Detection of Melanoma-Associated Antigen p97 in Human Malignant Melanoma Cell Lines by Immunoelectron Microscopy

The expression of a melanoma-associated antigen (MAA), p97, on the cell surfaces of five human .melanoma cell lines was investigated with the recently developed mixed passive hemagglutination (MPHA) method. Two of these lines, HMV-I and HMV-II, were subclones separated by cloning from a common original cell line. In all cell lines except HMV-I, definite expression of p97 antigen was detected in MPHA. However, virtually no expression ofthe antigen was observed in the HMV-I cell line. These results were confirmed by immunoelectron microscopic study. The present study is meaningful for the following two reasons. First, it was shown that MPHA is a sensitive and conventional method for detection of tumor-associated surface antigens on cultured neoplastic cells. Second, this is the first report ofclonal variation in expression of p97 antigen between subclones of a human melanoma cell line. Clonal variation in expression of a MAA as shown in the present study may cause serious problems when a monoclonal antibody is used for immunodiagnosis or immunotherapy.

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“…The procedures for immunoelectron microscopy for detection of p97 antigen have been described elsewhere (12). Briefly, melanoma cells were har- …”

Section: Immunoeledron Microscopymentioning

confidence: 99%

Clonal Variation in Expression of p97 Antigen in Human Melanoma Cell Lines Detected by Mixed Passive Hemagglutination

Saida

Uno

1987

The Journal of Dermatology

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Detection of Melanoma-Associated Antigen p97 in Human Malignant Melanoma Cell Lines by Immunoelectron Microscopy

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Clonal Variation in Expression of p97 Antigen in Human Melanoma Cell Lines Detected by Mixed Passive Hemagglutination

Clonal Variation in Expression of p97 Antigen in Human Melanoma Cell Lines Detected by Mixed Passive Hemagglutination

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