Detection of mosquito saliva‐specific IgE antibodies by capture ELISA

Brummer‐Korvenkontio, Henrikki; Palosuo, Kati; Palosuo, Timo; Brummer-Korvenkontio, M; Leinikki, Pauli; Reunala, Timo

doi:10.1111/j.1398-9995.1997.tb01002.x

Cited by 33 publications

(33 citation statements)

References 17 publications

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“…Elevated serum mosquito saliva-specific IgE has also been detected by immunosorbent assays which include enzyme-linked immunoassays (ELISA) (fig. 1) [2, 5, 20], RAST [18], and the UniCap test [21], Western blot [22, 23], and histamine release from basophils [19]in mosquito-allergic or mosquito bite test-positive subjects. There is no doubt that IgE is involved in the pathogenesis of mosquito allergy.…”

Section: Immune Mechanismsmentioning

confidence: 99%

Mosquito Allergy: Immune Mechanisms and Recombinant Salivary Allergens

Peng

Simons²

2004

Int Arch Allergy Immunol

124

105

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Reactions to mosquito bites are immunological in nature, with the involvement of IgE-, IgG- and T lymphocyte-mediated hypersensitivities. Acquired desensitization to mosquito saliva may occur during childhood and adolescence or during long-term exposure to mosquito bites. Due to the lack of salivary preparations, allergic reactions to mosquito bites are underdiagnosed and undertreated. Recombinant mosquito saliva allergens with biological activity are being developed. These recombinant allergens will significantly improve diagnosis of mosquito allergy and eventually will also improve specific immunotherapy for patients with systemic reactions to mosquito bites.

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Section: Immune Mechanismsmentioning

confidence: 99%

Mosquito Allergy: Immune Mechanisms and Recombinant Salivary Allergens

Peng

Simons²

2004

Int Arch Allergy Immunol

124

105

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“…These studies demonstrated that the quantitative evaluation of antibody responses to saliva by enzyme-linked immunosorbent assay (ELISA) could be a useful biomarker for human exposure to Aedes bites. 23,24 IgG4 to Aedes aegypti saliva was associated with intense exposure to Aedes bites. 25 Recently, IgM and IgG responses to Ae.…”

mentioning

confidence: 99%

Human Antibody Response to Aedes aegypti Saliva in an Urban Population in Bolivia: A New Biomarker of Exposure to Dengue Vector Bites

Doucouré¹,

Mouchet²,

Cournil³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Aedes mosquitoes are important vectors of re-emerging diseases in developing countries, and increasing exposure to Aedes in the developed world is currently a source of concern. Given the limitations of current entomologic methods, there is a need for a new effective way for evaluating Aedes exposure. Our objective was to evaluate specific antibody responses to Aedes aegypti saliva as a biomarker for vector exposure in a dengue-endemic urban area. IgG responses to saliva were strong in young children and steadily waned with age. Specific IgG levels were significantly higher in persons living in sites with higher Ae. aegypti density, as measured by using entomologic parameters. Logistic regression showed a significant correlation between IgG to saliva and exposure level, independently of either age or sex. These results suggest that antibody responses to saliva could be used to monitor human exposure to Aedes bites.

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“…Possibly the naturally acquired immunity by repeated exposure is crucial [17], e.g. IgE and IgG antibodies to allergens of several mosquito species have been found [15, 18]. Typically the levels are highest in children and slowly decrease over time.…”

Section: Systemic Reactions To Arthropodsmentioning

confidence: 99%

Systemic Immediate Allergic Reactions to Arthropod Stings and Bites

Bircher

2005

Dermatology

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Most of the encounters with biting and stinging insects result in more or less pronounced localized reactions. Typically, urticarial wheals and papular reactions are observed. Less often local bullous or hemorrhagic or disseminated papular reactions, particularly in children and immunologically naive adults, may be seen. With the exception of bee and wasp venom allergies, immediate-type allergic reactions to arthropod stings and bites are rare. Systemic IgE-mediated hypersensitivity has also been reported from additional hymenoptera species, e.g. hornets, bumble bees and ants. Rare are systemic reactions to mosquitoes, flies or kissing bugs and exceptional from ticks, bed bugs, moths, caterpillars and spiders. A major problem is the often lacking standardization of extracts for skin testing and for the determination of specific IgE. Some of the allergens have been characterized and few of them synthesized using recombinant techniques. Most investigations have been made with whole-body extracts or extracts from salivary glands, while desensitization has rarely been attempted. Currently, primary prevention by avoidance of stings and bites, and adequate instruction of sensitized individuals in the use of emergency drugs are mandatory.

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Detection of mosquito saliva‐specific IgE antibodies by capture ELISA

Cited by 33 publications

References 17 publications

Mosquito Allergy: Immune Mechanisms and Recombinant Salivary Allergens

Mosquito Allergy: Immune Mechanisms and Recombinant Salivary Allergens

Human Antibody Response to Aedes aegypti Saliva in an Urban Population in Bolivia: A New Biomarker of Exposure to Dengue Vector Bites

Systemic Immediate Allergic Reactions to Arthropod Stings and Bites

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