Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Meimani, Fatemeh; Nazari, Razieh; Rostami, Mahmoud Nateghi

doi:10.5812/zjrms.59559

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…World Health Organization (WHO) recently described multidrug-resistant (MDR) gram-negative bacilli like Pseudomonas aeruginosa and Acinetobacter baumannii as a severe health threat [40,41]. These bacteria are among ESKAPE organisms (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, A. baumannii, P. aeruginosa, Enterobacter) [42] and significant nosocomial pathogens [43][44][45]. Furthermore, the MDR problem in P. aeruginosa and A. baumannii limits their treatment of infections and is a challenge for physicians [46,47].…”

Section: Introductionmentioning

confidence: 99%

Effect of biosynthesized selenium nanoparticles using Nepeta extract against multidrug‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii

et al. 2022

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The problems of drug resistance in bacteria have become one of the daily challenges of the clinical treatment of patients, which inevitably forces us to use agents other than common antibiotics. Among these, we can take help from different properties and applications of nanoparticles (NPs). In this work, we evaluate the antibacterial activity of biosynthesized selenium nanoparticles (SeNPs) against standard strains of multidrug‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii. The production of biosynthesized SeNPs was proved by ultraviolet‐visible, Fourier transform infrared, X‐ray diffractometer, Field Emission Scanning Electron Microscopy, Dynamic light scattering, and Zeta potential methods. The cytotoxicity effect of SeNPs was investigated by MTT assay. Disk diffusion agar (DDA) and minimum inhibitory concentration (MIC) tests were performed on the mentioned bacteria using different classes of standard antibiotics and SeNPs separately. The impact of SeNPs combined with the desired antibiotics for better treatment of these infections was evaluated by checkerboard assay to determine the synergism effect. After the confirmation results based on the biosynthesis of SeNPs, both standard bacterial strains were susceptible to SeNPs and had a zone of inhibition using the DDA test. Also, the results of MICs showed that biosynthesized SeNPs in lower concentrations than antibiotics cause no growth of bacteria. On the other hand, according to the checkerboard assay, SeNPs had a synergistic effect with conventional antibiotics. The antibacterial sensitivity tests demonstrated the inhibition of bacterial growth in the presence of lower concentrations of SeNPs than common antibiotics. This property can be exerted in future applications to solve the drug resistance obstacle of microorganisms in bacterial diseases.

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Section: Introductionmentioning

confidence: 99%

Effect of biosynthesized selenium nanoparticles using Nepeta extract against multidrug‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii

et al. 2022

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Effect of lipopeptide extracted from Bacillus licheniformis on the expression of bap and luxI genes in multi-drug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa

Ghanipour,

Nazari,

Aghaei

et al. 2023

Amino Acids

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Enhancing the Antibacterial Impact of Lipopeptide Extracted from Bacillus licheniformis as a Probiotic against MDR Acinetobacter baumannii

Hosseini Bafghi,

Ghanipour,

Nazari

et al. 2024

Front. Biosci. (Landmark Ed)

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Background: The antibiotic resistance of microorganisms is escalating rapidly. Infections caused by opportunistic pathogens in immunocompromised individuals have prompted researchers to seek for potent and safe antibacterial agents. The purpose of this investigation was to explore the suppression of virulence gene expression, specifically the pga operon genes responsible in biofilm formation in Acinetobacter baumannii, through the utilization of metabolites obtained from probiotic bacteria. Methods: To assess the antimicrobial properties, standard strains of five probiotic bacteria were tested against a standard strain of multidrug-resistant (MDR) A. baumannii employing the agar gel diffusion technique. Following the identification of the most potent probiotic strain (Bacillus licheniformis), the existence of its LanA and LanM genes was confirmed using the polymerase chain reaction (PCR) test. High-performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR) techniques were employed to identify the intended metabolite, which was found to be a lipopeptide nature. The minimum inhibitory concentration (MIC) values and anti-biofilm activity of the targeted metabolite were determined using a dilution method in 96-well microplates and field emission scanning electron microscopy (FE-SEM). Real-time PCR (qPCR) was utilized for comparing the expression of pga operon genes, including pgaABCD, in A. baumannii pre- and post-exposure to the derived lipopeptide. Results: The MIC results indicated that the probiotic product inhibited the growth of A. baumannii at concentrations lower than those needed for conventional antibiotics. Furthermore, it was observed that the desired genes’ expression decreased due to the effect of this substance. Conclusions: This research concludes that the B. licheniformis probiotic product could be a viable alternative for combating drug resistance in A. baumannii.

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Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Cited by 3 publications

References 23 publications

Effect of biosynthesized selenium nanoparticles using Nepeta extract against multidrug‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii

Effect of biosynthesized selenium nanoparticles using Nepeta extract against multidrug‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii

Effect of lipopeptide extracted from Bacillus licheniformis on the expression of bap and luxI genes in multi-drug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa

Enhancing the Antibacterial Impact of Lipopeptide Extracted from Bacillus licheniformis as a Probiotic against MDR Acinetobacter baumannii

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