Detection of microorganisms in different growth states based on microcalorimetry

An alternative sterility assessment for parenteral drug products using isothermal microcalorimetry

“…Isothermal microcalorimetry (IMC) is a method capable to detect heat produced by biochemically active cells such as growing bacteria or fungi (Tan et al . ; Braissant et al . ).…”

“…Growth detection of the RMM are based on electro-optical, spectroscopic or biochemical reaction analysis which all have advantages and drawbacks (Thorpe et al 1990;Chollet et al 2008;London et al 2010;Parveen et al 2011;Jimenez et al 2012;Celsis 2017), and are often invasive by nature (Sandle 2016;rapidmicromethods 2017). Isothermal microcalorimetry (IMC) is a method capable to detect heat produced by biochemically active cells such as growing bacteria or fungi (Tan et al 2012;Braissant et al 2013). In theory, with the most sensitive instrument available, already 20 nW (10 4 cells)-200 nW (10 5 cells) are sufficient to allow noninvasive detection of microbial activity by IMC (Braissant et al 2010).…”

Drug Micro-Carriers Based on Polymers and Their Sterilization

“…Therefore, some of these systems are used in direct contact with body fluids, such as sublingual administration, via the gastrointestinal tract (oral), vagina and nasal cavity, and all non-parenteral drug delivery routes [28][29][30][31][32][33][34][35][36][37][38][39]. Thus, they must comply with the requirements of the pharmacopeia for sterility [40][41][42][43][44]. However, some proposed sterilization methods, such as chemical sterilization with ethylene oxide, are not suitable for biodegradable aliphatic polyesters [45][46][47][48].…”

Microcalorimetric study of the growth of Enterococcus faecalis, Pseudomonas aeruginosa and their mixtures in an enriched culture medium

“…In the medical field, this method is very interesting due to the fast-growing nature of microorganisms and the resultant amount of heat that is soon produced [4]. In the last few years, it has been used to study the rapid detection of bacterial growth under different conditions [6][7][8][9] or microbial contamination [10][11][12]. Also, it has been employed to determine inhibitory effects and/or the minimal inhibitory concentration for different antimicrobial substances [13][14][15][16] and antibiotics [17][18][19] in a few hours.…”