Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an  Ion-Sensitive Field-Effect Transistor

Matsuura, Koji; Asano, Yuka; Yamada, Akira; Naruse, Keiji

doi:10.3390/s130202484

Cited by 29 publications

(12 citation statements)

References 26 publications

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“…Literature concerning biofilm‐formation of the other pathogens only detected by sonification is scarce. These rather rare pathogens definitely need to be further investigated in regards of biofilm formation …”

Section: Discussionmentioning

confidence: 99%

Sonication culture improves microbiological diagnosis of modular megaprostheses

et al. 2016

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Modular megaprostheses are known for high infection rates followed by high rates of revisions. Microbial biofilms growing adherently on prosthetic surfaces may inhibit the detection of the pathogens causing prosthetic joint infections. We sought to answer the following questions: Does sonication culture (SC) improve the microbiological diagnosis of periprosthetic infections of megaprostheses compared to conventional tissue culture (TC)? Which pathogens were detected on the surface of megaprostheses with either SC or TC and do the findings help to identify low-grade infections? Included were 31 patients with modular megaprostheses, whose implant had been explanted due to suspected joint infection or revision surgery. SCs were performed according to the protocol by Trampuz et al. The diagnosis of infection was evaluated according to the definition of the Musculoskeletal Infection Society. The sensitivity of SC was 91.3% compared to 52.2% for TC and the specificity was 100% for SC and TC (p = 0.004). Under preoperative antibiotic therapy, the sensitivity of SC was 83.3% while the sensitivity of TC was 50%. Without preoperative antibiotic therapy the sensitivity of SC was 100% compared to 54.5% for TC. In nine cases, SCs detected microorganisms, while TC was negative. Detected bacteria were Staphylococcus epidermidis in four, Micrococcus species in one, Finegoldia magna in one, Brevibacterium casei in one, Pseudomonas fluorescens in one, and Enterococcus faecium in one. SC is a reliable method for dislodging pathogens from orthopedic implants. The SC of modular megaprostheses showed significantly higher pathogen detection than the periprosthetic TC, especially for low virulence pathogens. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1383-1387, 2017.

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Section: Discussionmentioning

confidence: 99%

Sonication culture improves microbiological diagnosis of modular megaprostheses

et al. 2016

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“…Em relação aos alimentos, a água mineral envasada vem sendo motivo de preocupação pela fácil contaminação por Pseudomonas aeruginosa, cando sob responsabilidade da empresa envasadora a análise das características microbiológicas na fonte, poço e no produto nal (ANVI-SA Resolução nº 310/1999). Outra bactéria formadora de bio lme, encontrada, também, na amostra 1I, é a espécie Micrococcus luteus (Matsuura et al, 2013).…”

Section: Isolamento E Identi Cação Das Linhagens Bacterianasunclassified

Análise Microbiológica De Amostras De Amendoim Provenientes Do Mercado Público De Porto Alegre/Rs

2018

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O amendoim é uma leguminosa amplamente consumida apesar do grande risco de contaminação por fungos e bactérias. O objetivo deste estudo foi avaliar microbiologicamente a presença de coliformes totais, coliformes termotolerantes, bactérias mesófilas aeróbias, bolores e leveduras, em amostras de amendoim torrado vendidas no mercado público de Porto Alegre/RS, armazenadas de três formas diferentes. Os resultados indicaram ausência de fungos ou leveduras, mas bactérias mesófilas aeróbias foram encontradas em todos os tipos de armazenagem, sendo que, no dispensador gravitacional (1V), a contagem foi de 3,7 x 102UFC/g, seguida do recipiente comum (2-G) com 3,6 x 102UFC/g e, em menor quantidade, o industrializado (1I) com 2,7 x 102UFC/g. Coliformes totais foram encontrados nas mesmas amostras, 1V (2,4 x 102NMP/g), 2-G (1,3 x 102NMP/g) e 1I (1,1 x 101NMP/g). Foram isoladas dezoito bactérias, a maioria caracterizada como Gram-positiva e identificada a partir do sequenciamento do gene 16SrRNA. As bactérias patogênicas de importância em alimentos encontradas nas amostras de amendoim torrado analisadas foram Bacilluscereus, Pseudomonasaeruginosa e Staphylococcus sp., que podem ter vindo do não seguimento das boas práticas de manipulação e de falta de assepsia no armazenamento.

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“…We evaluated the effect of the turbulent method from the sensor signal directly. The sensor probe responded quickly (msec order) because the pH-sensitive region of the pH-FET used in the instrument was coated with Ta 2 O 5 [ 39 , 40 , 41 , 42 , 43 ]. This rapid response made it possible to monitor the transient characteristics of the measurement directly from the sensor signal.…”

Section: Resultsmentioning

confidence: 99%

A Simple Method for Decreasing the Liquid Junction Potential in a Flow-through-Type Differential pH Sensor Probe Consisting of pH-FETs by Exerting Spatiotemporal Control of the Liquid Junction

Yamada

Mohri

Nakamura

et al. 2015

Sensors

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The liquid junction potential (LJP), the phenomenon that occurs when two electrolyte solutions of different composition come into contact, prevents accurate measurements in potentiometry. The effect of the LJP is usually remarkable in measurements of diluted solutions with low buffering capacities or low ion concentrations. Our group has constructed a simple method to eliminate the LJP by exerting spatiotemporal control of a liquid junction (LJ) formed between two solutions, a sample solution and a baseline solution (BLS), in a flow-through-type differential pH sensor probe. The method was contrived based on microfluidics. The sensor probe is a differential measurement system composed of two ion-sensitive field-effect transistors (ISFETs) and one Ag/AgCl electrode. With our new method, the border region of the sample solution and BLS is vibrated in order to mix solutions and suppress the overshoot after the sample solution is suctioned into the sensor probe. Compared to the conventional method without vibration, our method shortened the settling time from over two min to 15 s and reduced the measurement error by 86% to within 0.060 pH. This new method will be useful for improving the response characteristics and decreasing the measurement error of many apparatuses that use LJs.

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Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor

Cited by 29 publications

References 26 publications

Sonication culture improves microbiological diagnosis of modular megaprostheses

Sonication culture improves microbiological diagnosis of modular megaprostheses

Análise Microbiológica De Amostras De Amendoim Provenientes Do Mercado Público De Porto Alegre/Rs

A Simple Method for Decreasing the Liquid Junction Potential in a Flow-through-Type Differential pH Sensor Probe Consisting of pH-FETs by Exerting Spatiotemporal Control of the Liquid Junction

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