Detection of mixed Leishmania infections in dogs from an endemic area in southeastern Brazil

Souza, N. A. de; Leite, Rodrigo Souza; Silva, Soraia de Oliveira; Penna, Michele Groenner; Vilela, Liza Figueiredo Felicori; Melo, Maria Norma; Andrade, Antero Silva Ribeiro de

doi:10.1016/j.actatropica.2019.02.016

Cited by 19 publications

(8 citation statements)

References 26 publications

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“…Positive cats subjected to xenodiagnosis assays can infect the Leishmania vector (Maroli et al, 2007) and Leishmania braziliensis can be detected in skin samples from experimentally chronically infected cats (Simões-Mattos et al, 2005). Recently, in the same region of the present study, L. braziliensis DNA was detected in conjunctival swab of dogs (Souza et al, 2019), highlighting the importance of detecting Leishmania species not only in dogs but also in cats, to better understand the role of these hosts in the epidemiology of tegumentary leishmaniasis.…”

Section: Discussionsupporting

confidence: 58%

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

Costa-Val

Coura

Barbieri

et al. 2020

Rev. Bras. Parasitol. Vet.

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Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serological and molecular results (k = 0.16) was obtained. From five positive PCR samples one was L. braziliensis and four were L. infantum. Phylogenetic analysis performed with the five isolates of Leishmania, showed that samples of L. infantum isolated from the cats were phylogenetically close to those isolated from domestic dogs in Brazil, while the L. braziliensis is very similar to the one described in humans in Venezuela. The study demonstrated that, despite high seropositivity for Leishmania in cats living in the study region, poor agreement between serological and molecular results indicate that positive serology is not indicative of Leishmania infection in cats. Parasite DNA can be detected in ocular conjunctiva and oral swabs from cats, indicating that such samples could be used for diagnosis. Results of phylogenetic analyzes show that L. infantum circulating in Brazil is capable of infecting different hosts, demonstrating the parasite's ability to overcome the interspecies barrier.

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Section: Discussionsupporting

confidence: 58%

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

Costa-Val

Coura

Barbieri

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…(Madeira et al, 1999). Although L. infantum is implicated in canine leishmaniasis, the detection of other species of Leishmania in naturally infected dogs has been reported (Sanches et al, 2016;Valdivia et al, 2017;Alves Souza et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

DH82 Canine and RAW264.7 Murine Macrophage Cell Lines Display Distinct Activation Profiles Upon Interaction With Leishmania infantum and Leishmania amazonensis

Nadaes

Costa

Santana

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Although L. amazonensis is broadly documented as cause of CL, there are a few studies reporting that it is able to disseminate to cause VL in dogs and humans . Regarding BALB/c mice, some studies described parasite burden in lymph nodes and spleen significantly lower than paws after 30 days of infection .…”

Section: Discussionmentioning

confidence: 99%

Preclinical Investigation of Methylene Blue‐mediated Antimicrobial Photodynamic Therapy on Leishmania Parasites Using Real‐Time Bioluminescence

Cabral

Sabino

Dimmer

et al. 2019

Photochem & Photobiology

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Cutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB)‐mediated APDT (MB‐APDT) on Leishmania amazonensis in vitro and in vivo by bioluminescence technique. In vitro, MB‐APDT was performed using a red LED (λ = 660 ± 11 nm, 100 mW cm−2) and MB (100 µm) at different light doses. In vivo, mice were infected and 4 weeks later, randomly divided into three groups: control, APDT 1 (single session) and APDT 2 (two sessions of MB‐APDT). MB was used at 100 µm and energy dose was established at 150 J cm−2. Parasite burden, lesion size and pain were evaluated weekly for 4 weeks. In vitro, lethal dose for 90% parasite inactivation was achieved at 48.8 J cm−2. In vivo, although APDT 1 and APDT 2 groups have showed similar parasite burden after 4 weeks, two sessions were clinically better, especially considering the inflammatory process associated to CL. Our findings reinforce MB‐APDT as a cost‐effective treatment to combat CL.

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Detection of mixed Leishmania infections in dogs from an endemic area in southeastern Brazil

Cited by 19 publications

References 26 publications

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

DH82 Canine and RAW264.7 Murine Macrophage Cell Lines Display Distinct Activation Profiles Upon Interaction With Leishmania infantum and Leishmania amazonensis

Preclinical Investigation of Methylene Blue‐mediated Antimicrobial Photodynamic Therapy on Leishmania Parasites Using Real‐Time Bioluminescence

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