Detection of Metabolic Key Enzymes of Methane Turnover Processes in Cold Seep Microbial Biofilms

Wrede, Christoph; Krukenberg, Viola; Dreier, Anne; Reitner, Joachim; Heller, Christina; Hoppert, Michael

doi:10.1080/01490451.2012.665150

Cited by 13 publications

(19 citation statements)

References 68 publications

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“…On the surface, exposed to the sea water, a black layer consists mainly of aggregates between methane-oxidizing archaea of the ANME-2 group and sulfate reducing bacteria (SRB). SRB of this mat type often exhibit intracytoplasmic magnetosome-like chains of greigite precipitations [8, 13, 14]. Our results imply that greigite magnetosomes are one sink for (otherwise toxic) sulfides.…”

Section: Introductionmentioning

confidence: 75%

“…These samples have already been subjected to extended geochemical and structural analyses [9, 14]. Specific antibodies, directed against methyl-coenzyme M reductase (MCR), the key enzyme of (reverse) methanogenesis, were generated after purification of MCR as essentially described according to [15] by immunization of rabbits following established protocols (e. g., [16] and, the references therein).…”

Section: Methodsmentioning

confidence: 99%

“…Specific antibodies, directed against methyl-coenzyme M reductase (MCR), the key enzyme of (reverse) methanogenesis, were generated after purification of MCR as essentially described according to [15] by immunization of rabbits following established protocols (e. g., [16] and, the references therein). Specificity of the antibody was extensively studied for methanogenic archaea and reverse methanogens as already described [14, 16, 17]. …”

Section: Methodsmentioning

confidence: 99%

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Deposition of Biogenic Iron Minerals in a Methane Oxidizing Microbial Mat

Wrede

Kokoschka

Dreier

et al. 2013

Archaea

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The syntrophic community between anaerobic methanotrophic archaea and sulfate reducing bacteria forms thick, black layers within multi-layered microbial mats in chimney-like carbonate concretions of methane seeps located in the Black Sea Crimean shelf. The microbial consortium conducts anaerobic oxidation of methane, which leads to the formation of mainly two biomineral by-products, calcium carbonates and iron sulfides, building up these chimneys. Iron sulfides are generated by the microbial reduction of oxidized sulfur compounds in the microbial mats. Here we show that sulfate reducing bacteria deposit biogenic iron sulfides extra- and intracellularly, the latter in magnetosome-like chains. These chains appear to be stable after cell lysis and tend to attach to cell debris within the microbial mat. The particles may be important nuclei for larger iron sulfide mineral aggregates.

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Section: Introductionmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Deposition of Biogenic Iron Minerals in a Methane Oxidizing Microbial Mat

Wrede

Kokoschka

Dreier

et al. 2013

Archaea

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“…The anaerobic oxidation of methane is thought to be mediated by syntrophic consortia of methanotrophic Archaea and dissimilatory sulfate-reducing bacteria (140). Vibrioid, dissimilatory, sulfate-reducing MTB have been implicated as symbiotic partners in consortia responsible for the anaerobic oxidation of methane in black microbial mats associated with cold methane seep concretionary carbonate buildups in the anoxic sediment of the Black Sea (141,142). Immunogold labeling of these cells using an antibody specific to the dissimilatory adenosine-5=-phosphosulfate reductase (a key enzyme in dissimilatory sulfate reduction) ␤-subunit resulted in a strong positive signal (142).…”

Section: Magneticus)mentioning

confidence: 99%

“…Vibrioid, dissimilatory, sulfate-reducing MTB have been implicated as symbiotic partners in consortia responsible for the anaerobic oxidation of methane in black microbial mats associated with cold methane seep concretionary carbonate buildups in the anoxic sediment of the Black Sea (141,142). Immunogold labeling of these cells using an antibody specific to the dissimilatory adenosine-5=-phosphosulfate reductase (a key enzyme in dissimilatory sulfate reduction) ␤-subunit resulted in a strong positive signal (142). The magnetosome crystals, arranged in a chain within the cells, were shown to consist of an iron sulfide (141).…”

Section: Magneticus)mentioning

confidence: 99%

Ecology, Diversity, and Evolution of Magnetotactic Bacteria

Lefèvre

Bazylinski

2013

Microbiol Mol Biol Rev

376

388

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SUMMARY Magnetotactic bacteria (MTB) are widespread, motile, diverse prokaryotes that biomineralize a unique organelle called the magnetosome. Magnetosomes consist of a nano-sized crystal of a magnetic iron mineral that is enveloped by a lipid bilayer membrane. In cells of almost all MTB, magnetosomes are organized as a well-ordered chain. The magnetosome chain causes the cell to behave like a motile, miniature compass needle where the cell aligns and swims parallel to magnetic field lines. MTB are found in almost all types of aquatic environments, where they can account for an important part of the bacterial biomass. The genes responsible for magnetosome biomineralization are organized as clusters in the genomes of MTB, in some as a magnetosome genomic island. The functions of a number of magnetosome genes and their associated proteins in magnetosome synthesis and construction of the magnetosome chain have now been elucidated. The origin of magnetotaxis appears to be monophyletic; that is, it developed in a common ancestor to all MTB, although horizontal gene transfer of magnetosome genes also appears to play a role in their distribution. The purpose of this review, based on recent progress in this field, is focused on the diversity and the ecology of the MTB and also the evolution and transfer of the molecular determinants involved in magnetosome formation.

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Association of magnetotactic multicellular prokaryotes with Pseudoalteromonas species in a natural lagoon environment

Leão

Gueiros‐Filho

Bazylinski

et al. 2018

Antonie van Leeuwenhoek

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Magnetotactic bacteria, for the most part, are free-living, motile, unicellular prokaryotes that inhabit almost all marine and freshwater environments. One notable exception to the unicellular mode, however, are the magnetotactic multicellular prokaryotes. These morphologically unique prokaryotes (e.g., Candidatus Magnetoglobus multicellularis) are motile aggregates of 20-40 genetically identical, Gram-negative cells organised as a sphere (or ovoid in shape) and only motile as a unit. No specific close physical association between magnetotactic bacteria and non-magnetotactic microorganisms has ever been reported. Here, using culture-independent approaches, we show an unusual association between the spherical magnetotactic multicellular prokaryote Ca. Magnetoglobus multicellularis and Pseudoalteromonas species in environmental sediment and water samples collected from the Araruama Lagoon in Brazil. Cells of Pseudoalteromonas species were observed to be physically attached to the surface and, notably, even in the intercellular space of these spherical magnetotactic multicellular prokaryotes. An attempt to correlate the frequency of association between Pseudoalteromonas and magnetotactic multicellular prokaryotes with sediment depth was made but only a slight decrease in the number of Pseudoalteromonas cells per magnetotactic multicellular prokaryote was observed with increasing depth. Similar observations were made with magnetotactic multicellular prokaryotes from another Brazilian Lagoon (Rodrigo de Freitas) and the putative symbiont/parasite was detected. Although our results suggest some sort of specificity in the relationship between these prokaryotes, the precise nature of this association remains unclear.

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Detection of Metabolic Key Enzymes of Methane Turnover Processes in Cold Seep Microbial Biofilms

Cited by 13 publications

References 68 publications

Deposition of Biogenic Iron Minerals in a Methane Oxidizing Microbial Mat

Deposition of Biogenic Iron Minerals in a Methane Oxidizing Microbial Mat

Ecology, Diversity, and Evolution of Magnetotactic Bacteria

Association of magnetotactic multicellular prokaryotes with Pseudoalteromonas species in a natural lagoon environment

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