Detection of metallo-β-lactamase–producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran

Khosravi, Azar Dokht; Mihani, Fatemeh

doi:10.1016/j.diagmicrobio.2007.08.003

Cited by 80 publications

(69 citation statements)

References 19 publications

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“…by tourists). The most and also the sole frequent reported MBL-producing P. aeruginosa in Iran is blaVIM1 which is previously reported by several researchers (3,16,21,(27)(28)(29) in different cities of Iran, for example, in Ahvaz (south-west of Iran), Tehran (north of Iran), and Arak.…”

Section: Discussionsupporting

confidence: 52%

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Sarhangi

Motamedifar

Sarvari

2013

Jundishapur J Microbiol

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Background: Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa is responsible for many important nosocomial outbreaks including pneumonia and septicemia. Various studies have reported the separation of P. aeruginosa producing MBLs enzymes resulted in increases in multiple traditional antibiotics resistance including carbapenems, cephalosporins and penicillins. Objectives: In this study, based on the standard phenotypic and genotypic methods, we examined the MBLs possible production of a P. aeruginosa isolate. The main objective was exploring the dissemination of resistant MBL P. aeruginosa in south-west of Iran, Shiraz. Materials and Methods: During a six-month period-from October 2011 to March 2012-240 P. aeruginosa isolates, collected from four teaching hospitals in Shiraz located in southwest of Iran, were examined. The isolates were mainly collected from wound, urine, and sputum. Minimum inhibitory concentration (MIC) ≥4 µg/mL to imipenem was determined by micro-dilution broth. Identification of P. aeruginosa with MBL was detected using double disk synergy test (DDST) and polymerase chain reaction (PCR) using specific primers for blaIMP1, blaVIM2, blaSIM1, blaSPM1. All laboratory procedures was according to clinical and laboratory standards institute (CLSI) recommendations. Results: From 240 P. aeruginosa isolates, 82 (34.16%) isolates were imipenem-resistant (minimum inhibitory concentration (MIC) ≥4 µg/ mL). Among these imipenem-resistant isolates, 19 (23.3%) MBL-producing P. aeruginosa isolates were screened using DDST. A specific PCR test confirmed the presence of 18 (21.95%) P. aeruginosa producing blaIMP1 and blaVIM2. Conclusions: Beside our study, the detection of MBL genes were reported in a few studies conducted in Iran. The spread of detected MBLs producing P. aeruginosa were unprecedented in the region due to the lack of independent related researches or the novel incidence of these genes. This detection must be noted by associated clinical and health care services.

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Section: Discussionsupporting

confidence: 52%

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Sarhangi

Motamedifar

Sarvari

2013

Jundishapur J Microbiol

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“…In MDR bacteria, the over-expression of efflux pumps that expel structurally-unrelated drugs, contributes to the reduced susceptibility, by decreasing the intracellular concentration of antibiotics (21). Active efflux is now recognized as an important component of bacterial resistance to most of classes of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Khosravi

Mohammadian

2016

Jundishapur J Nat Pharm Prod

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Background: The emergence of highly drug resistant Pseudomonas aeruginosa in burn wounds is becoming a challenging problem for infection control programs. Today, it has been shown that antibiotic resistance in P. aeruginosa is the result of synergism between membrane permeability and multidrug resistance (MDR) efflux pumps. Objectives: The aim of this study was to investigate MDR and pan-drug resistance (PDR) in P. aeruginosa and detection of the presence of efflux pump MexAB genes by the polymerase chain reaction (PCR) technique. Materials and Methods: One-hundred and fifty P. aeruginosa were isolated from burn and wound infections. The isolates were confirmed using conventional culture and biochemical tests. Antibiotic resistance was evaluated using agar disk diffusion and broth micro dilution tests. For detection of efflux pump MexAB genes, the PCR technique with subsequent sequencing was used. Results: In total, 99 strains (66%) were MDR and one strain (0.667%) was PDR as detected by traditional susceptibility tests. The MDR isolates belonged to 53 burn (70.66%) and 46 wound (61.33%) infections. The PDR was only seen in one isolate from a burn strain (1.33%). The PCR technique revealed that all the 99 MDR strains and one PDR strain, contained MexA and MexB genes, representing an exhibitive intrinsic existence of these genes in MDR and PDR of P. aeruginosa. Conclusions: This study represented an increasing rate of MDR P. aeruginosa in burn and wound samples. Efflux MexAB genes were detected in all MDR and PDR strains. The P. aeruginosa strain isolated from burn cases showed higher drug resistance and PDR resistance was only noted in a burn sample.

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“…[23][24][25] VIM type was also highly prevalent in Turkey. 26 In previous studies in Egypt and Taiwan, VIM-2 demonstrated the highest outbreak among imipenem-resistant P. aeruginosa strains. 1,27 Since most of the integrones harbored VIM-1 gene code for aminoglycosides destructing enzymes, the matter is of clinical importance.…”

Section: Discussionmentioning

confidence: 99%

Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates

Amini¹,

Mobasseri²

2017

Int J Basic Sci Med

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Detection of metallo-β-lactamase–producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran

Cited by 80 publications

References 19 publications

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Dissemination of Pseudomonas aeruginosa Producing blaIMP1, blaVIM2, blaSIM1, blaSPM1 in Shiraz, Iran

Efflux MexAB-Mediated Resistance in Multidrug and Pan-Drug Resistant Strains of Pseudomonas aeruginosa Isolated From Patients With Burn and Wound Infections

Detection Rate of Metallo-β-Lactamase-Expressing Genes; blaVIM-1, blaVIM-2 and blaSPM-1 in Pseudomonas aeruginosa Isolates

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