Detection of methicillin resistant staphylococcus aureus using mec a, ribotyping and antibiogram profile of Pakistani clinical isolates

Fatima, Adeela; Sajid, Imran; Riaz, Saba; Saeed, Muhammad

doi:10.29309/tpmj/2019.26.06.3614

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…These results are similar to our results in which high susceptibility was found against vancomycin (100%) and linezolid (98%) followed by clindamycin (81%) and rifampicin (77.3%). 4 While another study conducted in Saudi Arabia showed slight different results reporting 60% resistance against penicillin while high susceptibility for clindamycin (85%). 22…”

Section: Discussionmentioning

confidence: 96%

Multidrug resistant Staphylococcus aureus; its incidence and antibiotic sensitivity pattern.

Hassan¹,

Farva²,

Bhutta³

2021

TPMJ

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Objective: To determine incidence of MRSA in our community and its antibiotic susceptibility pattern. Study Design: Cross Sectional study. Setting: Department of Pathology, Sahara Medical College Narowal. Period: January 2020 to June 2020. Material & Methods: Bacterial isolates were taken from the samples of blood, pus and other body fluids sent to the hospital laboratory to determine culture and sensitivity pattern. Those samples positive for staphylococcus aureus were included in the study for further examination. MRSA were detected using conventional technique (catalase, coagulase and DNase methods) and confirmed by phenotypic and molecular characterization techniques (cefoxitin and oxacillin sensitivity, mec-A gene and 16S rRNA genes sequencing methods). Their antibiotic sensitivity was determined using standard Kirby Bauer disc diffusion technique. Chi square test was applied on collected data using SPSS-24. Results: Total 158 bacterial isolates containing staphylococcus aureus were included in the study. MRSA (multi drug resistant staphylococcus aureus) was detected in 33.5% samples and MSSA (multi drug sensitive staphylococcus aureus) was detected in 66.5% samples. Frequency of MSSA was more than MRSA. Male gender was more affected (60%) than female (40%). All bacterial isolates containing staphylococcus aureus were resistant to oxacillin and cefoxitin. MRSA isolates were sensitive to vancomycin, linezolid, teicoplanin, fosfomycin and fusidic acid, rifampicin, clindamycin, minocyclin and chloramphenicol, while resistant to penicillin, azithromycin, ciprofloxacin, erythromycin and co-trimoxazole. Conclusion: MRSA is detected by conventional technique followed by phenotypic and molecular characterization methods for confirmation. Methicillin sensitive staphylococcus aureus was detected more commonly than methicillin resistant staphylococcus aureus in our study sample. Proper culture and antibiotic sensitivity pattern is necessary to deal with MRSA.

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Section: Discussionmentioning

confidence: 96%

Multidrug resistant Staphylococcus aureus; its incidence and antibiotic sensitivity pattern.

Hassan¹,

Farva²,

Bhutta³

2021

TPMJ

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“…This combined pattern of resistance with MRSA signals towards presence of gyr A, B mutations and erm genes along with mecA gene. Maximum sensitivity has been shown to glycopeptides and linezolid by some researchers [13,15,18].…”

Section: Discussionmentioning

confidence: 99%

Frequency and Sensitivity Patterns of Staphylococcus Aureus in a Tertiary Care Setting

Khawaja

Ejaz

Abeer

et al. 2021

JPRI

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Background: Staphylococcus aureus (SA) is a major etiological pathogen causing multiple infections and broadly known as a serious public health challenge faced due to antibiotic resistance. It is the need of time that infection prevention and control strategies; and antibiotic stewardship policies have to be employed conjointly to minimize the extended rise of antibiotic resistance. Objectives: To determine the frequency and sensitivity patterns of Staphylococcus aureus in tertiary care setting. Study Design: Descriptive study Place & Duration of Study: Pathology Laboratory of tertiary care center from 1st March’ 2020 till 28th February’ 2021. Materials & Methods: A total 643 Staph. aureus isolated from various clinical specimens received in a tertiary care hospital; were processed and identified by culture, staining and bench tests. Sensitivity testing was done by Disc Diffusion method. Resistance to cefoxitin(30µg) was labelled as Methicillin Resistant Staphylococcus aureus (MRSA). Constitutive and Inducible Clindamycin resistance was also evaluated. (CLSI, 2020-21). Results: During the study period 125 (19.44%) MRSA were recovered. Statistically, gender distribution regarding MRSA was significant, most of SA was recovered from blood (53.68%), while 46.31% from pus and wound swabs. The frequency of MRSA from Surgical and allied wards was higher (52.63%) than Medicine and allied wards (47.36%). Sensitivity to vancomycin, linezolid and tigecycline was noted 100% by all the isolates. Sensitivity to clindamycin and Doxycycline was 68.42% and 64.81% respectively; while resistance to erythromycin, ciprofloxacin and trimethoprim/sulfamethoxazole was 73.68%, 60% and 57.89%, respectively. Conclusion: The hazardous infections due to Staphylococcus aureus are worrisome in the present therapeutic scenario. A levelheaded prescription of sensitive antibiotics has to be ensured to minimize the rising frequency of resistant strains of SA.

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Detection of methicillin resistant staphylococcus aureus using mec a, ribotyping and antibiogram profile of Pakistani clinical isolates

Cited by 2 publications

References 9 publications

Multidrug resistant Staphylococcus aureus; its incidence and antibiotic sensitivity pattern.

Multidrug resistant Staphylococcus aureus; its incidence and antibiotic sensitivity pattern.

Frequency and Sensitivity Patterns of Staphylococcus Aureus in a Tertiary Care Setting

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