Detection of minimal disease in breast cancer

Bosma, AJ; Helgason, Helgi H.; Braaf, LM; Rodenhuis, S.; Veer, L. van 't

doi:10.1186/bcr1183

Cited by 1 publication

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative realtime PCR was performed using 0.25 ml cDNA per reaction of 50 PCR cycles (Bosma et al, 2002). For FGF10, we used the same primers as in the RT-PCR, with the SYBR s Green PCR Master Mix from Applied Biosystems.…”

Section: Real-time Pcr For Human Carcinomasmentioning

confidence: 99%

Fgf10 is an oncogene activated by MMTV insertional mutagenesis in mouse mammary tumors and overexpressed in a subset of human breast carcinomas

et al. 2004

View full text Add to dashboard Cite

Mouse mammary tumor virus (MMTV) infection causes a high incidence of murine mammary carcinomas by insertion of its proviral DNA in the genome of mammary epithelial cells. Retroviral insertion can activate flanking proto-oncogenes by a process called insertional mutagenesis. By sequencing the DNA adjacent to MMTV proviral insertions in mammary tumors from BALB/c mice infected with C3H-MMTV, we have found a common MMTV insertion site in the Fgf10 locus. RT-PCR studies showed that Fgf10 is expressed only in those tumors harboring a MMTV proviral insertion in this locus, suggesting that Fgf10 is a proto-oncogene. The oncogenicity of Fgf10 was evaluated in vivo by subcutaneous transplantation of retrovirally transduced HC11 mammary epithelial cells into BALB/c mice. Highly vascularized invasive subcutaneous tumors developed indicating that Fgf10 can act as an oncogene. A survey of primary human breast carcinomas revealed strongly elevated Fgf10 mRNA levels in approximately 10% of the tumors tested, suggesting that Fgf10 may also be involved in oncogenicity of a subset of human breast cancers.

show abstract

Section: Real-time Pcr For Human Carcinomasmentioning

confidence: 99%