Detection of minimal residual disease

Deptała, Andrzej; Mayer, S

doi:10.1016/s0091-679x(01)64023-8

Cited by 6 publications

(5 citation statements)

References 63 publications

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“…MRD is the persistence of malignant cells in the bone marrow or other tissues after remission has been achieved by conventional morphologic assessment (318,319). It is believed that these residual cells are the source of relapse in many patients who achieve ''complete'' morphologic remission from acute leukemia or other malignant hematologic diseases, although additional therapy to eradicate very small numbers of residual cells does not improve survival in all patients.…”

Section: Detection Of Mrdmentioning

confidence: 99%

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

Riley

Williams

Ross

et al. 2009

Clinical Laboratory Analysis

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Bone marrow examination has become increasingly important for the diagnosis and treatment of hematologic and other illnesses. Morphologic evaluation of the bone marrow aspirate and biopsy has recently been supplemented by increasingly sophisticated ancillary assays, including immunocytochemistry, cytogenetic analysis, flow cytometry, and molecular assays. With our rapidly expanding knowledge of the clinical and biologic diversity of leukemia and other hematologic neoplasms, and an increasing variety of therapeutic options, the bone marrow examination has became more critical for therapeutic monitoring and planning optimal therapy. Sensitive molecular techniques, in vitro drug sensitivity testing, and a number of other special assays are available to provide valuable data to assist these endeavors. Fortunately, improvements in bone marrow aspirate and needle technology has made the procurement of adequate specimens more reliable and efficient, while the use of conscious sedation has improved patient comfort. The procurement of bone marrow specimens was reviewed in the first part of this series. This paper specifically addresses the diagnostic interpretation of bone marrow specimens and the use of ancillary techniques.

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Section: Detection Of Mrdmentioning

confidence: 99%

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

Riley

Williams

Ross

et al. 2009

Clinical Laboratory Analysis

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“…Advances in flow cytometer designs and novel fluorochromes for the chemical conjugation of antibodies have facilitated robust, multi-parametric analysis at a single cell level. The technology has been widely used in basic research (8), clinical analysis of patient immune responses (9), hematological malignancies and minimal residue disease (10)(11)(12), and enumeration of lymphocyte populations in immunodeficient diseases and cancer (13,14).…”

Section: Introductionmentioning

confidence: 99%

Development of a standardized flow cytometric method to conduct longitudinal analyses of intracellular CD3ζ expression in patients with head and neck cancer

2016

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Abstract. Head and neck squamous cell carcinoma (HNSCC) is the sixth most common neoplasm in the world. The follow-up protocols currently available do not appear to diagnose treatment failures and recurrences early enough to provide the best treatment to improve the survival rates of patients. The identification of biomarkers may aid in diagnosing, monitoring the progression, or predicting treatment outcomes in HNSCC. The present study aimed to evaluate whether cluster of differentiation (CD) 3ζ chain expression may serve as a biomarker for the early detection of recurrent or persistent HNSCC. However, in a longitudinal study, a standardized method that allows consistent data comparisons in an inter-assay manner is critical. The present study reveals a method to monitor expression levels of CD3ζ over multiple time-points using flow cytometry. The present study validated the use of an internal control and normalization procedure for tracking alterations in CD3ζ expression in samples from patients with HNSCC, which were collected and assayed for a longitudinal study.

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“…By capturing the scattered light and fluorescence emission, physical and chemical properties of the cells can be determined and cells can be sorted and counted. This high throughput technique is used in a variety of biomedical (Harding et al 2000;Boeck 2001) and clinical diagnostic applications (Stein et al 1992;Fenili and Pirovano 1998) including bacterial analysis (Gunasekera et al 2000), analysis of cell cycle and apoptosis (Darzynkiewicz et al 2001), detection of minimal residual disease (MRD) in leukemia cases (Deptala and Mayer 2001), and enumeration of CD4+ T-cells in HIV diagnosis (Cheng et al 2007). Additionally, flow cytometers can be used purely in cell sorting applications (Melamed et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Inertial microfluidics for sheath-less high-throughput flow cytometry

Bhagat

Kuntaegowdanahalli

Kaval

et al. 2009

Biomed Microdevices

196

157

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Flow cytometer is a powerful single cell analysis tool that allows multi-parametric study of suspended cells. Most commercial flow cytometers available today are bulky, expensive instruments requiring high maintenance costs and specially trained personnel for operation. Hence, there is a need to develop a low cost, portable alternative that will aid in making this powerful research tool more accessible. In this paper we describe a sheath-less, on-chip flow cytometry system based on the principle of Dean coupled inertial microfluidics. The design takes advantage of the Dean drag and inertial lift forces acting on particles flowing through a spiral microchannel to focus them in 3-D at a single position across the microchannel cross-section. Unlike the previously reported micro-flow cytometers, the developed system relies entirely on the microchannel geometry for particle focusing, eliminating the need for complex microchannel designs and additional microfluidic plumbing associated with sheath-based techniques. In this work, a 10-loop spiral microchannel 100 microm wide and 50 microm high was used to focus 6 microm particles in 3-D. The focused particle stream was detected with a laser induced fluorescence (LIF) setup. The microfluidic system was shown to have a high throughput of 2,100 particles/sec. Finally, the viability of the developed technique for cell counting was demonstrated using SH-SY5Y neuroblastoma cells. The passive focusing principle and the planar nature of the described design will permit easy integration with existing lab-on-a-chip (LOC) systems.

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Detection of minimal residual disease

Cited by 6 publications

References 63 publications

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

Development of a standardized flow cytometric method to conduct longitudinal analyses of intracellular CD3ζ expression in patients with head and neck cancer

Inertial microfluidics for sheath-less high-throughput flow cytometry

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