Detection of mammagloblin by RT-PCR as a biomarker for lymph node metastasis in breast cancer patients: A systematic review and meta-analysis

Monsalve-Lancheros, Ana Milena; Ibáñez-Pinilla, Milcíades; Ramírez-Clavijo, Sandra

doi:10.1371/journal.pone.0216989

Cited by 10 publications

(12 citation statements)

References 33 publications

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“…It is noteworthy that RT-PCR found broad applications in diagnostics and treatment monitoring in oncology [ 53 ]. It has been used in research of prognostic and predictive tumor biomarkers, e.g., in breast cancer [ 54 ], melanoma [ 55 , 56 ], or hepatocellular carcinoma [ 57 , 58 ], as well as for other purposes, such as micrometastasis determination [ 59 ] or detection of circulating tumor cells [ 60 , 61 ]. In this method complementary DNA (cDNA) is generated from RNA template in reverse transcription and specific DNA targets are amplified using polymerase chain reaction (PCR), employing large set of primer pairs.…”

Section: Transcriptome’s Components and Methods Of Its Analysismentioning

confidence: 99%

Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine

Supplitt

Karpiński

Sasiadek

et al. 2021

IJMS

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Over the last decades, transcriptome profiling emerged as one of the most powerful approaches in oncology, providing prognostic and predictive utility for cancer management. The development of novel technologies, such as revolutionary next-generation sequencing, enables the identification of cancer biomarkers, gene signatures, and their aberrant expression affecting oncogenesis, as well as the discovery of molecular targets for anticancer therapies. Transcriptomics contribute to a change in the holistic understanding of cancer, from histopathological and organic to molecular classifications, opening a more personalized perspective for tumor diagnostics and therapy. The further advancement on transcriptome profiling may allow standardization and cost reduction of its analysis, which will be the next step for transcriptomics to become a canon of contemporary cancer medicine.

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Section: Transcriptome’s Components and Methods Of Its Analysismentioning

confidence: 99%

Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine

Supplitt

Karpiński

Sasiadek

et al. 2021

IJMS

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show abstract

“…The Q-RT-PCR technique is a rapid, cost-effective, and high-specificity assay for the assessment of gene expression [ 12 ]. Q-RT-PCR technique has significant potential in biomarker detection relating to mammaglobin (MGB) and metastasis of the lymph nodes [ 17 ]. For example, it has been recently shown that RT-PCR had a considerable diagnostic prediction for detecting mammaglobin biomarkers for lymph node metastasis in breast cancer patients [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Association study of Bif-1 gene expression with histopathological characteristics and hormone receptors in breast cancer

Mohammadi¹,

Salimi

Angaji

et al. 2022

BMC Women's Health

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Background Breast cancer is a heterogeneous disease that has various clinical outcomes. Bax-interacting factor-1 (Bif-1) is a member of the endophilin B family that generates the pro-apoptotic BCL2-Associated X (BAX) protein in response to apoptotic signals. Lack of Bif-1 inhibits the intrinsic pathway of apoptosis and enhancements the risk of tumor genesis. The present study aimed to investigate the relationship between hormone receptors (ER, PR, and HER2) status and different levels of Bif-1 gene expression in breast cancer patients. Methods Bif-1 gene expression was evaluated in 50 breast cancer tumors and 50 normal breast mammary tissues using the SYBR Green real-time RT-PCR technique. Multivariate and univariate analyses were used to appraise the relationship between the prognostic significance of the Bif-1 gene using SPSS software. In this study, the Bif-1 was selected as a candidate for a molecular biomarker and its expression status in breast cancer patients with hormone receptors (ER, RR, and HER2) compared to patients without these hormone receptors. Results The study showed that the relative expression of the Bif-1 gene in tissues of patients with hormone receptors in breast cancer compared to those without hormone receptors was not statistically significant. The expression levels of the Bif-1 gene in different groups were evaluated for hormone receptor status. No significant relationship was found between the Bif-1 gene expression and hormone receptors (ER, PR, and HER2) (p > 0.05). Conclusion Bif-1 gene expression may be a useful prognostic marker in breast cancer.

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“…A meta-study systematically evaluated the clinical efficacy and cost-effectiveness of one-step nucleic acid amplification (OSNA) and Metasin in the intraoperative analysis of SLN metastasis in breast cancer patients. And proposed that the OSNA test only analyzes cytokeratin 19 (CK19) gene expression, while the Metasin analysis analyzes CK19 and mammaglobin gene expression ( 6 ). In this study, we focused more on intraoperative analysis of the plausibility of the expression of two markers in sentinel lymph nodes as biomarkers for the presence of metastases.…”

Section: Introductionmentioning

confidence: 99%

A clinical diagnostic test on the detection of sentinel lymph node metastasis in breast neoplasms using a 1-step RT-PCR

et al. 2022

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Background: Currently, assessment of sentinel lymph node (SLN) requires cytology, hematoxylin-eosin (HE), and immunohistochemistry (IHC). However, routine pathological slides still suffer from certain sampling errors and have time limitations. This study sought to investigate the sensitivity and specificity of SLN detection by reverse transcription-polymerase chain reaction (RT-PCR), which quantifies the expression of mammaglobin and cytokeratin-19 genes to determine SLN status. Methods: The RT-PCR detection of cycles threshold (CT) values has a direct relationship with the lymph node metastasis. This study prospectively collected 256 sentinel lymph nodes from 150 patients diagnosed with breast cancer between August and November 2017. In the detection of metastases in lymph nodes, molecular markers can be verified at the cell-level and tissue-level of tumor cells. In this study, IHC results were used as the gold standard for judging sentinel lymph node status. Results: (I) According to the established cell models, as the lymph nodes in tumor cells increase, RT-PCR CT values decrease. (II) 83 lymph nodes were first collected, and the interpretation criteria for the molecular detection results were determined based on the immunohistochemistry (IHC) results. (III) The statisticalanalysis showed that the sensitivity of the RT-PCR was 80.49% and the specificity was 91.55%. The positive predictive and negative predictive values were 64.71% and 96.06%, respectively. There was no significant difference between RT-PCR detection and IHC detection (P=0.076). Statistical chi-square analysis also showed that the difference between intraoperative freezing and immunohistochemistry was statistically significant (P=0.000). There was a statistically significant difference between intraoperative freezing and RT-PCR detection (P=0.000). RT-PCR detection is more sensitive than intraoperative frozen detection, and is closer to the results of immunohistochemistry.Conclusions: RT-PCR had objective and rapid output advantages, and was proven to be true and reliable.RT-PCR detection can not only rapidly assess sentinel lymph node status in breast cancer patients during surgery, but its accuracy is also close to that of IHC. Correctly determine whether to perform axillary lymph node dissection and improve the survival rate of patients.

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Detection of mammagloblin by RT-PCR as a biomarker for lymph node metastasis in breast cancer patients: A systematic review and meta-analysis

Cited by 10 publications

References 33 publications

Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine

Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine

Association study of Bif-1 gene expression with histopathological characteristics and hormone receptors in breast cancer

A clinical diagnostic test on the detection of sentinel lymph node metastasis in breast neoplasms using a 1-step RT-PCR

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