Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol

Pavlica, Mirjana; Klobučar, Göran; Vetma, Nataša; Erben, Radovan; Papeš, Dražena

doi:10.1016/s1383-5718(99)00222-3

Cited by 107 publications

(42 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a consequence, PCP has resulted in widespread environmental contamination in the local water environment where PCP has been detected in surface waters in China (up to 1.15 g/L) [20,25]. A high PCP concentration has been detected in Dongting Lake (up to 103.7 g/L), which is attributed to its application for the control of snailborne schistosomiasis [26][27][28]. Previous studies indicate that principal mechanisms of action of PCP-induced toxicity seem to be related to the uncoupling of oxidative phosphorylation in mitochondria and the generation of reactive oxygen species (ROS) [17,29].…”

Section: Introductionmentioning

confidence: 99%

Effect of titanium dioxide nanoparticles on the bioavailability, metabolism, and toxicity of pentachlorophenol in zebrafish larvae

Fang

Shi

Zhang

et al. 2015

Journal of Hazardous Materials

136

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Effect of titanium dioxide nanoparticles on the bioavailability, metabolism, and toxicity of pentachlorophenol in zebrafish larvae

Fang

Shi

Zhang

et al. 2015

Journal of Hazardous Materials

136

View full text Add to dashboard Cite

“…This is true especially for invertebrates, which represent more than 90% of the extant species, and play important roles in ecosystem functioning [Dixon et al, 2002]. Among aquatic invertebrates, the freshwater bivalve Dreissena polymorpha has been successfully used as a biological model for the evaluation of potential genotoxic effects in both field and laboratory experiments [Mersch and Beauvais, 1997;Pavlica et al, 2000;Woznicki, 2005;Le Goff et al, 2006;Pellacani et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of DNA damage induced by decabromodiphenyl ether (BDE‐209) in hemocytes of Dreissena polymorpha using the comet and micronucleus assays

Riva

Binelli

Cogni

et al. 2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

The recent widespread production and use of flame retardants, polybrominated diphenyl ethers (PBDEs), is one of the reason of the increasing contamination observed worldwide. At the present, deca-BDE mixture, in which the decabromodiphenyl ether (BDE-209) is the major congener (98%), dominates the EU market. The potential genotoxicity of BDE-209 was examined in the freshwater bivalve zebra mussel (Dreissena polymorpha) by means of Comet assay and micronucleus assay (MN assay). Mussels were exposed in vivo to BDE-209 at nominal concentration of 0.1, 2, and 10 mug/l under laboratory conditions. The assays were performed on the bivalve hemocytes monitoring the levels of DNA strand breaks and the percentage of micronuclei until 168 and 96 hr of exposure, respectively. At the same time, BDE-209 levels were measured daily in mussel soft tissues to evaluate the bioaccumulation. Results of the Comet assay showed a significant increase of DNA damages compared to controls, but a lack of dose/effect relationship probably due to the formation of less-brominated congeners. By contrast, no significant changes in MN frequency from baseline levels were observed. These preliminary results about the potential genotoxicity of this compound in invertebrates indicated a clear BDE-209 capability to induce DNA damage, but no irreversible effects on DNA hemocytes. Furthermore, bioaccumulation of this high-molecular-weight substance and its uptake mechanism in zebra mussel are also discussed.

show abstract

“…O teste de micronúcleos (MN), descrito no Item 5, foi aplicado com êxito em um grande número de estudos de campo que utiliza bivalves dos gêneros Mytilus (DOLCETTI; VENIER, 2002;VENIER;ZAMPIERON, 2005;BOCCHETTI et al, 2008), Mya (DOPP et al,1996), Perna (SIU et al, 2008), Crassostrea (BOLOGNESI et al, 2006) e Dressena (SIU et al, 2004;PAVLICA et al, 2000;BINELLI et al, 2010) como organismos indicadores para a avaliação dos compostos genotóxicos e de seus efeitos sobre ambientes marinhos e, também, de água doce. Esses estudos detectaram diferentes compostos capazes de gerar alterações cromossômicas.…”

Section: Organismos De Ambientes Aquáticos Utilizados Como Bioindicadunclassified

Avaliação E Monitoramento Da Qualidade Das Águas: Usando Análises Moleculares

Brito¹,

Luz²

2015

Revista GESTA

View full text Add to dashboard Cite

ResumoOs ambientes aquáticos têm sofrido com o aporte continuado de compostos químicos que afetam as relações ecológicas de várias formas. Para se conhecer o quão é afetado o ecossistema, são necessários programas de monitoramento mais completos, contínuos e com a utilização de biomarcadores moleculares. Este trabalho objetiva auxiliar na escolha de bioindicadores para análises ambientais de corpos d´água, e estimular o uso de procedimentos moleculares (biomarcadores). Os testes moleculares são importantes para verificar o nível clastogênico de compostos químicos sobre o material genético dos indivíduos. Aqui são apresentados dois testes muito utilizados: o teste de Micronúcleo (MN), já estabelecido e adaptado aos organismos aquáticos, detecta pequenos núcleos, ao lado do principal, resultado de quebras dos cromossomos; e o teste de cometa, mais recentemente consolidado, é capaz de detectar fragmentações do DNA imperceptíveis ao teste de MN. Ambos os testes são rápidos e práticos de serem executados e de baixo custo, com ótimos resultados em espécies nativas. Com a retomada da necessidade de uma visão mais unificada dos processos ambientais, torna-se necessária a utilização de ferramentas que permitam uma visão holística do efeito dos compostos químicos no ambiente e, para isso, os testes moleculares são armas poderosas de monitoramento quando utilizados juntamente com as análises tradicionais.Palavras chave: ambientes aquáticos, bioindicador, micronúcleos, testes moleculares, poluição. AbstractAquatic environments have suffered from the continued input of chemicals affecting ecological relationships in several ways. In order to know the extent in which ecosystems are compromised, more complete and continued monitoring programs are necessary, including the use of molecular biomarkers. This work aims to assist in the choice of biomarkers for environmental analysis of water bodies, and considers two types of molecular procedures (biomarkers) widely used in recent years to verify the clastogenic level of chemicals on the genetic material of individuals. The micronucleus test (MN), already established and adapted to aquatic organisms, detects small nuclei close to the main nucleusas a result from chromosomes breakage. The comet test, most recently established, is able to detect the DNA fragmentation imperceptible to the MN. Both tests are quick and practical to be run and inexpensive as well, with great results in native species.With the resumption of the need for a more unified view of environmental processes, it is necessary to use tools that enable a holistic view of the effect of chemicals on the environment and, therefore, molecular tests are powerful weapons monitoring when used together with traditional analysis.

show abstract

Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol

Cited by 107 publications

References 34 publications

Effect of titanium dioxide nanoparticles on the bioavailability, metabolism, and toxicity of pentachlorophenol in zebrafish larvae

Effect of titanium dioxide nanoparticles on the bioavailability, metabolism, and toxicity of pentachlorophenol in zebrafish larvae

Evaluation of DNA damage induced by decabromodiphenyl ether (BDE‐209) in hemocytes of Dreissena polymorpha using the comet and micronucleus assays

Avaliação E Monitoramento Da Qualidade Das Águas: Usando Análises Moleculares

Contact Info

Product

Resources

About