resistance to sulfadoxine–pyrimethamine. This study prospectively evaluated the effectiveness of IPTp-SP in Southeast Tanzania. From January 2017 to May 2019, HIV-negative and malaria-negative (mRDT) pregnant women attending their first antenatal-care visit in the second or third trimester (n = 500) were enrolled to receive monthly IPTp-SP and followed the protocol till delivery. The primary outcome was the prevalence of histopathological placental malaria. Secondary outcomes were anemia, malaria parasites detected during pregnancy and at delivery, adverse birth outcomes (low-birth-weight [LBW], premature birth, fetal anemia, still birth, and spontaneous abortion). Rates of histopathological placental malaria, any parasitemia at delivery (placental, cord or maternal), and any adverse birth outcome were 9.4%, 20.9%, and 26.5%, respectively. Rates of symptomatic malaria and parasitemia during pregnancy were 2.8% and 16%, respectively. Histopathological placental malaria significantly increased the odds of any adverse birth outcomes, particularly LBW. IPTp-SP with more than or equal to three doses significantly improved birth weight and reduced the risk of LBW by 56% compared to <3 SP doses (p = 0.009). IPTp-SP with more than or equal to three doses is still effective in improving birth weight. However, the detection of histopathological placental-malaria in one-tenth and parasitemia in one-fifth of pregnant women reflects the need to optimize the prevention of malaria during pregnancy.

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Detection of Malaria Parasites After Treatment in Travelers: A 12-months Longitudinal Study and Statistical Modelling Analysis

Cited by 56 publications

References 30 publications

Extensive diversity of malaria parasites circulating in Central African bats and monkeys

Extensive diversity of malaria parasites circulating in Central African bats and monkeys

BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection

Effectiveness of Sulfadoxine–Pyrimethamine for Intermittent Preventive Treatment of Malaria and Adverse Birth Outcomes in Pregnant Women

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