Detection Of Multiple Human Papillomavirus Types In Condylomata Acuminata From Immunosuppressed Patients

Abstract

Using the hybrid capture method, condylomata acuminata from healthy patients (controls) and patients with altered cell-mediated immunity were analyzed for multiple human papillomavirus (HPV) DNA: 82.9% and 38.0% of lesions from 41 controls and 21 patients, respectively, were HPV DNA-positive only with probes for low-risk HPV types (P = .00035). Using probes for both low- and high-risk HPV types, 16.3% and 52.3% of lesions from 43 controls and 21 patients, respectively, were positive for both probes (P = .0038). Evidence of multiple HPV types was also found by Southern blot and in situ hybridization studies. The mean HPV copy number detected by either probe did not differ significantly among patient groups. Using sensitive techniques, such as hybrid capture, multiple HPV types, including those associated with genital malignancy, can be detected in condylomata acuminata. Serial biopsies demonstrate the dynamic nature of genital HPV infection and that changes in the predominant HPV types may be reflected in tissue pathology.

Detection of specific human papillomavirus types in paraffin‐embedded sections of cervical carcinomas

“…Compared to condylomata acuminata lesions, in which greater than 50% contain multiple HPV types [Brown et al, 1994], cervical cancers in the current study were infected with multiple types in less than 16% of cases.…”

“…A semiquantitative 5-point (1þ through 5þ) scoring system was designed based on the low-and high-signal b-globin bands [Brown et al, 1999]. The low positive b-globin band was assigned a value of 2, and the high positive band was assigned a value of 4.…”

A Longitudinal Study of Genital Human Papillomavirus Infection in a Cohort of Closely Followed Adolescent Women

“…To determine specimen adequacy, the GH20 and PC04 human β-globin targets were coamplified. Reactions were amplified in a Perkin Elmer TC9600 Thermal Cycler (Perkin Elmer), as described elsewhere [7]. DNA isolated from a condyloma acuminata lesion was used as a positive control.…”

“…Bovine serum albumin-conjugated probes for each HPV type are deposited in a single line. Hybridization and detection of PCR products bound to immobilized probes was performed as described elsewhere [7].…”

Incidence and Duration of Cervical Human Papillomavirus 6, 11, 16, and 18 Infections in Young Women: An Evaluation from Multiple Analytic Perspectives

“…HPV infections can lead to cancers and precancers of the cervix (2), anus, penis, vagina and vulva (3,4), anogenital warts (5,6), and recurrent respiratory papillomatosis (7,8), with growing evidence for a role in the pathogenesis of cancers of the head and neck (9). A diagnosis of HPV can lead to distress (10), shame, and anger and negatively affect sexual activity and enjoyment (11).…”