Detection of microcystins with protein phosphatase inhibition assay, high-performance liquid chromatography–UV detection and enzyme-linked immunosorbent assay

Rapala, Jarkko; Erkomaa, Kirsti; Kukkonen, Jaana; Sivonen, Kaarina; Lahti, Kirsti

doi:10.1016/s0003-2670(02)00588-3

Cited by 182 publications

(151 citation statements)

References 39 publications

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“…Therefore, some phases like Oasis â HLB (Waters) and immunoaffinity columns were compared to C 18 cartridges, with better performances than the latter (Rapala et al 2002, Aranda-Rodriguez et al 2003). …”

Section: Resultsmentioning

confidence: 99%

Cyanobacterial occurrence and detection of microcystin by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil

Carvalho¹,

Sant’Anna²,

Gemelgo³

et al. 2007

Rev. bras. Bot.

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-(Cyanobacterial occurrence and detection of microcystins by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil). Billings and Guarapiranga Reservoirs were deeply affected by environmental disturbances, which more evident consequence are the cyanobacterial blooms. Microcystins are the most common cyanotoxin in freshwaters and more than 70 types are known. Different methods for microcystins analysis in water can be used, among which ELISA and HPLC are the most frequently employed. However, less sophisticated and more economic methods can also be used. This is the case of planar chromatography (thin-layer chromatography) method previously used in cyanotoxins purification but gradually replaced by others. Posterior optimization of the microcystin chromatography conditions and because of its simplicity, rapidity, efficiency and low cost, this method is again considered an option for the analysis of microcystins and nodularins. Considering the importance of Billings and Guarapiranga Reservoirs for drinking water supplies and the few scientific data about cyanobacteria and cyanotoxins in these water bodies, the aims of this work are to analyze the biodiversity of cyanobacteria in the Billings and Guarapiranga Reservoirs and the detection of dissolved microcystins in the water. It was possible to identify 17 species of cyanobacteria, 9 of them being potentially toxic. In Billings Reservoir Microcystis aeruginosa (Kützing) Kützing and Cylindrospermopsis raciborskii (Woloszynska) Seenayya & Subba Raju are the most common species, while in Guarapiranga Reservoir only M. aeruginosa was considered as a common species. Microcystins were detected in all Billings Reservoir samples and in only one sample from Guarapiranga Reservoir.Key words -cyanobacteria, drinking water, microcystins, planar chromatography, reservoirs RESUMO -(Ocorrência de cianobactérias e detecção por cromatografia planar de microcistinas dissolvidas nas águas superficiais das represas Billings e de Guarapiranga, SP, Brasil). As represas Billings e Guarapiranga foram profundamente afetadas por fatores ambientais cuja conseqüência mais evidente são as florações de cianobactérias. As microcistinas formam a classe de cianotoxinas mais freqüente em água doce e são comumente analisadas por ELISA ou CLAE. No entanto, processos menos sofisticados e mais econômicos também podem ser usados. Este é o caso da cromatografia planar (cromatografia em camada delgada), método anteriormente usado em trabalhos de purificação de cianotoxinas, mas que foi gradualmente substituído por outros. Assim, considerando a importância das represas Billings e Guarapiranga para o abastecimento público e a carência de informações científicas sobre as cianobactérias e as cianotoxinas que nelas ocorrem, nossos objetivos foram o estudo da composição desses organismos e a detecção de microcistina dissolvida na água. Foi possível identificar 19 espécies de cianobactérias, das quais 9 são consideradas potencialmente tóxicas. Microcystis aeruginosa...

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Section: Resultsmentioning

confidence: 99%

Cyanobacterial occurrence and detection of microcystin by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil

Carvalho¹,

Sant’Anna²,

Gemelgo³

et al. 2007

Rev. bras. Bot.

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“…Bioassays using mice [16] or plants (Sinapis alba L.) [17] have been used to screen for MCs, but were found to be non-specific or time-consuming. Biochemical methods such as enzyme-linked immunosorbent assay (ELISA) [18][19][20] and the protein phosphatase inhibition assay [5,21] are useful as screening methods due to their high sensitivity and high throughput, but they often have poor identification ability and the potential for false-positives. Reversedphase high-performance liquid chromatography (HPLC) with UV detection [20], diode array detection [22][23][24][25][26][27] or electrochemical detection [28], LC-mass spectrometry (LC-MS) [29][30][31], capillary electrophoresis (CE) [30], and CE-MS [30] have also been used for the identification and quantification of MCs.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Xiao

et al. 2009

Analytica Chimica Acta

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“…There have been many analytical methods for MC-LR, including protein phosphatase inhibition assay (PPIA) [15], enzyme-linked immunosorbent assay (ELISA) [16], liquid chromatography (LC) [17][18][19], capillary electrophoresis (CE) [20,21] and liquid chromatography-mass spectrometry (LC-MS) [22][23][24]. Because of its high specificity and sensitivity, liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become the method of choice for quantitative determination of analytes in biological samples [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography–tandem mass spectrometry

Dai

Xie

Gao

et al. 2008

Journal of Chromatography B

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Detection of microcystins with protein phosphatase inhibition assay, high-performance liquid chromatography–UV detection and enzyme-linked immunosorbent assay

Cited by 182 publications

References 39 publications

Cyanobacterial occurrence and detection of microcystin by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil

Cyanobacterial occurrence and detection of microcystin by planar chromatography in surface water of Billings and Guarapiranga Reservoirs, SP, Brazil

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Simultaneous determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography–tandem mass spectrometry

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