Abstract:Melanoma is responsible for more than half of the deaths related to skin cancer in the last few decades. A dual‐modality optical biopsy system with Raman spectroscopy and optical coherence tomography approach was built with the goal of achieving noninvasive skin measurement. To mimic melanoma and evaluate the effect of melanin on skin, models have been created by dissolving synthetic melanin in dimethyl sulfoxide and adding it to fresh skin samples. Compared to the untreated samples, morphological images showe… Show more
“…Moving the UST back enables optical access to the opening of WT from above and facilitates its combination with other optical modalities, such as OCT and RS. These modalities are currently being studied in other publications 41 . Figure 1 Sketch of the experimental setup.…”
Section: Methodsmentioning
confidence: 99%
“…The acoustical mirror is translatable within the tank, allowing for either precise positioning of the measurement plane or for C-mode imaging by using sequential steps with the attached motorized stage. Since the setup has an unobstructed optical view from the top, it can be easily integrated with other optical modalities, where Raman spectroscopy (RS) for melanoma dignity has the greatest potential 39 – 41 . The axial and lateral resolutions of each modality are measured on several self-designed phantoms and the setup is validated with preliminary measurements of in vivo imaging on human skin that are compared with histological measurement.…”
Accurate assessment of the size and depth of infiltration is critical for effectively treating and removing skin cancer, especially melanoma. However, existing methods such as skin biopsy and histologic examination are invasive, time-consuming, and may not provide accurate depth results. We present a novel system for simultaneous and co-localized ultrasound and photoacoustic imaging, with the application for non-invasive skin lesion size and depth measurement. The developed system integrates an acoustical mirror that is placed on an ultrasound transducer, which can be translated within a flexible water tank. This allows for 3D (C-mode) imaging, which is useful for mapping the skin structure and determine the invasion size and depth of lesions including skin cancer. For efficient reconstruction of photoacoustic images, we applied the open-source MUST library. The acquisition time per 2D image is <1 s and the pulse energies are below the legal Maximum Permissible Exposure (MPE) on human skin. We present the depth and resolution capabilities of the setup on several self-designed agar phantoms and demonstrate in vivo imaging on human skin. The setup also features an unobstructed optical window from the top, allowing for simple integration with other optical modalities. The perspective towards clinical application is demonstrated.
“…Moving the UST back enables optical access to the opening of WT from above and facilitates its combination with other optical modalities, such as OCT and RS. These modalities are currently being studied in other publications 41 . Figure 1 Sketch of the experimental setup.…”
Section: Methodsmentioning
confidence: 99%
“…The acoustical mirror is translatable within the tank, allowing for either precise positioning of the measurement plane or for C-mode imaging by using sequential steps with the attached motorized stage. Since the setup has an unobstructed optical view from the top, it can be easily integrated with other optical modalities, where Raman spectroscopy (RS) for melanoma dignity has the greatest potential 39 – 41 . The axial and lateral resolutions of each modality are measured on several self-designed phantoms and the setup is validated with preliminary measurements of in vivo imaging on human skin that are compared with histological measurement.…”
Accurate assessment of the size and depth of infiltration is critical for effectively treating and removing skin cancer, especially melanoma. However, existing methods such as skin biopsy and histologic examination are invasive, time-consuming, and may not provide accurate depth results. We present a novel system for simultaneous and co-localized ultrasound and photoacoustic imaging, with the application for non-invasive skin lesion size and depth measurement. The developed system integrates an acoustical mirror that is placed on an ultrasound transducer, which can be translated within a flexible water tank. This allows for 3D (C-mode) imaging, which is useful for mapping the skin structure and determine the invasion size and depth of lesions including skin cancer. For efficient reconstruction of photoacoustic images, we applied the open-source MUST library. The acquisition time per 2D image is <1 s and the pulse energies are below the legal Maximum Permissible Exposure (MPE) on human skin. We present the depth and resolution capabilities of the setup on several self-designed agar phantoms and demonstrate in vivo imaging on human skin. The setup also features an unobstructed optical window from the top, allowing for simple integration with other optical modalities. The perspective towards clinical application is demonstrated.
Cutaneous melanoma is the most lethal skin cancer and noninvasively distinguishing it from benign tumor is a major challenge. Raman spectroscopic measurements were conducted on 65 suspected melanocytic lesions and surrounding healthy skin from 47 patients. Compared to the spectra of healthy skin, spectra of melanocytic lesions exhibited lower intensities in carotenoid bands and higher intensities in lipid and melanin bands, suggesting similar variations in the content of these components. Distinct variations were observed among the autofluorescence intensities of healthy skin, benign nevi and malignant melanoma. By incorporating autofluorescence information, the classification accuracy of the support vector machine for spectra of healthy skin, nevi, and melanoma reached 90.2%, surpassing the 87.9% accuracy achieved without autofluorescence, with this difference being statistically significant. These findings indicate the diagnostic value of autofluorescence intensity, which reflect differences in fluorophore content, chemical composition, and structure among healthy skin, nevi, and melanoma.
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