Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations

Slameñová, D; Gábelová, Alena; Ruzeková, L; Chalupa, Ivan; Horváthová, Eva; Farkašová, Timea; Bozsakyová, Eva; Štětina, Rudolf

doi:10.1016/s0921-8777(97)00007-4

Cited by 103 publications

(28 citation statements)

References 16 publications

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“…The method of single cell gel electrophoresis (SCGE; comet assay) according to [9] was performed with minor modifications suggested in [10,11]. Briefly: 2.5 x 10 4 AT-MSCs or CDy-AT-MSCs in 85μl of 0.75% low melting point agarose in PBS were spread on a base layer of 100 μl 1% normal-melting point agarose in PBS, placed on microscope slides and covered with a cover slip.…”

Section: Methodsmentioning

confidence: 99%

Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation

Altanerová¹,

Horváthová²,

Matúšková³

et al. 2009

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Human adipose tissue-derived mesenchymal (stromal) stem cells (AT-MSCs) and genetically modified to express cytosine deaminase:uracil phosphoribosyltransferase (CDy-AT-MSCs) were treated with hydrogen peroxide in order to induce DNA damage and subsequently evaluate their genetic stability by single cell gel electrophoresis. Both cells types (parental and transgene modified) did not differ in the sensitivity to DNA breaks induction. Potential tumorigenicity of AT-MSCs and CDy-AT-MSCs was tested by subcutaneous inoculation of cell suspension into flank of immunocompromised mice. Dose of 15x10 6 cells was not found to be tumorigenic in given experimental setup. AT-MSCs, CDy-AT-MSCs and MSCs isolated from human lipoma were treated with chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) in attempts to transform them. Surviving cells after genotoxic stress were not transformed but underwent replicative senescence. Irreparable DNA damage caused triggered adipogenic terminal differentiation, rather than apoptosis induction in all kinds of cells tested.

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Section: Methodsmentioning

confidence: 99%

Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation

Altanerová¹,

Horváthová²,

Matúšková³

et al. 2009

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“…We evaluated DNA-damaging (genotoxic) and DNA-protective effects of 24-h treatment of HepG2 and Caco-2 cells with five different compounds of essential oils: eucalyptol (0-5000 µM), eugenol (0-600 µM), borneol (0-1500 µM), carvacrol (0-300 µM) and thymol (0-200 µM). The level of DNA lesions was measured by standard alkaline single cell gel electrophoresis (SCGE; comet assay) suggested by Singh et al [60] and modified by Slamenova et al [61] and Gabelova et al [62]. At concentrations ≤IC 50 the compounds studied did not induce DNA strand breaks either in human HepG2 or in Caco-2 cells, however, incubation of human cells in the presence of the monoterpens thymol and carvacrol led to a significant protection of cellular DNA against a strong oxidative agent -hydrogen peroxide [47].…”

Section: Mutagenicity Anti-mutagenicity and Dna-protective Effectsmentioning

confidence: 99%

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Slameñová¹,

Horváthová²

2013

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Flowers, berries, leaves, barks and roots of different plants have been used through the ages as a source of flavor in food and perfume preparations. The volatiles responsible for the flavor of botanicals can be extracted from the plant material as "essential oils" (EOs), called also volatile oils or ethereal oils. The term essential is intended to indicate that the oil is the fragrant essence of the plant from which it is extracted. EOs are constituted by hydrocarbons (monoterpenes and sesquiterpenes) and oxygenated compounds (alcohols, esters, ethers, aldehydes, ketones, lactones, phenols and phenol ethers). Of the numerous groups of naturally occurring compounds examined so far terpenes are known as fragrances and flavoring agents. The data reported in this review including the data obtained in our laboratory show that many of EOs exhibit a range of biological activities inclusive of antioxidative, anti-mutagenic and anti-carcinogenic activities. Most of them belong to phytochemicals with chemopreventive potential. On the other hand some herbal products can cause serious adverse effects. A complex research of toxic, genotoxic, anti-mutagenic and anti-carcinogenic effects of EOs is therefore very important.

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“…45 A relationship between the significantly increased frequency of chromosome aberrations (CA) and cancer risk was demonstrated in spite of CA unspecificity. 46 Studies proved the Comet assay to be more sensitive than CA 47 and a very strong association was shown between the two types of damage, 47,48 suggesting that the Comet assay responses may clearly predict cytogenetic damage.…”

Section: Introductionmentioning

confidence: 98%

Amifostine (WR-2721) selective protection against melphalan genotoxicity

et al. 2000

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Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations

Cited by 103 publications

References 16 publications

Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation

Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

Amifostine (WR-2721) selective protection against melphalan genotoxicity

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