Detection of methicillin-resistant staphylococci by biosensor assay consisting of nanoscale films on optical fiber long-period gratings

Bandara, Aloka B.; Zuo, Ziwei; Ramachandran, Siddharth; Ritter, Andreas; Heflin, James R.; Inzana, Thomas J.

doi:10.1016/j.bios.2015.03.041

Cited by 49 publications

(33 citation statements)

References 47 publications

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“…What is more, the sensitivity and sensitive range of this LPFG sensor to RI can continue to be improved and adjusted by coating nanofilm on the surface of the LPFG [10,15,23,24,25,26]. The kinds of LPFGs with a period of 180 µm and red-shifted resonant peaks are extremely conducive to applications in physical, biological, and chemical sensing due to their overwhelmingly high RI sensitivity.…”

Section: Resultsmentioning

confidence: 99%

An Ultrasensitive Long-Period Fiber Grating-Based Refractive Index Sensor with Long Wavelengths

Zhang

Shi

et al. 2016

Sensors

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The response of a novel long-period fiber grating (LPFG) with a period of 180 µm to a surrounding refractive index (RI) was investigated. The results displayed that, with the increase in RI of the surrounding media of cladding glass in the grating region, the resonant peak located at 1336.4 nm in the transmission spectrum gradually shifts towards a shorter wavelength, while the resonant peak located at 1618 nm gradually shifted towards a longer wavelength. Moreover, the resonant peak at 1618 nm is much more sensitive to the surrounding RI than that of the one at 1336.4 nm. Compared with the conventional LPFG and other types of wavelength-interrogated RI sensors, such as ring resonators, surface plasmon resonance sensors, and Fabry–Perot interferometric sensors, this novel LPFG possesses a higher sensitivity, which achieved 10,792.45 nm/RIU (RI unit) over a RI range of 1.4436–1.4489.

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Section: Resultsmentioning

confidence: 99%

An Ultrasensitive Long-Period Fiber Grating-Based Refractive Index Sensor with Long Wavelengths

Zhang

Shi

et al. 2016

Sensors

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“…Technical details of fabrication of the ISAM films and the NOFS operation have been described. 3,34 The ISAM method involves the alternate dipping of a charged substrate (optical fiber) into an aqueous solution of a polycation and then an aqueous solution of a polyanion at room temperature ( Supplementary Fig. 1).…”

Section: Fabrication Of the Isam Filmmentioning

confidence: 99%

Identification of Histophilus somni by a nanomaterial optical fiber biosensor assay

et al. 2018

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Histophilus somni is an opportunistic pathogen responsible for respiratory and systemic diseases of cattle and sheep. Rapid and accurate detection of H. somni is essential to distinguish H. somni from other potential pathogens for proper control and treatment of infections. Nanomaterial optical fiber biosensors (NOFS) recognize analyte interactions, such as DNA hybridization, with high specificity and sensitivity, and were applied to detect H. somni DNA in culture and clinical samples. An ionic self-assembled multilayer (ISAM) film was fabricated on a long-period grating optical fiber, and a biotinylated, nucleotide probe complementary to the H. somni 16S rDNA gene was coupled to the ISAM film. Exposure of the ISAM::probe to ≥100 killed cells of H. somni strain 2336 without DNA amplification resulted in attenuation of light transmission of ≥9.4%. Exposure of the complexed fiber to Escherichia coli or non-H. somni species of Pasteurellaceae reduced light transmission by ≤3.4%. Exposure of the ISAM::probe to blood, bronchoalveolar fluid, or spleen from mice or calves infected with H. somni resulted in ≥24.3% transmission attenuation. The assay correctly detected all 6 strains of H. somni tested from culture, or tissues from 3 separate mice and calves tested in duplicate. Six heterologous strains (representing 6 genera) reacted at below the cutoff value of 4.87% attenuation of light transmission. NOFS detected at least 100 H. somni cells without DNA amplification within 45 min with high specificity. Although different fibers could vary in signal sensitivity, this did not affect the sensitivity or specificity of the assay.

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“…; Bandara et al . ), the distinction between MSSA and MRSA strains thanks to an IgG recognizing PBP2a is presented. The authors used respectively SPR and optical fibre‐based read‐out as transducing techniques.…”

Section: Whole‐cell Detection With Biosensors Dedicated To S Aureusmentioning

confidence: 99%

“…Besides, there is no control experiment with a nonspecific antibody which would assess the importance of protein A interactions with the Fc region. Similarly, in two papers (Tawil et al 2013;Bandara et al 2015), the distinction between MSSA and MRSA strains thanks to an IgG recognizing PBP2a is presented. The authors used respectively SPR and optical fibre-based read-out as transducing techniques.…”

Section: Immunoglobulinsmentioning

confidence: 99%

On the challenges of detecting wholeStaphylococcus aureuscells with biosensors

Templier

Roupioz

2017

J Appl Microbiol

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SummaryDue to the increasing number of nosocomial infections and multidrug-resistant bacterial strains, Staphylococcus aureus is now a major worldwide concern. Rapid detection and characterization of this bacterium has become an important issue for biomedical applications. Biosensors are increasingly appearing as low-cost, easy-to-operate and fast alternatives for rapid detection. In this review, we will introduce the main characteristics of S. aureus and will focus on the interest of biosensors for a faster detection of whole S. aureus cells. In particular, we will review the most promising strategies in the choice of ligand for the design of selective and efficient biosensors. Their specific characteristics as well as their advantages and/or disadvantages will also be commented.

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Detection of methicillin-resistant staphylococci by biosensor assay consisting of nanoscale films on optical fiber long-period gratings

Cited by 49 publications

References 47 publications

An Ultrasensitive Long-Period Fiber Grating-Based Refractive Index Sensor with Long Wavelengths

An Ultrasensitive Long-Period Fiber Grating-Based Refractive Index Sensor with Long Wavelengths

Identification of Histophilus somni by a nanomaterial optical fiber biosensor assay

On the challenges of detecting wholeStaphylococcus aureuscells with biosensors

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