Detection of metallo-beta-lactamase producing Pseudomonas aeruginosa in intensive care units

Kali, Arunava; Srirangaraj, Sreenivasan; Kumar, Shailesh; Divya, Hema A; Kalyani, Akhila; Umadevi, S.

doi:10.4066/amj.2013.1824

Cited by 33 publications

(33 citation statements)

References 24 publications

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“…33(33%) being in the 51-60 years of age, 29(29%) in between the age of 41-50 years and 25(25%) in > 61 years of age. Diabetic foot complications manifests in myriad forms and in this study it was observed that 50(50%) were with ulcer foot, 22 (22)% patients were presented with cellulitis, 14(14%) with abscess, 10(10%) with gangrene of one or more toes, and 4(4%) with necrotizing fasciitis .Out of 100 samples processed 90(90%) were culture positive.Out of 90 culture positives, 89% (n=80) yielded monomicrobial growth and 11% (n=10) with polymicrobial growth. Out of 101 pathogens isolated, 70(69.30%) were Gram negative and 31(30.6%) were Gram positive.…”

Section: Results:-mentioning

confidence: 64%

Study of Bacterial Pathogens With Their Antibiogram Causing Diabetic Foot Ulcers.

sreeVaddadhi¹

2019

IJAR

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Section: Results:-mentioning

confidence: 64%

Study of Bacterial Pathogens With Their Antibiogram Causing Diabetic Foot Ulcers.

sreeVaddadhi¹

2019

IJAR

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“…Double Disk Synergy Test (DDST) and Combined Disc Test (CDT) are most commonly used to detect the MBL producing P. aeruginosa then Hodge test. Modified Hodge test detects only carbapenemase activity, which prevents the use of EDTA and therefore, does not confirm the metal dependence of the carbapenemase (Kali et al, 2013). Modified Hodge test, DDST and the CDT are easy, reliable, simple to perform and cheaper (Qu et al, 2009).…”

Section: Author(s) Agree That This Article Remains Permanently Open Amentioning

confidence: 99%

“…Placed imipenem (10 ug) disc in 20 mm center to center from a blank disc contained 10 µl of 0.5 M EDTA (750 ug) or EDTA disc (750 ug). The plate was incubated at 37°C for 16 to 18 h (Bhalerao et al, 2010;Chaudhari et al, 2011;Kali et al, 2013).…”

Section: Imipenem-edta Double-disc Synergy Test (Ddst)mentioning

confidence: 99%

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A study on metallo--lactamases producing Pseudomonas aeruginosa in water samples from various parts of Malaysia

Suryadevara¹,

O²,

Paulraj³

2017

Afr. J. Biotechnol.

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Pseudomonas aeruginosa is a Gram negative aerobic rod shaped bacterium and is an opportunistic pathogen that usually causes nosocomial infection in immunocompromised patient with various infections and affects normal healthy human as well. P. aeruginosa is also an omnipresent pathogen that can be inhabited in soil, water, vegetable, human and animal. Metallo-β-lactamases (MBL) producing P. aeruginosa are known to be resistant to almost the entire anti-pseudomonas agent via mechanism of low outer membrane permeability, β-lactamases synthesis and the efflux systems. This study was conducted to detect the potential of metallo-β-lactamases producing P. aeruginosa presence in water samples from various parts of Malaysia. In this study, 52 water samples were collected from various parts of Malaysia. These P. aeruginosa isolates were processed to these phenotypic methods, Hodge test which is used to detect the carbapenemase production, Imipenem-EDTA combined disc test (CDT). Imipenem-EDTA double disc synergy test (DDST) was used to determine metallo-betalactamases producing P. aeruginosa. Among 52 various sources of water samples, 13 water samples had positive P. aeruginosa isolates. 6 out of 13 positive isolates have shown positive results in Hodge test, CDT and DDST. All metallo-β-lactamases producing isolates are multi-drug resistant. Among water samples from various parts of Malaysia, MBL producing P. aeruginosa are highly found in Selangor followed by KL, and this P. aeruginosa are detected from drain followed by river. Although some advantages and disadvantages exist among these phenotyping methods, still CDT and DDST are optimal to detect MBL.

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“…Nazmul et al/ Detection Of Metallo-Β-Lactamases 292| International Journal of Pharmaceutical Research |January-March 2019 | Vol 11 | Issue 1overnight incubation. The test outcome was considered as positive(Kali et al, 2013;Suryadevara et al, 2017) and the procedure was repeated twice to ensure the results.…”

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confidence: 99%

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

Fazlul¹,

Najnin²,

Yasmin³

et al. 2019

IJPR

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The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-β-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clinical isolates of E. coli. A total of 65 E. coli isolates were collected from various clinical samples of Malaysian patients with bacterial infections. The conventional microbiological test was performed for isolation and identification of E. coli producing MBLs strains in this vicinity. Multidrug Resistance (MDR) of E. coli isolates were assessed using disk diffusion test. Phenotypic methods, as well as genotypic-PCR methods, were performed to detect the presence of metallo-β-lactamase resistance genes (blaIMP, blaVIM) in imipenem resistant strains. Out of 65 E. coli isolates, 42 isolates (57.3%) were MDR. The isolates from urine (19) produced significantly more MDR (10) isolates than other sources. Additionally, 19 (29.2%) imipenemresistant E. coli isolates contained 10 MBLs gene, 7(36.8%) isolates contained blaIMP and 3(15.7%) isolates contained blaVIM genes. This study revealed the significant occurrence of MBL producing E. coli isolates in clinical specimens and its association with health risk factors indicating an alarming situation in Malaysia. It demands an appropriate concern to avoid failure of treatments and infection control management.

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Detection of metallo-beta-lactamase producing Pseudomonas aeruginosa in intensive care units

Abstract: MBL production was the main resistance mechanism in the 11 carbapenem resistant P. aeruginosa isolates collected, with multidrug resistance associating significantly with MBL production in P. aeruginosa from our institution.

Cited by 33 publications

References 24 publications

Study of Bacterial Pathogens With Their Antibiogram Causing Diabetic Foot Ulcers.

Study of Bacterial Pathogens With Their Antibiogram Causing Diabetic Foot Ulcers.

A study on metallo--lactamases producing Pseudomonas aeruginosa in water samples from various parts of Malaysia

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

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