Detection of Multiple Potato Viruses in the Field Suggests Synergistic Interactions among Potato Viruses in Pakistan

Hameed, Amir; Iqbal, Zafar; Asad, Shaheen; Mansoor, Shahid

doi:10.5423/ppj.oa.05.2014.0039

Cited by 48 publications

(32 citation statements)

References 33 publications

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“…As a rule, several viruses in one plant can coexist if they belong to different groups. Moreover, if the titer of a more virulent pathogen remains at the same level, the titer of a less virulent pathogen grows during the growing season of the plant (Nyalugwe et al, 2012;Hameed et al, 2014). The results presented here are not consistent with the previous findings.…”

Section: Resultscontrasting

confidence: 99%

Development of a new method for eradication of viruses by induction of posttranscriptional gene silencing in transgenic potato plants

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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A new method of improving potato plants from a viral infection has been proposed. DNA fragments containing the full coding sequence of PVS 25K protein in sense and antisense orientations were cloned from 5' and 3' ends of intron I of Ricinus communis catalase gene cat1 Ricinus communis into binary agrobacterial vector pCAMBIA2300 under control of 35S CaMV promoter and terminator of nopalin synthase gene. Using the recombinant DNA as a transgene, transformation of six varieties of test-tube potato plants, which were infected with PVM, PVS and/or PVY individually or in complex, was carried out. Further field trials of 2017-2018 allowed us to select four promising transgenic lines that were free from viral infection.

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Section: Resultscontrasting

confidence: 99%

Development of a new method for eradication of viruses by induction of posttranscriptional gene silencing in transgenic potato plants

2019

Plant Genetic Resources for Breeding and Producing Functional Nutraceutical Food

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“…Loss of the results by 50% was occurred due to a mixed infection between PVX and PVY (Brunt, 2001). The decrease in farmers' harvests can be attributed to the presence of viral infection in plants, where disease progression will be more severe and higher viral accumulation in mixed infections was compared with single infections of these viruses (Hameed et al, 2014).…”

Section: Serology Test Resultsmentioning

confidence: 99%

“…This Good potato cultivation requires quality and certified seeds where the seed price is high enough. Generally, the farmers use local seeds from generation to generation to more than 4th generation, so they have a high chance of investing in viruses that contain 6-7 times of the virus and can decrease the potato production result (Mulyana, 2005;Pradjadinata, 2005).…”

Section: Introductionmentioning

confidence: 99%

DETECTION AND ELIMINATION VIRUS ON POTATO (Solanum tuberosum L.)

Margareth

Astarini

Temaja

2018

Int. J. Biosci. Biotech.

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This study aims to determine the symptoms of virus attacks on potato crops, identification of viruses that attack potato plants in Pancasari village, Bali, efectivity of thermotherapy technique to eliminate in vivo potato. The research was taken using purposive sampling technique and complete random program. Virus symptoms were observed on two potato fields. Serological test employed DAS-ELISA with PVX, PVS, PVY and PLRV antiserum. In vivo elimination virus were done by thermoteraphy in 37°C±1°C (4 days), 34°C±1°C (3 days) on 3 weeks and 33°C±1°C (4 days), 30°C±1°C (3 days) on 2 weeks. Results of the study at Pancasari Village Bali shown varied symptoms on potato leaves, such as mottle, mosaic, malformation, leafroll, witchesbroom. Through serological test, had identified viruses were 11.11% PVX, 55.56% PVS, 80% PVY and 11.11% PLRV which were infected farmer’s potato. In vivo thermotherapy was succeed decreasing PVS and PVY level during 2 weeks on 33°C±1°C (4 days) and 30°C±1°C (3 days).

