Detection of microorganismic flows by linear and nonlinear optical methods and automatic correction of erroneous images artefacts and moving boundaries in image generating methods by a neuronumerical hybrid implementing the Taylor’s hypothesis as a priori knowledge

Petermeier, Hannes; Kowalczyk, Wojciech; Delgado, Antonio; Denz, Cornélia; Holtmann, Frank

doi:10.1007/s00348-007-0269-3

Cited by 15 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This flow shape is also similar to other species of stalked ciliates. 7,[10][11][12]15 The flow fields are similar in that they both create filterfeeding currents by metachronal beating of the cilia of the paroral and oral polykinetid 1. Our measured suction inflow of 307 pl/s is close to the values generated by protozoans which are 1 nl/s ͑Ref.…”

Section: Discussionmentioning

confidence: 93%

“…[5][6][7][8][9][10][11][12] However, to our knowledge, a three-dimensional ͑3D͒ velocimetry method to measure the flows generated by microorganisms has not yet been reported. The dimensions of the measurements have been limited to two-dimensional ͑2D͒ two components.…”

Section: Introductionmentioning

confidence: 99%

“…The other type of velocimetry image-based method is particle image velocimetry ͑PIV͒, an optical method to measure velocities by calculating the correlation between two images. Hartmann et al, 9 Kowalczyk et al, 10 Petermeier et al, 11 and Kondratieva et al 12 applied PIV to microorganisms and studied flow fields created by Opercularia asymmetrica. They measured 2D two-component flows in the vicinity of the microorganism.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9][10][11][12] The first method is particle tracking velocimetry ͑PTV͒, a technique to measure the velocity of a flow-driven particle. Sleigh and Aiello applied PTV to microorganisms and followed the movement of water around the cilia of Vorticella 6 and Stentor 7 by tracing the motion of latex particles with a frame-by-frame analyses of cine films obtained with microscope and a high speed camera.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Three-dimensional two-component velocity measurement of the flow field induced by the Vorticella picta microorganism using a confocal microparticle image velocimetry technique

et al. 2009

View full text Add to dashboard Cite

Understanding the biological feeding strategy and characteristics of a microorganism as an actuator requires the detailed and quantitative measurement of flow velocity and flow rate induced by the microorganism. Although some velocimetry methods have been applied to examine the flow, the measured dimensions were limited to at most two-dimensional two-component measurements. Here we have developed a method to measure three-dimensional two-component flow velocity fields generated by the microorganism Vorticella picta using a piezoscanner and a confocal microscope. We obtained the two-component velocities of the flow field in a two-dimensional plane denoted as the XY plane, with an observation area of 455ϫ 341 m 2 and the resolution of 9.09 m per each velocity vector by a confocal microparticle image velocimetry technique. The measurement of the flow field at each height took 37.5 ms, and it was repeated in 16 planes with a 2.50 m separation in the Z direction. We reconstructed the three-dimensional twocomponent flow velocity field. From the reconstructed data, the flow velocity field ͓u ͑x,y,z͒ , v ͑x,y,z͒ ͔ in an arbitrary plane can be visualized. The flow rates through YZ and ZX planes were also calculated. During feeding, we examined a suction flow to the mouth of the Vorticella picta and measured it to be to 300 pl/s.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Three-dimensional two-component velocity measurement of the flow field induced by the Vorticella picta microorganism using a confocal microparticle image velocimetry technique

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Experimental observations of several different sessile filter feeders have shown that they generate feeding currents in the form of a toroidal vortex (Sleigh & Aiello 1972;Sleigh & Barlow 1976;Pettitt et al 2002;Hartmann et al 2007;Petermeier et al 2007;Nagai et al 2009). Closed feeding streamlines limit the feeder's access to new material (Blake & Otto 1996), so it is important to determine what circumstances lead to closed eddies.…”

Section: Introductionmentioning

confidence: 99%

Nearby boundaries create eddies near microscopic filter feeders

Pepper

Roper

Ryu

et al. 2009

J. R. Soc. Interface.

View full text Add to dashboard Cite

We show through calculations, simulations and experiments that the eddies often observed near sessile filter feeders are frequently due to the presence of nearby boundaries. We model the common filter feeder Vorticella, which is approximately 50 mm across and which feeds by removing bacteria from ocean or pond water that it draws towards itself. We use both an analytical stokeslet model and a Brinkman flow approximation that exploits the narrow-gap geometry to predict the size of the eddy caused by two parallel no-slip boundaries that represent the slides between which experimental observations are often made. We also use three-dimensional finite-element simulations to fully solve for the flow around a model Vorticella and analyse the influence of multiple nearby boundaries. Additionally, we track particles around live feeding Vorticella in order to determine the experimental flow field. Our models are in good agreement both with each other and with experiments. We also provide approximate equations to predict the experimental eddy sizes owing to boundaries both for the case of a filter feeder between two slides and for the case of a filter feeder attached to a perpendicular surface between two slides.

show abstract

Biocompatible Visualization of Flow Fields Generated by Microorganisms

Zima-Kulisiewicz

Botello-Payro

Delgado

2009

Notes on Numerical Fluid Mechanics and Multidisciplinary Design

Self Cite

View full text Add to dashboard Cite

Abstract. Microflow induced by the ciliates called Opercularia asymmetrica is an interesting phenomenon in biofluidmechanics. Ciliates play an important role in the structural formation of microbial granules derived from activated sludge. Additionally, flow induced by protozoa (ciliates) is treated as an efficient mean of nutrient transport with minimum energy requirement. For the first time powerful digital imaging techniques are used for studying microorganismic convection. Investigations of the flow generated by Opercularia asymmetrica are carried out with help of digital micro Particle Image Velocimetry. Digital micro Particle Tracking Velocimetry is implemented to analyse cilia motion. In biological fluid mechanics flow visualization techniques must guarantee biocompatibility. Thus, in the present work appropriate light illumination and suitable seeding particles are used. Moreover, in order to predict artefacts and correct them novel neuronumerical hybrid is employed.

show abstract

Detection of microorganismic flows by linear and nonlinear optical methods and automatic correction of erroneous images artefacts and moving boundaries in image generating methods by a neuronumerical hybrid implementing the Taylor’s hypothesis as a priori knowledge

Cited by 15 publications

References 23 publications

Three-dimensional two-component velocity measurement of the flow field induced by the Vorticella picta microorganism using a confocal microparticle image velocimetry technique

Three-dimensional two-component velocity measurement of the flow field induced by the Vorticella picta microorganism using a confocal microparticle image velocimetry technique

Nearby boundaries create eddies near microscopic filter feeders

Biocompatible Visualization of Flow Fields Generated by Microorganisms

Contact Info

Product

Resources

About