Detection of mRNA for Nuclear Receptor Co-Activators 1 and 3 in Archival Breast Cancer Tissue and Surrounding Stroma: A Tissue Expression Study~!2009-01-14~!2010-02-10~!2010-07-20~!

Abstract

Before the age of 75 years, approximately 10% of women will be diagnosed with breast cancer, one of the most common malignancies and a leading cause of death among women. The objective of this study was to determine if expression of the nuclear receptor coactivators 1 and 3 (NCoA1 and NCoA3) varied in breast cancer grades. RNA was extracted from 25 breast tumours and transcribed into cDNA which underwent semi-quantitative polymerase chain reaction, normalised using 18S. Analysis indicated that an expression change for NCoA1 in cancer grades and estrogen receptor alpha negative tissue (P= 0.028 and 0.001 respectively). NCoA1 expression increased in grade 3 and estrogen receptor alpha negative tumours, compared to controls. NCoA3 showed a similar, but not significant, trend in grade and a non-significant decrease in estrogen receptor alpha negative tissues. Expression of NCoA1 in late stage and estrogen receptor alpha negative breast tumours may have implications to breast cancer treatment, particularly in the area of manipulation of hormone signalling systems in advanced tumours.

Investigation of the 1758G>C and 2880A>G variants within the NCOA3 gene in a breast cancer affected Australian population

“…NCOA3 was selected as a suitable candidate gene due to its high impact in breast cancer in several studies including those by Burwinkel et al (2005), Smith et al (2010), Reiter et al (2001) and Anzick et al (1997).…”

“…Research in our laboratory recently demonstrated that NCOA3 expression increases in grade 3 and oestrogen receptor alpha negative tumours, when compared to controls (Smith et al, 2010). While NCOA3 is reported to be over-expressed in many breast cancer patients, Burwinkel et al identified two SNPs 1758G N C (rs2230782) and 2880A N G (rs2076546) that appeared to have a protective effect (Burwinkel et al, 2005).…”