Detection of Merkel cell polyomavirus and human papillomavirus DNA in porocarcinoma

Urso, Carmelo; Pierucci, Federica; Sollai, Mauro; Arvia, Rosaria; Massi, Daniela; Zakrzewska, K.

doi:10.1016/j.jcv.2016.03.008

Cited by 16 publications

(28 citation statements)

References 22 publications

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“…97 Viral load monitoring is the recommended screening method to validate the failure of antiretroviral therapy. 98 Recently, the development of ddPCR has created an enormous opportunity for progress in HIV research, diagnosis, and care, including the following: quantification of proviral HIV-1 DNA 66,[99][100][101][102][103] and RNA, 28,71,101 optimize monitoring of ART-treated patients 104 HSCs stem-cell transplantation, 91,105 monitoring of ART concentrations, 106 detection of clinically relevant rare viral variants, test novel antiviral drugs, single-cell assays of HIV-infected cell lines, 107 applicable in HIV models, [108][109] vaccine 110 and dynamic of HIV provirus. 73,111 The advances in the field of ddPCR provide the opportunity to analyze viral proviral DNA (DNA-based viral load test) and CA viral RNA (RNA-based viral load test) for the enumeration and monitoring of host immune response to HIV.…”

Section: Human Immunodeficiency Virusmentioning

confidence: 99%

“…False-positive results are the major disadvantages of digital PCR to accurately measure the proviral DNA/RNA reservoir. 99 There are also a variety of technical limitations, which should be considered when using ddPCR. The software's automated threshold is usually too low and falsely considered a positive droplet, regardless of whether it has low fluorescence.…”

Section: Human Immunodeficiency Virusmentioning

confidence: 99%

“…False‐positive results are the major disadvantages of digital PCR to accurately measure the proviral DNA/RNA reservoir 99 . There are also a variety of technical limitations, which should be considered when using ddPCR.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

163

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High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated stochastically inside the microdroplets as reaction chambers. A small percentage of the reaction chamber contains one or fewer copies of the DNA or RNA. After PCR amplification, concentrations are determined based on the proportion of nonfluorescent partitions through the Poisson distribution. Some of the main features of ddPCR include high sensitivity and specificity, absolute quantification without a standard curve, high reproducibility, good tolerance to PCR inhibitor, and high efficacy compared to conventional molecular methods. These advantages make ddPCR a valuable addition to the virologist's toolbox. The following review outlines the recent technological advances in ddPCR methods and their applications in viral identification.

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Section: Human Immunodeficiency Virusmentioning

confidence: 99%

Section: Human Immunodeficiency Virusmentioning

confidence: 99%

See 1 more Smart Citation

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

163

View full text Add to dashboard Cite

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“…The clear cell variant of porocarcinoma is rare. [ 2 ] We describe the presence of clear cell porocarcinoma in one patient with EV.…”

mentioning

confidence: 99%

“…demonstrated the presence of β-HPV in porocarcinomas, and HPV14d was found in our patient's tumor, we hypothesized this may not be a coincidental finding. [ 2 ] We propose that EV-associated defects in cell-mediated immunity, together with immunosuppressive cytokines and p53 abnormalities, predisposed the patient to developing clear cell porocarcinoma.…”

mentioning

confidence: 99%

Clear Cell Porocarcinoma Arising in an Epidermodysplasia Verruciformis Patient

Souza

Valente

Tyring

et al. 2021

Indian Journal of Dermatology

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Merkel Cell Polyomavirus: A New DNA Virus Associated with Human Cancer

MacDonald

You

2017

Advances in Experimental Medicine and Biology

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Merkel cell polyomavirus (MCPyV or MCV) is a novel human polyomavirus that has been discovered in Merkel cell carcinoma (MCC), a highly aggressive skin cancer. MCPyV infection is widespread in the general population. MCPyV-associated MCC is one of the most aggressive skin cancers, killing more patients than other well-known cancers such as cutaneous T-cell lymphoma and chronic myelogenous leukemia (CML). Currently, however, there is no effective drug for curing this cancer. The incidence of MCC has tripled over the past two decades. With the widespread infection of MCPyV and the increase in MCC diagnoses, it is critical to better understand the biology of MCPyV and its oncogenic potential. In this chapter, we summarize recent discoveries regarding MCPyV molecular virology, host cellular tropism, mechanisms of MCPyV oncoprotein-mediated oncogenesis, and current therapeutic strategies for MCPyV-associated MCC. We also present epidemiological evidence for MCPyV infection in HIV patients and links between MCPyV and non-MCC human cancers.

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Detection of Merkel cell polyomavirus and human papillomavirus DNA in porocarcinoma

Cited by 16 publications

References 22 publications

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Clear Cell Porocarcinoma Arising in an Epidermodysplasia Verruciformis Patient

Merkel Cell Polyomavirus: A New DNA Virus Associated with Human Cancer

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