Detection of marine neurotoxins in food safety testing using a multielectrode array

Nicolas, Jonathan; Hendriksen, Peter J.M.; Kleef, Regina G.D.M. van; Groot, Aart de; Bovee, Toine F.H.; Rietjens, Ivonne M.C.M.; Westerink, Remco H.S.

doi:10.1002/mnfr.201400479

Cited by 69 publications

(77 citation statements)

References 34 publications

(45 reference statements)

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“…Since K + channel blockers tested negative their integrity was confirmed by showing their activity towards other endpoints. Amiodarone inhibited neuronal activity in rat cortical neurons [37]. Measuring effects on the beating rate or on the generation of action potentials as additional parameters, using for example multielectrode arrays [38], will most likely result in a more sensitive assay and might even result in an assay able to detect a wider range of neurotoxins.…”

Section: Discussionmentioning

confidence: 99%

In vitro detection of cardiotoxins or neurotoxins affecting ion channels or pumps using beating cardiomyocytes as alternative for animal testing

Nicolas

Hendriksen

Haan

et al. 2015

Toxicology in Vitro

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In vitro detection of cardiotoxins or neurotoxins affecting ion channels or pumps using beating cardiomyocytes as alternative for animal testing Nicolaas, J.; Hendriksen, P.J.M.; Haan, L.H.J. de; Koning, R.; Rietjens, I.M.C.M.; Bovee, T.F.H.This is a "Post-Print" accepted manuscript, which has been published in the "Toxicology in Vitro"This version is distributed under a non-commencial no derivatives Creative Commons (CC-BY-NC-ND) user license, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and not used for commercial purposes. Further, the restriction applies that if you remix, transform, or build upon the material, you may not distribute the modified material.Please cite this publication as follows:

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Section: Discussionmentioning

confidence: 99%

In vitro detection of cardiotoxins or neurotoxins affecting ion channels or pumps using beating cardiomyocytes as alternative for animal testing

Nicolas

Hendriksen

Haan

et al. 2015

Toxicology in Vitro

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“…As such, MEA recordings allow for reproducible screening of neurotoxic compounds with high sensitivity and specificity (McConnell et al, 2012;Valdivia et al, 2014;Nicolas et al, 2014). Despite the marked increase in mitochondrial activity, MEA recordings demonstrated that neuronal activity of primary cortical cultures is largely unaffected upon acute (30 min) exposure to TCP isomers, mixtures and CBDP (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…First, effects on cell viability and mitochondrial activity were assessed. Next, effects of TCPs on spontaneous neuronal electrical activity were assessed using microelectrode array (MEA) recordings as a noninvasive in vitro neurotoxicity screening method (Hogberg et al, 2011;McConnell et al, 2012;Valdivia et al, 2014;Nicolas et al, 2014). Lastly, effects on neurite outgrowth were measured as a specific, integrated measure of neurotoxicity that represents the effects of multiple underlying targets (Harrill et al, 2011).…”

Section: Tablementioning

confidence: 99%

“…Primary cultures of rat cortical neurons were prepared from postnatal day 0 to 1 Wistar rat pups as described previously (Nicolas et al, 2014;Meijer et al, 2015;de Groot et al, 2016), with minor modifications. Briefly, pups were decapitated and cortices were rapidly dissected on ice and minced into small pieces, which were mechanically dissociated by gentle trituration and filtered through a cell strainer (BD Falcon, 100 mm nylon).…”

Section: Cell Culturementioning

confidence: 99%

“…Spontaneous electrical activity was recorded at DIV9-11 at a constant temperature of 37 C using a Maestro 768-channel amplifier with integrated heating system and temperature controller (Axion Biosystems Inc.) as described previously (Nicolas et al, 2014;de Groot et al, 2014). Axion's Integrated Studio (AxIS 1.7.8) was used to manage data acquisition.…”

Section: Measurements Of Neuronal Electrical Activity Using Microelecmentioning

confidence: 99%

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In vitro neurotoxic hazard characterization of different tricresyl phosphate (TCP) isomers and mixtures

et al. 2017

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A B S T R A C TExposure to tricresyl phosphates (TCPs), via for example contaminated cabin air, has been associated with health effects including the so-called aerotoxic syndrome. While TCP neurotoxicity is mainly attributed to ortho-isomers like tri-ortho-cresyl phosphate (ToCP), recent exposure and risk assessments indicate that ToCP levels in cabin air are very low. However, the neurotoxic potential of non-ortho TCP isomers and TCP mixtures is largely unknown. We therefore measured effects of exposure (up to 48 h) to different TCP isomers, mixtures and the metabolite of ToCP (CBDP: cresyl saligenin phosphate) on cell viability and mitochondrial activity, spontaneous neuronal electrical activity, and neurite outgrowth in primary rat cortical neurons.The results demonstrate that exposure to TCPs (24-48 h, up to 10 mM) increases mitochondrial activity, without affecting cell viability. Effects of acute TCP exposure (30 min) on neuronal electrical activity are limited. However, electrical activity is markedly decreased for the majority of TCPs (10 mM) following 48 h exposure. Additional preliminary data indicate that exposure to TCPs (48 h, 10 mM) did not affect the number of neurites per cell or average neurite length, except for TmCP and the analytical TCP mixture (Sigma) that induced a reduction of average neurite length.The combined neurotoxicity data demonstrate that the different TCPs, including ToCP, are roughly equipotent and a clear structure-activity relation is not apparent for the studied endpoints. The noobserved-effect-concentrations (1 mM) are well above current exposure levels indicating limited neurotoxic health risk, although exposures may have been higher in the past. Moreover, prolonged and/or repeated exposure to TCPs may exacerbate the observed neurotoxic effects, which argues for additional research.

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The Determination of Marine Biotoxins in Seafood

Gerssen

Klijnstra

2017

Analysis of Food Toxins and Toxicants

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Detection of marine neurotoxins in food safety testing using a multielectrode array

Cited by 69 publications

References 34 publications

In vitro detection of cardiotoxins or neurotoxins affecting ion channels or pumps using beating cardiomyocytes as alternative for animal testing

In vitro detection of cardiotoxins or neurotoxins affecting ion channels or pumps using beating cardiomyocytes as alternative for animal testing

In vitro neurotoxic hazard characterization of different tricresyl phosphate (TCP) isomers and mixtures

The Determination of Marine Biotoxins in Seafood

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