Detection of methicillin-resistant Staphylococcus aureus in dairy cow farms

Visciano, Pierina; Pomilio, Francesco; Tofalo, Rosanna; Sacchini, Lorena; Saletti, Maria Antonietta; Tieri, Elga Ersilia; Schirone, Maria; Suzzi, Giovanna

doi:10.1016/j.foodcont.2014.06.022

Cited by 20 publications

(23 citation statements)

References 38 publications

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“…The higher frequency of resistance to beta-lactams, mainly ampicillin and penicillin G, in the tested S. aureus and CoNS isolates is consistent with previous studies on antimicrobial resistance patterns of staphylococci from the dairy industry [24]. This resistance profile has been associated with the common use of these drugs to prevent and treat infections in farm cattle [25].…”

Section: Discussionsupporting

confidence: 89%

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Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants

Lira

Givisiez

Sousa

et al. 2016

J Infect Dev Ctries

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Introduction: Biofilm-associated antimicrobial resistance is of increasing importance to the maintenance and spread of foodborne pathogens in the food industry. This study aimed to investigate the ability to form biofilm and the antimicrobial resistance of staphylococci contaminating small-scale goat milk dairy plants.Methodology: Sixty isolates were tested for antimicrobial resistance against 20 drugs by the microdilution method. Biofilm-forming traits were assessed by the microtiter plate method (MtP), Congo red agar method (CRA), and icaD gene detection by polymerase chain reaction (PCR). Results: High antimicrobial resistance to ampicillin (60/60; 100%), penicillin G (21/60; 35%), and erythromycin (15/60; 25%) was observed, but all isolates were susceptible to amoxicillin/K-clavulanate, ceftriaxone, ciprofloxacin, gentamicin, levofloxacin, linezolid, and moxifloxacin. No resistance to oxacillin or vancomycin was seen among Staphylococcus aureus. Twenty-seven isolates (27/60; 45%) were considered to form biofilm according to MtP, and similar biofilm-producing frequencies were observed in coagulase-negative staphylococci (CoNS) (20/44; 45.4%) and S. aureus (7/16; 43.7%). The icaD gene was observed only in S. aureus isolates. There was no association between biofilm production and antimicrobial resistance. A higher frequency of biofilm-producing staphylococci was found in isolates from bulk tank milk and hand swabs. On the other hand, isolates from pasteurized milk showed lower frequency of biofilm formation. Conclusions: Staphylococci contaminating goat dairy plants are potential biofilm producers. The results suggest no association between the ability to form biofilm and antimicrobial resistance.

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Section: Discussionsupporting

confidence: 89%

“…This resistance profile has been associated with the common use of these drugs to prevent and treat infections in farm cattle [25]. The absence of vancomycin or oxacillin resistance in S. aureus isolates is interesting, since resistance to these antibiotics has been increasingly detected in isolates from farms and foods [24,26,27].…”

Section: Discussionmentioning

confidence: 99%

Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants

Lira

Givisiez

Sousa

et al. 2016

J Infect Dev Ctries

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“…The emergence of LA-MRSA has led to concerns about its transmission via the food chain. In recent years, LA-MRSA has frequently been detected in food-producing animals (Wagenaar et al, 2009 ; Petinaki and Spiliopoulou, 2012 ; Visciano et al, 2014 ), meat (Rodríguez-Lázaro et al, 2015 ), milk and dairy products (Song et al, 2015 ), fish (Hammad et al, 2012 ), and ready-to-eat (RTE) food products (Hammad et al, 2012 ; Wang et al, 2014 ), causing a significant public health concern.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Retail Ready-to-Eat Foods in China

Yang

Zhang

et al. 2016

Front. Microbiol.

