Detection of microbial contamination based on uracil-selective synthetic receptors

Bezděková, Jaroslava; Vodova, Milada; Dolezelikova, Kristyna; Zítka, Jan; Šmerková, Kristýna; Zítka, Ondřej; Adam, Vojtěch; Vaculovičová, Markéta

doi:10.1016/j.talanta.2020.121813

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…We recently proved that the bioanalytical devices designed and manufactured in this way are fully functional. 23 The main supporting structure (Fig. 4A(a)), which is also used for wiring protection, is constructed using an aluminum building kit.…”

Section: Resultsmentioning

confidence: 99%

Fully automated station for testing, characterizing and modifying screen-printed electrodes

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Fully automated station for testing, characterizing and modifying screen-printed electrodes

et al. 2022

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“…It is important to stress that this first-ever reported principle of detection of thiols rests in a unique in-situ UV light-induced formation of fluorescent QD nanoparticles developed in this research group 25 , 27 , and therefore is to a large degree an area with existing expertise. Similarly, polydopamine MIPs is also an area where our group has been active, and the materials were evaluated and characterized in our previous works 28 .…”

Section: Resultsmentioning

confidence: 99%

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

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et al. 2021

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In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12–150 µg/mL was linear with a detection limit of 3.6 µg/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.

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“…In MIT, a specific target molecule or a structural analogue (virtual template) was firstly employed to facilitate recognition site formation through covalent or non-covalent interaction with bulk phase by polymerization, after removing the template, a molecularly imprinted polymer (MIP) was subsequent achieved with imprinted cavities which highly match the three-dimensional shape, size and functional site of target molecule ( Gao et al, 2020 ; Pan et al, 2018 ; Si et al, 2018 ). Compared to other adsorption systems, MIPs exhibit three unique characteristics of structure predetermination, application universality and recognition specificity and are highly promising for solid phase extraction ( Zhang et al, 2020 ), chemical sensors ( Cheng et al, 2017 ; Lopez et al, 2021 ; Raziq et al, 2021 ), capillary electrophoresis ( Bezdekova et al, 2021 ), simulated antibodies ( Seitz et al, 2021 ) and chromatographic separation ( Cheng et al, 2014 ). However, some drawbacks were founded in traditional molecular imprinting technology with the deepening development, including the serious embedding and uneven distribution of imprinting sites, incomplete removal of template, poor selectivity, and slow mass transfer rate, which directly limited the practical application of MIPs ( Deng et al, 2012 ; Wulff 2002 ).…”

Section: Introductionmentioning

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Magnetic Surface Molecularly Imprinted Polymer for Selective Adsorption of 4-Hydroxycoumarin

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4-hydroxyl coumarin (HC), an important intermediate during the synthesis procedure of rodenticide and anti-cardiovascular drug, shows highly medicinal value and economic value. To achieve the efficient adsorption of HC from natural biological samples, a novel magnetic surface molecularly imprinted polymer (HC/SMIPs) was constructed by employing methacrylic acid (MAA) as functional monomer, organic silane modified magnetic particles as matrix carrier and HC as template molecule. Due to the numerous specific imprinted cavities on the HC/SMIPs, the maximum adsorption capacity of HC/SMIPs for 4-hydroxycoumarin could reach to 22.78 mg g−1 within 20 min. In addition, HC/SMIPs exhibited highly selective adsorption for 4-hydroxycoumarin compared with other active drug molecules (osthole and rutin) and showed excellent regeneration performance. After 8 cycles of adsorption-desorption tests, the adsorption capacity of HC/SMIPs just slightly decreased by 6.64%. The efficient selective removal and easy recycle of 4-hydroxycoumarin from biological samples by HC/SMIPs made a highly promising to advance the application of imprinting polymers in complex practical environments.

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Detection of microbial contamination based on uracil-selective synthetic receptors

Cited by 10 publications

References 44 publications

Fully automated station for testing, characterizing and modifying screen-printed electrodes

Fully automated station for testing, characterizing and modifying screen-printed electrodes

UV-induced Zn:Cd/S quantum dots in-situ formed in the presence of thiols for sensitive and selective fluorescence detection of thiols

Magnetic Surface Molecularly Imprinted Polymer for Selective Adsorption of 4-Hydroxycoumarin

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