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“…Infeksi ganda PVY dan PVX terjadi secara sistemik pada semua bagian tanaman kentang. Infeksi ganda virus RNA pada tanaman kentang dapat menyebabkan peningkatan epidemi penyakit yang lebih tinggi dibandingkan dengan infeksi tunggal (Hameed et al 2014). Infeksi dimulai dari bagian daun, kemudian menyebar ke semua bagian tanaman, termasuk umbi.…”

Section:  Pengamatan Dengan Tingkat Intensitas Penyakit Yang Berbedaunclassified

Yield Loss Estimation of Dwarf Disease on Potato in Central Java

Fitriyati

Mutaqin

Damayanti

2020

JIPI

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ABSTRAKKentang merupakan salah satu komoditas yang penting secara ekonomi di Indonesia. Akan tetapi, produksinya berfluktuasi karena berbagai faktor yang memengaruhi hasil panen. Baru-baru ini ditemukan penyakit kerdil pada pertanaman kentang di Kabupaten Banjarnegara dan Wonosobo, Jawa Tengah. Sampai saat ini, belum ada informasi tentang kehilangan hasil yang disebabkan oleh penyakit tersebut. Tujuan penelitian ini adalah untuk menaksasi kehilangan hasil yang disebabkan oleh penyakit kerdil pada tanaman kentang di Jawa Tengah. Taksasi kehilangan hasil ditentukan secara aktual pada beberapa lahan dengan intensitas penyakit yang berbeda. Pengamatan dilakukan selama lima kali dengan interval dua minggu pada lima lahan pertanaman dan pada individual tanaman. Kuantitas dan kualitas hasil panen serta harga jual dihitung pada akhir pengamatan. Data dianalisis dengan analisis regresi dan korelasi untuk menentukan hubungan dan korelasi antara keparahan penyakit dan ABKPP (Area Bawah Kurva Perkembangan Penyakit) dengan kehilangan hasil. Penyakit kerdil menyebabkan penurunan tinggi tanaman, bobot umbi, dan bobot total umbi, tetapi tidak berpengaruh pada jumlah umbi hasil panen. Intensitas penyakit yang berbeda pada beberapa lahan berpengaruh pada persentase kehilangan hasil. Kehilangan hasil panen kentang akibat penyakit kerdil di lapangan per individu tanaman mencapai 64,42%. Kehilangan hasil tanaman kentang per petak pengamatan di lapangan pada tingkat intensitas penyakit yang berbeda berkisar antara 10,3526,43%. Analisis regresi antara ABKPP berdasarkan keparahan penyakit dan tingkat kehilangan hasil menunjukkan R square yang tinggi, yaitu 88,6% dan menunjukkan korelasi sebesar 94,14%. Hal ini mengindikasikan bahwa terdapat korelasi yang tinggi antara keparahan penyakit dan kehilangan hasil.Kata kunci: harga jual, hasil panen, keparahan penyakit, kualitas umbi ABSTRACT Potato is one of economically important crops in Indonesia. However, its production fluctuates since there are some factors affecting the yield. Recently, we found dwarf disease at potato central production area in Wonosobo and Banjarnegara Regencies in Central Java. So far, there is no information related to the yield loss caused by the dwarf disease on potato. Therefore, the research aimed to estimate the yield loss caused by the dwarf disease on potato in Central Java. The yield loss estimation was determined on some potato cultivations with different disease severities. The disease intensity was observed for five times with interval two weeks on six plots. The quantity and quality of potato yields, and selling price was calculated at the end of the observation and the correlation between the severity of disease and AUDPC (Area under disease progress curve) with yield loss was determined by regression and correlation analysis. The dwarf disease reduced plant height, individual and total tuber weight without affecting the number of tuber yield. The different of disease intensities in some area affected to the percentage of yield loss. The yield loss ca...

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Detection of Multiple Potato Viruses in the Field Suggests Synergistic Interactions among Potato Viruses in Pakistan

Cited by 48 publications

References 33 publications

Development of a new method for eradication of viruses by induction of posttranscriptional gene silencing in transgenic potato plants

Development of a new method for eradication of viruses by induction of posttranscriptional gene silencing in transgenic potato plants

DETECTION AND ELIMINATION VIRUS ON POTATO (Solanum tuberosum L.)

Yield Loss Estimation of Dwarf Disease on Potato in Central Java

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