Detection of Methicillin-resistant Staphylococcus aureus using Chromogenic Agar and their Antimicrobial Susceptibility Pattern

Bhoi, Priyadarshini; Swain, Bichitrananda; Otta, Sarita

doi:10.31782/ijcrr.2021.13430

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…This study revealed that, cefoxitin disc diffusion and VITEK 2 system correctly identify 45 out of 46 as MRSA with sensitivity of 97.8%, specificity of 93.3% and accuracy of 95.6%, which in agreement with previous studies. [4,5,32] The benefit of VITEK 2 over disc diffusion test is that disc diffusion test requires less incubation time. CHROM agar correctly identify 42 out of 46 as MRSA with 91.3% sensitivity, 80% specificity and 85.7% accuracy, which was lower than that reported by Madhavan et al 3 who showed that chrome agar sensitivity was 100 % and near its specificity which was 78.6% and similar to Elzorkany et al 19 and Bhoi et al 32 In the present study, SCCmec typing by PCR revealed that the most predominant type (in both patients and HCWs) was type III and I, and the less prevalent types was II and V. So, hospital acquired MRSA (36 out of 46 sample) was more than community acquired MRSA (10 out of 46 samples).…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Awad

ElAziz

adwey

et al. 2022

Egyptian Journal of Medical Microbiology

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Background: Methicillin Resistant Staphylococcus aureus (MRSA) is a serious pathogen in hospitals leading to failure of treatment. MRSA colonization in pediatric patients increases the risk of severe invasive infections, while in the hospital and once they leave. Objectives: We aimed to study the molecular characters of MRSA and its prevalence among pediatric patients and healthcare workers (HCWs) in National Liver Institute (NLI), also the risk factors and mupirocin efficiency in MRSA decolonization were evaluated. Methodology: Nasal and hand swab specimens were obtained from patients and HCWs in pediatric department of NLI. MRSA detection was achieved by phenotypic methods such as cefoxitin diffusion test, VITEK2 compact system and chromogenic media, then confirmed by PCR. Susceptibility of S. aureus to different antibiotics was done by VITEK2 compact system. All isolated MRSA were subjected to SCCmec typing by multiplex PCR. Results: MRSA prevalence among HCWs was 18.9%, and among ward patients, PICU and NICU was 24.2 %, 16.7 % and 36.4 % respectively. Skin infections and less hygiene training were significant risk factors for colonization with MRSA among HCWs. Regarding antibiogram, high resistance rate was detected among MRSA isolates. Sensitivity and specificity of cefoxitin disc diffusion and VITEK2 were 97.8% and 93.3%, while that of CHROM agar was 91.3% and 80%. According to SCCmec typing, the most predominant SCCmec genotypes in the studied subjects was type III followed by type I then type IV, and the least prevalent was type V in both patients and HCWs. So, hospital acquired MRSA (36 /46) was more than community acquired MRSA strains (10/46 samples). When apply intranasal mupirocin ointment 2% regimen, a high successful decolonization rate was achieved. Conclusion: The most predominant type of SCCmec gene was type III followed by type I, which is linked to hospital acquired strains. Implementation of urgent antibiotic policy and strict infection control programs, in addition to mupirocin decolonization measures will decrease the transmission and spread of these multi drug resistant pathogens.

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Section: Discussionmentioning

confidence: 99%

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Awad

ElAziz

adwey

et al. 2022

Egyptian Journal of Medical Microbiology

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“…The use of selective media and chromogenic agar media containing cefoxitin may be useful for the identification of Staphylococcus aureus and MRSA. It has been suggested that the cefoxitin disk diffusion method has higher sensitivity and specificity for the detection of MRSA than the Chrom agar MRSA method [4].…”

Section: Introductionmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus (MRSA) in Dairy Products and Bulk-Tank Milk (BTM)

González-Machado,

Capita,

Alonso-Calleja

2024

Antibiotics

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In order to contribute to an assessment of the role of food in the risks of transmission of methicillin-resistant Staphylococcus aureus (MRSA), a review was undertaken of research on this microorganism in milk and dairy products published from January 2001 to February 2024. A total of 186 publications were selected, 125 for dairy products and 61 for bulk-tank milk (BTM). MRSA was detected in 68.8% of the research into dairy products and 73.8% of investigations relating to BTM, although in most studies the prevalence was less than 5%. Of the set of S. aureus strains isolated, approximately 30% corresponded to MRSA. The foods most extensively contaminated with this microorganism were raw milk and some types of soft cheese. Determination of the mecA gene on its own is known not to suffice for the detection of all MRSA strains. The great diversity of techniques used to study MRSA in milk and dairy products made it difficult to draw comparisons between studies. It would thus be advisable to develop a standardized protocol for the study of this microorganism in foods.

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Detection of Methicillin-resistant Staphylococcus aureus using Chromogenic Agar and their Antimicrobial Susceptibility Pattern

Cited by 2 publications

References 5 publications

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Methicillin-Resistant Staphylococcus aureus (MRSA) in Dairy Products and Bulk-Tank Milk (BTM)

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