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Staphylococcus aureus, particularly methicillin-resistant S.aureus (MRSA), is a life-threatening pathogen in humans, and its presence in food is a public health concern. MRSA has been identified in foods in China, but little information is available regarding MRSA in ready-to-eat (RTE) foods. We aimed to investigate the prevalence of S. aureus and MRSA in Chinese retail RTE foods. All isolated S. aureus were tested for antimicrobial susceptibility, and MRSA isolates were further characterized by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. Of the 550 RTE foods collected from 2011 to 2014, 69 (12.5%) were positive for S. aureus. Contamination levels were mostly in the range of 0.3–10 most probable number (MPN)/g, with five samples exceeding 10 MPN/g. Of the 69 S. aureus isolates, seven were identified as MRSA by cefoxitin disc diffusion test. Six isolates were mecA-positive, while no mecC-positive isolates were identified. In total, 75.8% (47/62) of the methicillin-susceptible S. aureus isolates and all of the MRSA isolates were resistant to three or more antibiotics. Amongst the MRSA isolates, four were identified as community-acquired strains (ST59-MRSA-IVa (n = 2), ST338-MRSA-V, ST1-MRSA-V), while one was a livestock-associated strain (ST9, harboring an unreported SCCmec type 2C2). One novel sequence type was identified (ST3239), the SCCmec gene of which could not be typed. Overall, our findings showed that Chinese retail RTE foods are likely vehicles for transmission of multidrug-resistant S. aureus and MRSA lineages. This is a serious public health risk and highlights the need to implement good hygiene practices.

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“…Staphylococcus aureus is a pathogen bacteria for both human and animals and causes multiple disease processes including skin and soft tissue infections, sepsis, osteomyelitis, and pneumonia (Visciano et al, ). The first report of methicillin‐resistant S. aureus (MRSA) changed the status of this pathogen as an emerged nosocomial agent, and it has been associated with healthcare‐acquired (HA‐MRSA) infections.…”

Section: Introductionmentioning

confidence: 99%

Investigation of mecA gene, virulence traits and antibiotic resistance profiles in methicillin‐resistant Staphylococcus aureus isolates from dairy products

Muş

Çetinkaya

Karahan

et al. 2019

Journal of Food Safety

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This study was performed to determine the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in raw milk and dairy products and the presence of virulence traits in the isolates obtained and to assess their resistance to several antibiotics. MRSA was detected by CHROMagar MRSA II in 148 (22.8%) of 650 samples. Ninety‐eight isolates were confirmed as MRSA, 86 of these isolates were phenotype‐positive, 9 were phenotype/mecA‐positive, and 3 were phenotype‐negative but mecA‐positive. However, the virulence genes were not found in any MRSA strains. According to the results of antibiotic susceptibility tests, 100, 44.9, and 29.6% of strains were resistant to penicillin, tetracycline, and clindamycin, respectively. A low prevalence (1.02%) of resistance was observed against vancomycin and chloramphenicol antibiotics. Furthermore, multidrug resistance was seen among MRSA strains isolated from cheese, butter and buttercream samples. In contrast with other studies, we identified a high‐level vancomycin‐resistant MRSA strain in one buttercream sample and the intermediate‐level vancomycin resistance in six different multiple‐resistant MRSA strains from food of dairy origin. Practical applications Recently, MRSA has been isolated from foods of animal origin and become one of the rising public health concerns worldwide. This study focused on the prevalence of MRSA in raw milk and dairy products, the presence of genes encoding virulence factors that allow it to cause the disease and their antibiotic susceptibility patterns. Consequently, we revealed the emergence of resistance to vancomycin and multiple antibiotics among MRSA strains. The isolation of high‐level vancomycin‐resistant MRSA strain is an important finding because vancomycin continues to be the first‐line treatment agent for MRSA infections.

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Detection of methicillin-resistant Staphylococcus aureus in dairy cow farms

Cited by 20 publications

References 38 publications

Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants

Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants

Prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Retail Ready-to-Eat Foods in China

Investigation of mecA gene, virulence traits and antibiotic resistance profiles in methicillin‐resistant Staphylococcus aureus isolates from dairy products

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